scholarly journals Mastering Dendrimer Self-Assembly for Efficient siRNA Delivery: From Conceptual Design to In Vivo Efficient Gene Silencing

Small ◽  
2016 ◽  
Vol 12 (27) ◽  
pp. 3667-3676 ◽  
Author(s):  
Chao Chen ◽  
Paola Posocco ◽  
Xiaoxuan Liu ◽  
Qiang Cheng ◽  
Erik Laurini ◽  
...  
Keyword(s):  
Gene Silencing ◽  
Conceptual Design ◽  
Self Assembly ◽  
Sirna Delivery ◽  
Efficient Gene

scholarly journals siRNA Delivery: Mastering Dendrimer Self-Assembly for Efficient siRNA Delivery: From Conceptual Design to In Vivo Efficient Gene Silencing (Small 27/2016)

Small ◽  
2016 ◽  
Vol 12 (27) ◽  
pp. 3604-3604 ◽  
Author(s):  
Chao Chen ◽  
Paola Posocco ◽  
Xiaoxuan Liu ◽  
Qiang Cheng ◽  
Erik Laurini ◽  
...  
Keyword(s):  
Gene Silencing ◽  
Conceptual Design ◽  
Self Assembly ◽  
Sirna Delivery ◽  
Efficient Gene

scholarly journals Gold-DNA nanosunflowers for efficient gene silencing with controllable transformation

2019 ◽  
Vol 5 (10) ◽  
pp. eaaw6264 ◽  
Author(s):  
Shuaidong Huo ◽  
Ningqiang Gong ◽  
Ying Jiang ◽  
Fei Chen ◽  
Hongbo Guo ◽  
...  
Keyword(s):  
Gene Silencing ◽  
Self Assembly ◽  
Near Infrared ◽  
Irradiation Time ◽  
Nucleic Acid Delivery ◽  
Efficient Gene ◽  
Efficient Delivery ◽  
Triplex Forming Oligonucleotide

The development of an efficient delivery system for enhanced and controlled gene interference–based therapeutics is still facing great challenges. Fortunately, the flourishing field of nanotechnology provides more effective strategies for nucleic acid delivery. Here, the triplex-forming oligonucleotide sequence and its complementary strand were used to mediate self-assembly of ultrasmall gold nanoparticles. The obtained sunflower-like nanostructures exhibited strong near-infrared (NIR) absorption and photothermal conversion ability. Upon NIR irradiation, the large-sized nanostructure could disassemble and generate ultrasmall nanoparticles modified with c-myc oncogene silencing sequence, which could directly target the cell nucleus. Moreover, the controlled gene silencing effect could be realized by synergistically controlling the preincubation time with the self-assembled nanostructure (in vitro and in vivo) and NIR irradiation time point. This study provides a new approach for constructing more efficient and tailorable nanocarriers for gene interference applications.

scholarly journals In vivo gene silencing following non-invasive siRNA delivery into the skin using a novel topical formulation

2014 ◽  
Vol 196 ◽  
pp. 355-362 ◽  
Author(s):  
Vikas Hegde ◽  
Robyn P. Hickerson ◽  
Sitheswaran Nainamalai ◽  
Paul A. Campbell ◽  
Frances J.D. Smith ◽  
...  
Keyword(s):  
Gene Silencing ◽  
Sirna Delivery ◽  
Topical Formulation ◽  
Non Invasive

Biocompatible, chimeric peptide-condensed supramolecular nanoparticles for tumor cell-specific siRNA delivery and gene silencing

2014 ◽  
Vol 50 (58) ◽  
pp. 7806-7809 ◽  
Author(s):  
Hangxiang Wang ◽  
Wei Chen ◽  
Haiyang Xie ◽  
Xuyong Wei ◽  
Shengyong Yin ◽  
...  
Keyword(s):  
Tumor Cell ◽  
Gene Silencing ◽  
Delivery System ◽  
Self Assembly ◽  
Sirna Delivery ◽  
Single Step ◽  
Chimeric Peptide ◽  
Supramolecular Nanoparticles ◽  
Sirna Delivery System

A practical and tumor cell-specific siRNA delivery system was developedviasingle-step self-assembly of an arginine-rich chimeric peptide with siRNA.

scholarly journals Diblock Copolymer Hydrophobicity Facilitates Efficient Gene Silencing and Cytocompatible Nanoparticle-Mediated siRNA Delivery to Musculoskeletal Cell Types

2017 ◽  
Vol 18 (11) ◽  
pp. 3753-3765 ◽  
Author(s):  
Dominic W. Malcolm ◽  
Margaret A. T. Freeberg ◽  
Yuchen Wang ◽  
Kenneth R. Sims ◽  
Hani A. Awad ◽  
...  
Keyword(s):  
Gene Silencing ◽  
Diblock Copolymer ◽  
Sirna Delivery ◽  
Cell Types ◽  
Efficient Gene

Stimuli-responsive release and efficient siRNA delivery in non-small cell lung cancer by a poly(l-histidine)-based multifunctional nanoplatform

