Cytochrome P450 induction by nitrated polycyclic aromatic hydrocarbons, azaarenes, and binary mixtures in fish hepatoma cell line PLHC-1

2001 ◽  
Vol 20 (1) ◽  
pp. 149-159 ◽  
Author(s):  
Detlev K. J. Jung ◽  
Thomas Klaus ◽  
Karl Fent
Keyword(s):  
Cytochrome P450 ◽  
Polycyclic Aromatic Hydrocarbons ◽  
Cell Line ◽  
Binary Mixtures ◽  
Aromatic Hydrocarbons ◽  
Hepatoma Cell ◽  
Hepatoma Cell Line ◽  
Nitrated Polycyclic Aromatic Hydrocarbons ◽  
Cytochrome P450 Induction ◽  
Polycyclic Aromatic

Use of the Rainbow Trout Hepatoma Cell Line, RTH-149, in a Cytotoxicity Assay

1989 ◽  
Vol 17 (2) ◽  
pp. 67-71
Author(s):  
Harvey Babich ◽  
Nieves Martin-Alguacil ◽  
Ellen Borenfreund
Keyword(s):  
Polycyclic Aromatic Hydrocarbons ◽  
Rainbow Trout ◽  
Cell Line ◽  
Aromatic Hydrocarbons ◽  
Hepatoma Cell ◽  
Neutral Red ◽  
Cytotoxicity Assay ◽  
Hepatoma Cell Line ◽  
Polycyclic Aromatic ◽  
Direct Acting

The rainbow trout hepatoma cell line, RTH-149, was evaluated for use as a bioindicator cell type in the neutral red cytotoxicity assay. The cells were exposed for six days to various polycyclic aromatic hydrocarbons, including chemicals that are direct-acting toxicants and chemicals that require enzymatic biotransformation to cytotoxic metabolites. Whereas benzo[a]pyrene was only slightly cytotoxic, its metabolites — (±)trans-7,8-diol-benzo[a]pyrene and 3-hydroxy-benzo[a]pyrene — were highly cytotoxic. 7,12-Dimethylbenz[a]anthracene was cytotoxic, but cytotoxicity did not occur with benzo[a]anthracene, benzo[b]fluoranthene and benzo[k]fluoranthene. This cell line appears to lack sufficient xenobiotic metabolising capacity to biotransform many of these polycyclic aromatic hydrocarbons to activated cytotoxic metabolites.

Biodegradation of Polycyclic Aromatic Hydrocarbons Using FungiNew Prospects toward Cytochrome P450 Engineering

2018 ◽  
pp. 417-445
Author(s):  
Ulises Conejo-Saucedo ◽  
Darío Rafael Olicón-Hernández ◽  
Haley Paula Stein ◽  
Jesús González-López ◽  
Elisabet Aranda
Keyword(s):  
Cytochrome P450 ◽  
Polycyclic Aromatic Hydrocarbons ◽  
Aromatic Hydrocarbons ◽  
Polycyclic Aromatic

scholarly journals Increased Cytotoxicity of Carbon Tetrachloride in a Human Hepatoma Cell Line Overexpressing Cytochrome P450 2E1

2002 ◽  
Vol 30 (4) ◽  
pp. 400-405 ◽  
Author(s):  
S Takahashi ◽  
T Takahashi ◽  
S Mizobuchi ◽  
M Matsumi ◽  
K Morita ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Cytochrome P450 ◽  
Carbon Tetrachloride ◽  
Cell Line ◽  
Hepatoma Cell ◽  
Mrna Level ◽  
Hepatoma Cell Line ◽  
Cytochrome P450 2E1 ◽  
Hsp70 Mrna ◽  
Potential Marker

Cytotoxic free radicals generated during the metabolism of carbon tetrachloride by cytochrome P450 2E1 (CYP2E1) are thought to cause hepatotoxicity. Here, the cytotoxic effects of carbon tetrachloride in a liver cell line expressing CYP2E1 (HLE/2E1) are compared with those in the mother cell line (HLE). The effects of carbon tetrachloride on the gene expression of HSP70, a potential marker of oxidative stress, were also examined. The viability of HLE/2E1 cells after exposure to carbon tetrachloride was significantly decreased compared with that of HLE cells. Northern blot analysis revealed that the HSP70 mRNA level was significantly increased after carbon tetrachloride treatment in both cell lines, while the magnitude of its increase was much greater in HLE/2E1 cells than in HLE cells. These results suggest that the oxidative stress induced by CYP2E1 plays an important role in the increase in cytotoxicity of carbon tetrachloride in CYP2E1-overexpressing cells.

Sign in / Sign up

Export Citation Format

Share Document