scholarly journals Interactions between polycyclic aromatic hydrocarbons in binary mixtures: Effects on gene expression and DNA adduct formation in precision-cut rat liver slices

Mutagenesis ◽  
2008 ◽  
Vol 23 (6) ◽  
pp. 491-499 ◽  
Author(s):  
Y. C. M. Staal ◽  
D. S. Pushparajah ◽  
M. H. M. van Herwijnen ◽  
R. W. H. Gottschalk ◽  
L. M. Maas ◽  
...  
Keyword(s):  
Gene Expression ◽  
Polycyclic Aromatic Hydrocarbons ◽  
Binary Mixtures ◽  
Rat Liver ◽  
Aromatic Hydrocarbons ◽  
Adduct Formation ◽  
Dna Adduct ◽  
Liver Slices ◽  
Polycyclic Aromatic

Exposure-route-dependent DNA adduct formation by polycyclic aromatic hydrocarbons

2000 ◽  
Vol 21 (1) ◽  
pp. 87-92 ◽  
Author(s):  
Roger W.L. Godschalk ◽  
Edwin J.C. Moonen ◽  
Pauline A.E.L. Schilderman ◽  
Wendy M.R. Broekmans ◽  
Jos C.S. Kleinjans ◽  
...  
Keyword(s):  
Polycyclic Aromatic Hydrocarbons ◽  
Aromatic Hydrocarbons ◽  
Adduct Formation ◽  
Dna Adduct ◽  
Exposure Route ◽  
Polycyclic Aromatic

DNA adduct formation of benzo[a]pyrene in white blood cells of workers exposed to polycyclic aromatic hydrocarbons

2005 ◽  
Vol 208 (3) ◽  
pp. 173-178 ◽  
Author(s):  
Thomas Mensing ◽  
Boleslaw Marczynski ◽  
Beate Engelhardt ◽  
Michael Wilhelm ◽  
Ralf Preuss ◽  
...  
Keyword(s):  
Polycyclic Aromatic Hydrocarbons ◽  
Aromatic Hydrocarbons ◽  
Blood Cells ◽  
White Blood Cells ◽  
Adduct Formation ◽  
Dna Adduct ◽  
Polycyclic Aromatic

scholarly journals Exposure-route-dependent DNA adduct formation by polycyclic aromatic hydrocarbons

2000 ◽  
Vol 21 (1) ◽  
pp. 87-92 ◽  
Author(s):  
Roger W.L. Godschalk ◽  
Edwin J.C. Moonen ◽  
Pauline A.E.L. Schilderman ◽  
Wendy M.R. Broekmans ◽  
Jos C.S. Kleinjans ◽  
...  
Keyword(s):  
Polycyclic Aromatic Hydrocarbons ◽  
Aromatic Hydrocarbons ◽  
Adduct Formation ◽  
Dna Adduct ◽  
Exposure Route ◽  
Polycyclic Aromatic

scholarly journals The Effects of Everyday-Life Exposure to Polycyclic Aromatic Hydrocarbons on Biological Age Indicators

2020 ◽  
Author(s):  
Sofia Pavanello ◽  
Manuela Campisi ◽  
Giuseppe Mastrangelo ◽  
Mirjam Hoxha ◽  
Valentina Bollati
Keyword(s):  
Polycyclic Aromatic Hydrocarbons ◽  
Everyday Life ◽  
Aromatic Hydrocarbons ◽  
Adduct Formation ◽  
Dna Adduct ◽  
Leukocyte Telomere Length ◽  
Mitochondrial Dna Copy Number ◽  
Age Related ◽  
Polycyclic Aromatic ◽  
Pah Exposure

Abstract BackgroundFurther knowledge on modifiable aging risk factors is required to mitigate the increasing burden of age-related diseases in a rapidly growing global demographic of elderly individuals. We explored the effect of everyday exposure to carcinogenic polycyclic aromatic hydrocarbons (PAHs), which are fundamental constituents of air pollution, on cellular biological aging. This was determined via the analysis of leukocyte telomere length (LTL), mitochondrial DNA copy number (LmtDNAcn), and by the formation of anti- benzo[a]pyrene diolepoxide (B[a]PDE–DNA) adducts.MethodsThe study population consisted of 585 individuals living in North-East Italy. PAH exposure (diet, indoor activities, outdoor activities, traffic, and residential exposure) and smoking behavior were assessed by questionnaire and through measures of target dose [B[a]PDE–DNA]. LTL, LmtDNAcn and genetic polymorphisms [glutathione S-transferase M1 and T1 (GSTM1; GSTT1)] were measured by polymerase chain reaction methods. Structural equation modelling analysis evaluated these complex relationships.ResultsIn two models investigating LTL and LmtDNAcn, we found an ordered relationship between these measurements and PAH exposure (diet, indoor, outdoor, traffic, residential). Acting through anti-B[a]PDE–DNA adduct formation, we detected reduced LTL (p=0.028) and LmtDNAcn (p=0.018) levels, particularly in males (LTL p=0.006; LmtDNAcn p=0.0001). Active smoking only increased LTL (p=0.0001). Besides this, the most significant determinants of PAH exposure causing an increase in anti-B[a]PDE–DNA were indoor and diet (p=0.0001 for each). while the least significant was outdoor. Lastly, the presence of detoxifying GSTM1 decreased adduct levels.Conclusion New findings stemming from our study suggest that LTL and LmtDNAcn erosion depends on certain preventable everyday life exposure to PAHs. In particular, the clear association with indoor activities, diet, and gender opens new perspectives for tailored preventive measures in age-related diseases, including lung cancer.Capsule:Everyday life exposure to polycyclic aromatic hydrocarbons shortens leukocyte telomere length and mitochondrial DNA copy number through anti-B[a]PDE-DNA adduct formation.

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