scholarly journals Prolonged Membrane Depolarization Enhances Midbrain Dopamine Neuron Differentiation via Epigenetic Histone Modifications

Stem Cells ◽  
2011 ◽  
Vol 29 (11) ◽  
pp. 1861-1873 ◽  
Author(s):  
Xi-Biao He ◽  
Sang-Hoon Yi ◽  
Yong-Hee Rhee ◽  
Hyemin Kim ◽  
Yong-Mahn Han ◽  
...  
Keyword(s):  
Histone Modifications ◽  
Membrane Depolarization ◽  
Dopamine Neuron ◽  
Neuron Differentiation ◽  
Midbrain Dopamine

scholarly journals Histamine Modulates Midbrain Dopamine Neuron Differentiation Through the Regulation of Epigenetic Marks

2019 ◽  
Vol 13 ◽  
Author(s):  
Fernanda Vargas-Romero ◽  
Rodrigo González-Barrios ◽  
Lissania Guerra-Calderas ◽  
Itzel Escobedo-Avila ◽  
Daniel Cortés-Pérez ◽  
...  
Keyword(s):  
Dopamine Neuron ◽  
Neuron Differentiation ◽  
Epigenetic Marks ◽  
Midbrain Dopamine

scholarly journals Gene regulatory logic of dopamine neuron differentiation

Nature ◽  
2009 ◽  
Vol 458 (7240) ◽  
pp. 885-889 ◽  
Author(s):  
Nuria Flames ◽  
Oliver Hobert
Keyword(s):  
Dopamine Neuron ◽  
Neuron Differentiation ◽  
Gene Regulatory

scholarly journals Purity and Enrichment of Laser-Microdissected Midbrain Dopamine Neurons

2013 ◽  
Vol 2013 ◽  
pp. 1-8 ◽  
Author(s):  
Amanda L. Brown ◽  
Trevor A. Day ◽  
Christopher V. Dayas ◽  
Doug W. Smith
Keyword(s):  
Gene Expression ◽  
Expression Profiles ◽  
Gene Expression Profiles ◽  
Dopamine Neuron ◽  
Dopamine Neurons ◽  
Acid Decarboxylase ◽  
Cell Groups ◽  
Midbrain Dopamine ◽  
High Degree ◽  
Midbrain Dopamine Neurons

The ability to microdissect individual cells from the nervous system has enormous potential, as it can allow for the study of gene expression in phenotypically identified cells. However, if the resultant gene expression profiles are to be accurately ascribed, it is necessary to determine the extent of contamination by nontarget cells in the microdissected sample. Here, we show that midbrain dopamine neurons can be laser-microdissected to a high degree of enrichment and purity. The average enrichment for tyrosine hydroxylase (TH) gene expression in the microdissected sample relative to midbrain sections was approximately 200-fold. For the dopamine transporter (DAT) and the vesicular monoamine transporter type 2 (Vmat2), average enrichments were approximately 100- and 60-fold, respectively. Glutamic acid decarboxylase (Gad65) expression, a marker for GABAergic neurons, was several hundredfold lower than dopamine neuron-specific genes. Glial cell and glutamatergic neuron gene expression were not detected in microdissected samples. Additionally, SN and VTA dopamine neurons had significantly different expression levels of dopamine neuron-specific genes, which likely reflects functional differences between the two cell groups. This study demonstrates that it is possible to laser-microdissect dopamine neurons to a high degree of cell purity. Therefore gene expression profiles can be precisely attributed to the targeted microdissected cells.

Molecular mechanisms underlying midbrain dopamine neuron development and function

2003 ◽  
Vol 480 (1-3) ◽  
pp. 75-88 ◽  
Author(s):  
Marten P. Smidt ◽  
Simone M. Smits ◽  
J.Peter H. Burbach
Keyword(s):  
Molecular Mechanisms ◽  
Dopamine Neuron ◽  
Neuron Development ◽  
Midbrain Dopamine ◽  
And Function

scholarly journals SFRP1 and SFRP2 Dose-Dependently Regulate Midbrain Dopamine Neuron Development In Vivo and in Embryonic Stem Cells

Stem Cells ◽  
2012 ◽  
Vol 30 (5) ◽  
pp. 865-875 ◽  
Author(s):  
Julianna Kele ◽  
Emma R. Andersson ◽  
J. Carlos Villaescusa ◽  
Lukas Cajanek ◽  
Clare L. Parish ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Embryonic Stem ◽  
Dopamine Neuron ◽  
Neuron Development ◽  
Midbrain Dopamine

scholarly journals Instantiation of incentive value and movement invigoration by distinct midbrain dopamine circuits

2017 ◽  
Author(s):  
Benjamin T. Saunders ◽  
Jocelyn M. Richard ◽  
Elyssa B. Margolis ◽  
Patricia H. Janak
Keyword(s):  
Dopamine Neuron ◽  
Dopamine Neurons ◽  
Neuron Activity ◽  
Temporal Association ◽  
Conditioned Stimuli ◽  
Incentive Value ◽  
Pavlovian Learning ◽  
Midbrain Dopamine ◽  
Dopamine Circuits

Environmental cues, through Pavlovian learning, become conditioned stimuli that guide animals towards the acquisition of “rewards” (i.e., food) that are necessary for survival. Here, we test the fundamental role of midbrain dopamine neurons in conferring predictive or motivational properties to cues, independent of external rewards. We demonstrate that phasic optogenetic excitation of dopamine neurons throughout the midbrain, when presented in temporal association with discrete sensory cues, is sufficient to instantiate those cues as conditioned stimuli that subsequently both evoke dopamine neuron activity on their own, and elicit cue-locked conditioned behaviors. Critically, we identify highly parcellated behavioral functions for dopamine neuron subpopulations projecting to discrete regions of striatum, revealing dissociable mesostriatal systems for the generation of incentive value and movement invigoration. These results show that dopamine neurons orchestrate Pavlovian conditioning via functionally heterogeneous, circuit-specific motivational signals to shape cue-controlled behavior.

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