2020 ◽  
Vol 8 (8) ◽  
pp. 1616-1628 ◽  
Author(s):  
Menghao Shi ◽  
Jiulong Zhang ◽  
Ziyuan Huang ◽  
Yuying Chen ◽  
Shuang Pan ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Gene Silencing ◽  
Small Cell Lung Cancer ◽  
Cell Lung Cancer ◽  
Sirna Delivery ◽  
Small Cell ◽  
Proton Sponge ◽  
Stimuli Responsive ◽  
Small Cell Lung

A stimuli-responsive nanoplatform achieves successful intracellular siRNA delivery due to a proton sponge effect based on poly(l-histidine) and effective gene silencing in vivo.

scholarly journals Switching the intracellular pathway and enhancing the therapeutic efficacy of small interfering RNA by auroliposome

2020 ◽  
Vol 6 (30) ◽  
pp. eaba5379 ◽  
Author(s):  
Md. Nazir Hossen ◽  
Lin Wang ◽  
Harisha R. Chinthalapally ◽  
Joe D. Robertson ◽  
Kar-Ming Fung ◽  
...  
Keyword(s):  
Ovarian Cancer ◽  
Gold Nanoparticles ◽  
Gene Silencing ◽  
Small Interfering Rna ◽  
Sirna Delivery ◽  
Delivery Systems ◽  
Ovarian Cancer Cells ◽  
Interfering Rna

Gene silencing using small-interfering RNA (siRNA) is a viable therapeutic approach; however, the lack of effective delivery systems limits its clinical translation. Herein, we doped conventional siRNA-liposomal formulations with gold nanoparticles to create “auroliposomes,” which significantly enhanced gene silencing. We targeted MICU1, a novel glycolytic switch in ovarian cancer, and delivered MICU1-siRNA using three delivery systems—commercial transfection agents, conventional liposomes, and auroliposomes. Low-dose siRNA via transfection or conventional liposomes was ineffective for MICU1 silencing; however, in auroliposomes, the same dose gave >85% gene silencing. Efficacy was evident from both in vitro growth assays of ovarian cancer cells and in vivo tumor growth in human ovarian cell line—and patient-derived xenograft models. Incorporation of gold nanoparticles shifted intracellular uptake pathways such that liposomes avoided degradation within lysosomes. Auroliposomes were nontoxic to vital organs. Therefore, auroliposomes represent a novel siRNA delivery system with superior efficacy for multiple therapeutic applications.

In Vivo Dendritic Cell-specific Gene Silencing Using a Novel and Selective siRNA Delivery Method to Induce Immune Modulation

2007 ◽  
Vol 123 ◽  
pp. S64
Author(s):  
Costin Vladau ◽  
Dong Chen ◽  
Motohiko Suzuki ◽  
Xusheng Zhang ◽  
Xiufen Zheng ◽  
...  
Keyword(s):  
Dendritic Cell ◽  
Gene Silencing ◽  
Immune Modulation ◽  
Sirna Delivery ◽  
Specific Gene ◽  
Delivery Method

scholarly journals Novel fusion peptide‐mediated siRNA delivery using self‐assembled nanocomplex

2021 ◽  
Vol 19 (1) ◽  
Author(s):  
Yeong Chae Ryu ◽  
Kyung Ah Kim ◽  
Byoung Choul Kim ◽  
Hui-Min David Wang ◽  
Byeong Hee Hwang
Keyword(s):  
Gene Silencing ◽  
Cellular Uptake ◽  
Viral Infections ◽  
Immune Cell ◽  
Sirna Delivery ◽  
Cellular Membrane ◽  
Fusion Peptides ◽  
Self Assembled

Abstract Background Gene silencing using siRNA can be a new potent strategy to treat many incurable diseases at the genetic level, including cancer and viral infections. Treatments using siRNA essentially requires an efficient and safe method of delivering siRNA into cells while maintaining its stability. Thus, we designed novel synergistic fusion peptides, i.e., SPACE and oligoarginine. Results Among the novel fusion peptides and siRNAs, nanocomplexes have enhanced cellular uptake and gene silencing effect in vitro and improved retention and gene silencing effects of siRNAs in vivo. Oligoarginine could attract siRNAs electrostatically to form stable and self-assembled nanocomplexes, and the SPACE peptide could interact with the cellular membrane via hydrogen bonding. Therefore, nanocomplexes using fusion peptides showed improved and evident cellular uptake and gene silencing of glyceraldehyde 3-phosphate dehydrogenase (GAPDH) via the lipid raft-mediated endocytosis pathway, especially to the HDFn cells of the skin, and all of the fusion peptides were biocompatible. Also, intratumorally injected nanocomplexes had increased retention time of siRNAs at the site of the tumor. Finally, nanocomplexes demonstrated significant in vivo gene silencing effect without overt tissue damage and immune cell infiltration. Conclusions The new nanocomplex strategy could become a safe and efficient platform for the delivery of siRNAs into cells and tissues to treat various target diseases through gene silencing.

Sign in / Sign up

Export Citation Format

Share Document