Decellularization of bovine small intestinal submucosa and its use for the healing of a critical-sized full-thickness skin defect, alone and in combination with stem cells, in a small rodent model

2015 ◽  
Vol 11 (6) ◽  
pp. 1754-1765 ◽  
Author(s):  
Mahmut Parmaksiz ◽  
A. Eser Elcin ◽  
Y. Murat Elcin
Keyword(s):  
Stem Cells ◽  
Small Intestinal Submucosa ◽  
Rodent Model ◽  
Small Rodent ◽  
Skin Defect ◽  
Full Thickness ◽  
Full Thickness Skin ◽  
Thickness Skin ◽  
Small Intestinal ◽  
Intestinal Submucosa

scholarly journals Concentrated Hypoxia-Preconditioned Adipose Mesenchymal Stem Cell-Conditioned Medium Improves Wounds Healing in Full-Thickness Skin Defect Model

2014 ◽  
Vol 2014 ◽  
pp. 1-6 ◽  
Author(s):  
Biao Sun ◽  
Shilei Guo ◽  
Fei Xu ◽  
Bin Wang ◽  
Xiujuan Liu ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Conditioned Medium ◽  
Skin Defect ◽  
Full Thickness ◽  
Defect Model ◽  
Full Thickness Skin ◽  
Thickness Skin ◽  
Freeze Dried ◽  
Adipose Mesenchymal Stem Cells

In recent years, the bioactive factors were utilized in exercise and athletic skin injuries. In this research, the concentrated conditioned medium of hypoxia-preconditioned adipose mesenchymal stem cells, which is rich in bioactive factor, is applied in full-thickness skin defect model to evaluate the therapeutic efficacy. Adipose mesenchymal stem cells were harvested from the abdominal subcutaneous adipose tissues. The surface markers and the potential of differentiation were analyzed. The conditioned medium of hypoxia-preconditioned stem cells was collected and freeze-dried and then applied on the rat full-thickness skin defect model, and the healing time of each group was recorded. Haematoxylin and eosin staining of skin was assessed by microscope. The characteristics of adipose mesenchymal stem cells were similar to those of other mesenchymal stem cells. The concentration of protein in freeze-dried conditioned medium in 1 mL water was about 15 times higher than in the normal condition medium. In vivo, the concentrated hypoxia-preconditioned conditioned medium can reduce the wound size and accelerate the skin wound healing. The concentrated hypoxia-preconditioned adipose mesenchymal stem cell-conditioned medium has great effect on rat model of wound healing, and it would be an ideal agent for wound care in clinical application.

scholarly journals Experimental evaluation of the effectiveness of adipose mesenchymal stem cells in full-thickness skin wounds

2021 ◽  
Vol 9 (2) ◽  
pp. 175-181
Author(s):  
Ilya V. Artsimovich ◽  
Evgenii V. Zinoviev ◽  
Aleksander V. Gostimskii ◽  
Marat S. Asadulaev ◽  
Sergey V. Vissarionov ◽  
...  
Keyword(s):  
Stem Cells ◽  
Wound Healing ◽  
Mesenchymal Stem Cells ◽  
Control Group ◽  
Skin Defect ◽  
Full Thickness ◽  
Skin Defects ◽  
Full Thickness Skin ◽  
Healing Index ◽  
Thickness Skin

BACKGROUND: Currently, numerous techniques or medical devices that allow complete or partial restoration of the lost skin within a short time remain as subjects of development. Many studies have shown the effectiveness of using stem cells in the treatment of full-thickness skin defects, but their use remains very limited. At present, there is no consensus among researchers about the advisability of the use of stem cells in the treatment of burns as well as about the method of their introduction. AIM: This study aimed to examine the possibility of accelerating the reparative histogenesis of tissues in the zone of deep skin burns using cultures of adipogenic mesenchymal cells, as well as to evaluate the effectiveness of various methods of introducing cultures of these cells into the defect zone. MATERIALS AND METHODS: An experimental study was carried out on small laboratory animals (rats). After simulating a grade III burn, mesenchymal stem cells were transplanted and superficially applied to the wound surface or injected under the fascia. In the control group, no wound treatment was performed. To assess the effectiveness of the wound-healing preparations, the appearance of wounds was assessed daily, the nature of the discharge and presence and type of granulations were noted, and the timing of scab rejection and wound healing was recorded. The wound area was assessed using a planimetric method. A histological examination of wound biopsies was carried out on days 7, 14, 21, and 28 of observation. RESULTS: The application of adipogenic mesenchymal stem cells demonstrated the greatest efficiency on the developed burn model. Subfascial administration was less effective, but this method achieved a significant acceleration of wound healing in comparison with the control group. An increase in the healing index by 56.6% demonstrates the highest intensity of reparative regeneration in animals applied with adipogenic mesenchymal stem cells. CONCLUSIONS: The preliminary results show that the application of adipogenic mesenchymal stem cells on the skin defect is more effective than subfascial administration based on the healing index. The use of adipogenic mesenchymal stem cells may substantially increase the effectiveness of the treatment of full-thickness skin defects.

Human urine-derived stem cells seeded in a modified 3D porous small intestinal submucosa scaffold for urethral tissue engineering

Biomaterials ◽  
2011 ◽  
Vol 32 (5) ◽  
pp. 1317-1326 ◽  
Author(s):  
Shaofeng Wu ◽  
Yan Liu ◽  
Shantaram Bharadwaj ◽  
Anthony Atala ◽  
Yuanyuan Zhang
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Human Urine ◽  
Small Intestinal Submucosa ◽  
Small Intestinal ◽  
Intestinal Submucosa

scholarly journals The role of placenta-derived mesenchymal stem cells in healing of induced full-thickness skin wound in a mouse model

IUBMB Life ◽  
2015 ◽  
Vol 67 (9) ◽  
pp. 701-709 ◽  
Author(s):  
Somia H. Abd-Allah ◽  
Amal S. El-Shal ◽  
Sally M. Shalaby ◽  
Eman Abd-Elbary ◽  
Nehad F. Mazen ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Mouse Model ◽  
Skin Wound ◽  
Full Thickness ◽  
Full Thickness Skin ◽  
Thickness Skin
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