scholarly journals Periarticular Mesenchymal Progenitors Initiate and Contribute to Secondary Ossification Center Formation During Mouse Long Bone Development

Stem Cells ◽  
2019 ◽  
Vol 37 (5) ◽  
pp. 677-689 ◽  
Author(s):  
Wei Tong ◽  
Robert J. Tower ◽  
Chider Chen ◽  
Luqiang Wang ◽  
Leilei Zhong ◽  
...  
Keyword(s):  
Bone Development ◽  
Long Bone ◽  
Ossification Center ◽  
Mesenchymal Progenitors ◽  
Secondary Ossification Center

Isolation, Culture and Identification of Bovine Skeletal Stem Cells

2021 ◽  
Vol 11 (12) ◽  
pp. 2337-2345
Author(s):  
Junhui Lai ◽  
Qin Yang ◽  
Ruining Liang ◽  
Weijun Guan ◽  
Xiuxia Li
Keyword(s):  
Stem Cells ◽  
Cell Surface ◽  
Bone Formation ◽  
Growth Plate ◽  
Bone Development ◽  
Long Bone ◽  
Biological Characterization ◽  
Self Renewal ◽  
And Cartilage

The growth plate is essential in long bone formation and contains a wealth of skeletal stem cells (SSCs). Though the origin and the mechanism for SSCs generation remain uncertain, recent studies demonstrate the transition from cartilage to bone that in the lineage for bone development. SSCs possesses the ability to differentiate into bone and cartilage in vitro. In this research, we aimed to isolate and culture the skeletal stem cells from bovine cattle and then studied its biological characterization. The results showed that these bovine SSCs are positive for PDPN+CD73+CD164+CD90+CD44+ cell surface bio-markers, they are capable of self-renewal and differentiation. Our dates proved that SSCs exists in bovine’s long bone.

Long-bone development and life-history traits of the Devonian tristichopterid Hyneria lindae

2018 ◽  
Vol 109 (1-2) ◽  
pp. 75-86 ◽  
Author(s):  
Viktoriia KAMSKA ◽  
Edward B. DAESCHLER ◽  
Jason P. DOWNS ◽  
Per E. AHLBERG ◽  
Paul TAFFOREAU ◽  
...  
Keyword(s):  
Life History ◽  
Limb Development ◽  
Life History Traits ◽  
Bone Development ◽  
Bone Histology ◽  
Long Bone ◽  
General Character ◽  
Appendicular Skeleton ◽  
Predatory Behaviour ◽  
Large Size

ABSTRACTHyneria lindae is one of the largest Devonian sarcopterygians. It was found in the Catskill Formation (late Famennian) of Pennsylvania, USA. The current study focuses on the palaeohistology of the humerus of this tristichopterid and supports a low ossification rate and a late ossification onset in the appendicular skeleton. In addition to anatomical features, the large size of the cell lacunae in the cortical bone of the humerus mid-shaft may suggest a large genome size and associated neotenic condition for this species, which could, in turn, be a partial explanation for the large size of H. lindae. The low metabolism of H. lindae revealed here by bone histology supports the hypothesis of an ambush predatory behaviour. Finally, the lines-of-arrested-growth pattern and late ossification of specimen ANSP 21483 suggest that H. lindae probably had a long juvenile stage before reaching sexual maturity. Although very few studies address the life-history traits of stem tetrapods, they all propose a slow limb development for the studied taxa despite different ecological conditions and presumably distinct behaviours. The bone histology of H. lindae would favour the hypothesis that a slow long-bone development could be a general character for stem tetrapods.

scholarly journals Rac signaling in osteoblastic cells is required for normal bone development but is dispensable for hematopoietic development

Blood ◽  
2012 ◽  
Vol 119 (3) ◽  
pp. 736-744 ◽  
Author(s):  
Steven W. Lane ◽  
Serena De Vita ◽  
Kylie A. Alexander ◽  
Ruchan Karaman ◽  
Michael D. Milsom ◽  
...  
Keyword(s):  
Bone Growth ◽  
Bone Development ◽  
Long Bone ◽  
Perivascular Niche ◽  
Hematopoietic Stem ◽  
Hsc Niche ◽  
Rac1 Gtpase

Abstract Hematopoietic stem cells (HSCs) interact with osteoblastic, stromal, and vascular components of the BM hematopoietic microenvironment (HM) that are required for the maintenance of long-term self-renewal in vivo. Osteoblasts have been reported to be a critical cell type making up the HSC niche in vivo. Rac1 GTPase has been implicated in adhesion, spreading, and differentiation of osteoblast cell lines and is critical for HSC engraftment and retention. Recent data suggest a differential role of GTPases in endosteal/osteoblastic versus perivascular niche function. However, whether Rac signaling pathways are also necessary in the cell-extrinsic control of HSC function within the HM has not been examined. In the present study, genetic and inducible models of Rac deletion were used to demonstrate that Rac depletion causes impaired proliferation and induction of apoptosis in the OP9 cell line and in primary BM stromal cells. Deletion of Rac proteins caused reduced trabecular and cortical long bone growth in vivo. Surprisingly, HSC function and maintenance of hematopoiesis in vivo was preserved despite these substantial cell-extrinsic changes. These data have implications for therapeutic strategies to target Rac signaling in HSC mobilization and in the treatment of leukemia and provide clarification to our evolving concepts of HSC-HM interactions.

