In Vivo Dendritic Cell-specific Gene Silencing Using a Novel and Selective siRNA Delivery Method to Induce Immune Modulation

2007 ◽  
Vol 123 ◽  
pp. S64
Author(s):  
Costin Vladau ◽  
Dong Chen ◽  
Motohiko Suzuki ◽  
Xusheng Zhang ◽  
Xiufen Zheng ◽  
...  
Keyword(s):  
Dendritic Cell ◽  
Gene Silencing ◽  
Immune Modulation ◽  
Sirna Delivery ◽  
Specific Gene ◽  
Delivery Method

scholarly journals In vivo gene silencing following non-invasive siRNA delivery into the skin using a novel topical formulation

2014 ◽  
Vol 196 ◽  
pp. 355-362 ◽  
Author(s):  
Vikas Hegde ◽  
Robyn P. Hickerson ◽  
Sitheswaran Nainamalai ◽  
Paul A. Campbell ◽  
Frances J.D. Smith ◽  
...  
Keyword(s):  
Gene Silencing ◽  
Sirna Delivery ◽  
Topical Formulation ◽  
Non Invasive

Stimuli-responsive release and efficient siRNA delivery in non-small cell lung cancer by a poly(l-histidine)-based multifunctional nanoplatform

2020 ◽  
Vol 8 (8) ◽  
pp. 1616-1628 ◽  
Author(s):  
Menghao Shi ◽  
Jiulong Zhang ◽  
Ziyuan Huang ◽  
Yuying Chen ◽  
Shuang Pan ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Gene Silencing ◽  
Small Cell Lung Cancer ◽  
Cell Lung Cancer ◽  
Sirna Delivery ◽  
Small Cell ◽  
Proton Sponge ◽  
Stimuli Responsive ◽  
Small Cell Lung

A stimuli-responsive nanoplatform achieves successful intracellular siRNA delivery due to a proton sponge effect based on poly(l-histidine) and effective gene silencing in vivo.

scholarly journals Switching the intracellular pathway and enhancing the therapeutic efficacy of small interfering RNA by auroliposome

2020 ◽  
Vol 6 (30) ◽  
pp. eaba5379 ◽  
Author(s):  
Md. Nazir Hossen ◽  
Lin Wang ◽  
Harisha R. Chinthalapally ◽  
Joe D. Robertson ◽  
Kar-Ming Fung ◽  
...  
Keyword(s):  
Ovarian Cancer ◽  
Gold Nanoparticles ◽  
Gene Silencing ◽  
Small Interfering Rna ◽  
Sirna Delivery ◽  
Delivery Systems ◽  
Ovarian Cancer Cells ◽  
Interfering Rna

Gene silencing using small-interfering RNA (siRNA) is a viable therapeutic approach; however, the lack of effective delivery systems limits its clinical translation. Herein, we doped conventional siRNA-liposomal formulations with gold nanoparticles to create “auroliposomes,” which significantly enhanced gene silencing. We targeted MICU1, a novel glycolytic switch in ovarian cancer, and delivered MICU1-siRNA using three delivery systems—commercial transfection agents, conventional liposomes, and auroliposomes. Low-dose siRNA via transfection or conventional liposomes was ineffective for MICU1 silencing; however, in auroliposomes, the same dose gave >85% gene silencing. Efficacy was evident from both in vitro growth assays of ovarian cancer cells and in vivo tumor growth in human ovarian cell line—and patient-derived xenograft models. Incorporation of gold nanoparticles shifted intracellular uptake pathways such that liposomes avoided degradation within lysosomes. Auroliposomes were nontoxic to vital organs. Therefore, auroliposomes represent a novel siRNA delivery system with superior efficacy for multiple therapeutic applications.

scholarly journals Novel fusion peptide‐mediated siRNA delivery using self‐assembled nanocomplex

