Massively Parallel Single-Molecule and Single-Cell Emulsion Reverse Transcription Polymerase Chain Reaction Using Agarose Droplet Microfluidics

Huifa Zhang; Gareth Jenkins; Yuan Zou; Zhi Zhu; Chaoyong James Yang

doi:10.1021/ac2033084

Massively Parallel Single-Molecule and Single-Cell Emulsion Reverse Transcription Polymerase Chain Reaction Using Agarose Droplet Microfluidics

Analytical Chemistry ◽

10.1021/ac2033084 ◽

2012 ◽

Vol 84 (8) ◽

pp. 3599-3606 ◽

Cited By ~ 93

Author(s):

Huifa Zhang ◽

Gareth Jenkins ◽

Yuan Zou ◽

Zhi Zhu ◽

Chaoyong James Yang

Keyword(s):

Polymerase Chain Reaction ◽

Single Cell ◽

Single Molecule ◽

Reverse Transcription ◽

Droplet Microfluidics ◽

Massively Parallel ◽

Chain Reaction ◽

Polymerase Chain

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Comparison of reverse transcription–quantitative polymerase chain reaction methods and platforms for single cell gene expression analysis

Analytical Biochemistry ◽

10.1016/j.ab.2012.05.010 ◽

2012 ◽

Vol 427 (2) ◽

pp. 178-186 ◽

Cited By ~ 14

Author(s):

Bridget C. Fox ◽

Alison S. Devonshire ◽

Marc-Olivier Baradez ◽

Damian Marshall ◽

Carole A. Foy

Keyword(s):

Gene Expression ◽

Polymerase Chain Reaction ◽

Single Cell ◽

Reverse Transcription ◽

Gene Expression Analysis ◽

Quantitative Polymerase Chain Reaction ◽

Chain Reaction ◽

Cell Gene Expression ◽

Polymerase Chain ◽

Cell Gene

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Single cell reverse transcription-polymerase chain reaction (RT-PCR) and the GABA-A receptor

Neurochemistry International ◽

10.1016/0197-0186(95)00076-3 ◽

1996 ◽

Vol 28 (2) ◽

pp. 137-139 ◽

Cited By ~ 4

Author(s):

Sterling N. Sudweeks ◽

Roy E. Twyman

Keyword(s):

Polymerase Chain Reaction ◽

Single Cell ◽

Reverse Transcription ◽

Rt Pcr ◽

Chain Reaction ◽

Gaba A ◽

Polymerase Chain

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Global Single-Cell Reverse Transcription-Polymerase Chain Reaction (GSC RT-PCR)

Encyclopedic Dictionary of Genetics, Genomics and Proteomics ◽

10.1002/0471684228.egp05136 ◽

2004 ◽

Keyword(s):

Polymerase Chain Reaction ◽

Single Cell ◽

Reverse Transcription ◽

Rt Pcr ◽

Chain Reaction ◽

Polymerase Chain

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Single Cell Reverse Transcription-Polymerase Chain Reaction Analysis of Rat Supraoptic Magnocellular Neurons: Neuropeptide Phenotypes and High Voltage-Gated Calcium Channel Subtypes

Endocrinology ◽

10.1210/endo.140.11.7136 ◽

1999 ◽

Vol 140 (11) ◽

pp. 5391-5401 ◽

Cited By ~ 60

Author(s):

Eric Glasgow ◽

Kiyoshi Kusano ◽

Hemin Chin ◽

Êva Mezey ◽

W. Scott Young ◽

...

Keyword(s):

Polymerase Chain Reaction ◽

Calcium Channel ◽

Single Cell ◽

High Voltage ◽

Reverse Transcription ◽

Polymerase Chain Reaction Analysis ◽

Chain Reaction ◽

Magnocellular Neurons ◽

Voltage Gated ◽

Polymerase Chain

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An Immunofluorescence-Assisted Microfluidic Single Cell Quantitative Reverse Transcription Polymerase Chain Reaction Analysis of Tumour Cells Separated from Blood

Journal of Circulating Biomarkers ◽

10.5772/61822 ◽

2015 ◽

Vol 4 ◽

pp. 11 ◽

Cited By ~ 3

Author(s):

Kazunori Hoshino ◽

HaeWon Chung ◽

Chun-Hsien Wu ◽

Kaarthik Rajendran ◽

Yu-Yen Huang ◽

...

