Vascular hypervolemia and hematoma formation in chick embryos treated with trypan blue

Teratology ◽  
1981 ◽  
Vol 23 (3) ◽  
pp. 403-409 ◽  
Author(s):  
T. E. Weaver ◽  
S. Kaplan ◽  
G. M. Rajala
1967 ◽  
Vol 165 (3) ◽  
pp. 325-335 ◽  
Author(s):  
Stanley Kaplan ◽  
Casimer T. Grabowski
Keyword(s):  

Teratology ◽  
1968 ◽  
Vol 1 (4) ◽  
pp. 369-373 ◽  
Author(s):  
Stanley Kaplan ◽  
E. Marshall Johnson

Development ◽  
1960 ◽  
Vol 8 (1) ◽  
pp. 1-5
Author(s):  
Leela Mulherkar

Various malformations in rats and mice have been observed to be caused by administration of the vital dye, Trypan blue (Gillman, Gilbert, Gillman, & Spence, 1948; Fox & Goss, 1956, 1957; Hamburgh, 1952, 1954; Waddington & Carter, 1952, 1953). Recently Waddington & Perry (1956) reported a teratogenic effect of Trypan blue on Amphibian embryos. The present paper deals with the effects of this dye on cultured chick embryos. Hen's eggs were incubated at 38°C. to the desired stage of development. Cultures were set up according to the ring technique described by New (1955). Precautions to maintain sterile conditions were observed throughout. An aqueous 1 per cent, solution of Gurr's vital Trypan blue was diluted with Pannett Compton saline to 0·05 per cent., which had been found to be a suitable concentration. A few drops of the dye solution were placed on the upper surface of the treated blastoderms.


1974 ◽  
Vol 38 (2) ◽  
pp. 383-389 ◽  
Author(s):  
Gary L. Kolesari ◽  
Stanley Kaplan
Keyword(s):  

Teratology ◽  
1972 ◽  
Vol 5 (3) ◽  
pp. 315-318 ◽  
Author(s):  
David J. Hoffman ◽  
Gordon M. Ramm
Keyword(s):  

1972 ◽  
Vol 182 (2) ◽  
pp. 227-231 ◽  
Author(s):  
David J. Hoffman ◽  
Gordon M. Ramm

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