scholarly journals Wnt signaling in chondroprogenitors during long bone development and growth

Bone ◽  
2020 ◽  
Vol 137 ◽  
pp. 115368
Author(s):  
Takeshi Oichi ◽  
Satoru Otsuru ◽  
Yu Usami ◽  
Motomi Enomoto-Iwamoto ◽  
Masahiro Iwamoto
Keyword(s):  
Wnt Signaling ◽  
Bone Development ◽  
Long Bone

The role of Has2 on long bone development

2008 ◽  
Vol 27 ◽  
pp. 22-23
Author(s):  
Peter J. Roughley ◽  
Judy Grover ◽  
Eunice R. Lee ◽  
Yu Yamaguchi
Keyword(s):  
Bone Development ◽  
Long Bone

scholarly journals Effects of Incubator Temperature and Oxygen Concentration During the Plateau Stage of Oxygen Consumption on Turkey Embryo Long Bone Development

2008 ◽  
Vol 87 (8) ◽  
pp. 1484-1492 ◽  
Author(s):  
E.O. Oviedo-Rondón ◽  
J. Small ◽  
M.J. Wineland ◽  
V.L. Christensen ◽  
J.L. Grimes ◽  
...  
Keyword(s):  
Oxygen Consumption ◽  
Oxygen Concentration ◽  
Bone Development ◽  
Long Bone

scholarly journals Loss of VHL in mesenchymal progenitors of the limb bud alters multiple steps of endochondral bone development

2014 ◽  
Vol 393 (1) ◽  
pp. 124-136 ◽  
Author(s):  
Laura Mangiavini ◽  
Christophe Merceron ◽  
Elisa Araldi ◽  
Richa Khatri ◽  
Rita Gerard-O’Riley ◽  
...  
Keyword(s):  
Bone Development ◽  
Limb Bud ◽  
Endochondral Bone ◽  
Mesenchymal Progenitors

scholarly journals Thyroid hormone receptor-β1 signaling is critically involved in regulating secondary ossification via promoting transcription of the Ihh gene in the epiphysis

2016 ◽  
Vol 310 (10) ◽  
pp. E846-E854 ◽  
Author(s):  
Weirong Xing ◽  
Patrick Aghajanian ◽  
Helen Goodluck ◽  
Chandrasekhar Kesavan ◽  
Shaohong Cheng ◽  
...  
Keyword(s):  
Thyroid Hormone ◽  
Proximal Tibia ◽  
Thyroid Hormone Receptor ◽  
Bone Development ◽  
Day Treatment ◽  
Mrna Level ◽  
Ossification Center ◽  
Mobility Shift ◽  
Distal Promoter

Thyroid hormone (TH) action is mediated through two nuclear TH receptors, THRα and THRβ. Although the role of THRα is well established in bone, less is known about the relevance of THRβ-mediated signaling in bone development. On ther basis of our recent finding that TH signaling is essential for initiation and formation of secondary ossification center, we evaluated the role of THRs in mediating TH effects on epiphysial bone formation. Two-day treatment of TH-deficient Tshr −/− mice with TH increased THRβ1 mRNA level 3.4-fold at day 7 but had no effect on THRα1 mRNA level at the proximal tibia epiphysis. Treatment of serum-free cultures of tibias from 3-day-old mice with T3 increased THRβ1 expression 2.1- and 13-fold, respectively, at 24 and 72 h. Ten-day treatment of Tshr −/− newborns ( days 5–14) with THRβ1 agonist GC1 at 0.2 or 2.0 μg/day increased BV/TV at day 21 by 225 and 263%, respectively, compared with vehicle treatment. Two-day treatment with GC1 (0.2 μg/day) increased expression levels of Indian hedgehog ( Ihh) 100-fold, osterix 15-fold, and osteocalcin 59-fold compared with vehicle at day 7 in the proximal tibia epiphysis. Gel mobility shift assay demonstrated that a putative TH response element in the distal promoter of mouse Ihh gene interacted with THRβ1. GC1 treatment (1 nM) increased Ihh distal promoter activity 20-fold after 48 h in chondroctyes. Our data suggest a novel role for THRβ1 in secondary ossification at the epiphysis that involves transcriptional upregulation of Ihh gene.

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