Keyword(s):

Polymerase Chain Reaction ◽

Single Cell ◽

Reverse Transcription ◽

Polymerase Chain Reaction Analysis ◽

Tumour Cells ◽

Chain Reaction ◽

Polymerase Chain

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Generation of monoclonal antibodies using simplified single-cell reverse transcription-polymerase chain reaction and cell-free protein synthesis

Journal of Bioscience and Bioengineering ◽

10.1263/jbb.101.284 ◽

2006 ◽

Vol 101 (3) ◽

pp. 284-286 ◽

Cited By ~ 4

Author(s):

Muhamad Ali ◽

Kiyotaka Hitomi ◽

Hideo Nakano

Keyword(s):

Polymerase Chain Reaction ◽

Protein Synthesis ◽

Monoclonal Antibodies ◽

Single Cell ◽

Reverse Transcription ◽

Chain Reaction ◽

Free Protein ◽

Polymerase Chain ◽

Cell Free Protein Synthesis

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Gene expression in individual cells: analysis using global single cell reverse transcription polymerase chain reaction (GSC RT-PCR)

Mutation Research/Mutation Research Genomics ◽

10.1016/s1383-5726(98)00009-0 ◽

1999 ◽

Vol 406 (2-4) ◽

pp. 45-54 ◽

Cited By ~ 12

Author(s):

Leslie H Brail ◽

Anne Jang ◽

Filio Billia ◽

Norman N Iscove ◽

Henry J Klamut ◽

...

Keyword(s):

Gene Expression ◽

Polymerase Chain Reaction ◽

Single Cell ◽

Reverse Transcription ◽

Rt Pcr ◽

Chain Reaction ◽

Polymerase Chain

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Patch-Clamp and Single-Cell Reverse Transcription–Polymerase Chain Reaction/Microarray Analysis

Springer Protocols Handbooks - Patch Clamp Techniques ◽

10.1007/978-4-431-53993-3_24 ◽

2012 ◽

pp. 371-377

Author(s):

Akihiro Yamanaka

Keyword(s):

Polymerase Chain Reaction ◽

Patch Clamp ◽

Single Cell ◽

Microarray Analysis ◽

Reverse Transcription ◽

Chain Reaction ◽

Polymerase Chain

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Single-molecule reverse transcription polymerase chain reaction using water-in-oil emulsion

Journal of Bioscience and Bioengineering ◽

10.1263/jbb.99.293 ◽

2005 ◽

Vol 99 (3) ◽

pp. 293-295 ◽

Cited By ~ 35

Author(s):

Michihiko Nakano ◽

Naohito Nakai ◽

Hirofumi Kurita ◽

Jun Komatsu ◽

Kazunori Takashima ◽

...

Keyword(s):

Polymerase Chain Reaction ◽

Single Molecule ◽

Reverse Transcription ◽

Chain Reaction ◽

Oil Emulsion ◽

Polymerase Chain ◽

Water In Oil Emulsion

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Photoregulated oligonucleotide detachment as a way to improve the efficiency of polymerase chain reaction with reverse transcription for analysis at the single cell level

Immunologiya ◽

10.33029/0206-4952-2021-42-6-662-669 ◽

2021 ◽

Vol 42 (6) ◽

pp. 662-669

Author(s):

I.B. Kozlov ◽

◽

M.Yu. Lebedin ◽

G.O. Gudima ◽

I.V. Sergeev ◽

...

Keyword(s):

Polymerase Chain Reaction ◽

Single Cell ◽

Reverse Transcription ◽

Single Cell Level ◽

Cell Level ◽

Chain Reaction ◽

Polymerase Chain

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