scholarly journals Influence of mesenchymal stem cells on stomach tissue engineering using small intestinal submucosa

2013 ◽  
Vol 9 (3) ◽  
pp. 296-304 ◽  
Author(s):  
Hiroki Nakatsu ◽  
Tomio Ueno ◽  
Atsunori Oga ◽  
Mitsuhiro Nakao ◽  
Taku Nishimura ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Small Intestinal Submucosa ◽  
Stomach Tissue ◽  
Small Intestinal ◽  
Intestinal Submucosa

Human urine-derived stem cells seeded in a modified 3D porous small intestinal submucosa scaffold for urethral tissue engineering

Biomaterials ◽  
2011 ◽  
Vol 32 (5) ◽  
pp. 1317-1326 ◽  
Author(s):  
Shaofeng Wu ◽  
Yan Liu ◽  
Shantaram Bharadwaj ◽  
Anthony Atala ◽  
Yuanyuan Zhang
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Human Urine ◽  
Small Intestinal Submucosa ◽  
Small Intestinal ◽  
Intestinal Submucosa

scholarly journals Effects of hypoxia on Achilles tendon repair using adipose tissue-derived mesenchymal stem cells seeded small intestinal submucosa

2021 ◽  
Vol 16 (1) ◽  
Author(s):  
Xing Guo ◽  
Hui Lv ◽  
ZhongWei Fan ◽  
Ke Duan ◽  
Jie Liang ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Achilles Tendon ◽  
Peak Load ◽  
Small Intestinal Submucosa ◽  
Sham Operation ◽  
Small Intestinal ◽  
Intestinal Submucosa ◽  
Achilles Tendon Defects ◽  
Group D

Abstract Background The study was performed to evaluate the feasibility of utilizing small intestinal submucosa (SIS) scaffolds seeded with adipose-derived mesenchymal stem cells (ADMSCs) for engineered tendon repairing rat Achilles tendon defects and to compare the effects of preconditioning treatments (hypoxic vs. normoxic) on the tendon healing. Methods Fifty SD rats were randomized into five groups. Group A received sham operation (blank control). In other groups, the Achilles tendon was resected and filled with the original tendon (Group B, autograft), cell-free SIS (Group C), or SIS seeded with ADMSCs preconditioned under normoxic conditions (Group D) or hypoxic conditions (Group E). Samples were collected 4 weeks after operation and analyzed by histology, immunohistochemistry, and tensile testing. Results Histologically, compared with Groups C and D, Group E showed a significant improvement in extracellular matrix production and a higher compactness of collagen fibers. Group E also exhibited a significantly higher peak tensile load than Groups D and C. Additionally, Group D had a significantly higher peak load than Group C. Immunohistochemically, Group E exhibited a significantly higher percentage of MKX + cells than Group D. The proportion of ADMSCs simultaneously positive for both MKX and CM-Dil observed from Group E was also greater than that in Group D. Conclusions In this animal model, the engineered tendon grafts created by seeding ADMSCs on SIS were superior to cell-free SIS. The hypoxic precondition further improved the expression of tendon-related genes in the seeded cells and increased the rupture load after grafting in the Achilles tendon defects.

Sign in / Sign up

Export Citation Format

Share Document