2021 ◽  
Vol 19 (1) ◽  
Author(s):  
Yeong Chae Ryu ◽  
Kyung Ah Kim ◽  
Byoung Choul Kim ◽  
Hui-Min David Wang ◽  
Byeong Hee Hwang
Keyword(s):  
Gene Silencing ◽  
Cellular Uptake ◽  
Viral Infections ◽  
Immune Cell ◽  
Sirna Delivery ◽  
Cellular Membrane ◽  
Fusion Peptides ◽  
Self Assembled

Abstract Background Gene silencing using siRNA can be a new potent strategy to treat many incurable diseases at the genetic level, including cancer and viral infections. Treatments using siRNA essentially requires an efficient and safe method of delivering siRNA into cells while maintaining its stability. Thus, we designed novel synergistic fusion peptides, i.e., SPACE and oligoarginine. Results Among the novel fusion peptides and siRNAs, nanocomplexes have enhanced cellular uptake and gene silencing effect in vitro and improved retention and gene silencing effects of siRNAs in vivo. Oligoarginine could attract siRNAs electrostatically to form stable and self-assembled nanocomplexes, and the SPACE peptide could interact with the cellular membrane via hydrogen bonding. Therefore, nanocomplexes using fusion peptides showed improved and evident cellular uptake and gene silencing of glyceraldehyde 3-phosphate dehydrogenase (GAPDH) via the lipid raft-mediated endocytosis pathway, especially to the HDFn cells of the skin, and all of the fusion peptides were biocompatible. Also, intratumorally injected nanocomplexes had increased retention time of siRNAs at the site of the tumor. Finally, nanocomplexes demonstrated significant in vivo gene silencing effect without overt tissue damage and immune cell infiltration. Conclusions The new nanocomplex strategy could become a safe and efficient platform for the delivery of siRNAs into cells and tissues to treat various target diseases through gene silencing.

In Vivo Myofibroblast Specific Gene Silencing in the Liver Using Novel Sirna-Loaded Biodegradable Nanohydrogel Particles

2016 ◽  
Vol 64 (2) ◽  
pp. S448
Author(s):  
L. Kaps ◽  
L. Nuhn ◽  
M. Aslam ◽  
A. Brose ◽  
F. Foerster ◽  
...  
Keyword(s):  
Gene Silencing ◽  
Specific Gene

scholarly journals Gene silencing following siRNA delivery to skin via coated steel microneedles: In vitro and in vivo proof-of-concept

2013 ◽  
Vol 166 (3) ◽  
pp. 211-219 ◽  
Author(s):  
Rosalind H.E. Chong ◽  
Emilio Gonzalez-Gonzalez ◽  
Maria F. Lara ◽  
Tycho J. Speaker ◽  
Christopher H. Contag ◽  
...  
Keyword(s):  
Gene Silencing ◽  
Sirna Delivery ◽  
Coated Steel ◽  
Proof Of Concept

scholarly journals Engineered nanoparticles for systemic siRNA delivery to malignant brain tumours

Nanoscale ◽  
2019 ◽  
Vol 11 (42) ◽  
pp. 20045-20057 ◽  
Author(s):  
Johan Karlsson ◽  
Yuan Rui ◽  
Kristen L. Kozielski ◽  
Amanda L. Placone ◽  
Olivia Choi ◽  
...  
Keyword(s):  
Mouse Model ◽  
Gene Silencing ◽  
Blood Brain Barrier ◽  
Brain Tumours ◽  
Sirna Delivery ◽  
Engineered Nanoparticles ◽  
Brain Barrier ◽  
Blood Brain ◽  
Malignant Brain Tumours

Bioreducible nanoparticles were engineered for safe and effective systemic siRNA delivery, including crossing the blood–brain barrier to achieve in vivo gene silencing in an orthotopic glioblastoma mouse model.

scholarly journals In vivo trans-specific gene silencing in fungal cells by in planta expression of a double-stranded RNA

BMC Biology ◽  
2010 ◽  
Vol 8 (1) ◽  
pp. 27 ◽  
Author(s):  
Maria Tinoco ◽  
Bárbara BA Dias ◽  
Rebeca C Dall'Astta ◽  
João A Pamphile ◽  
Francisco JL Aragão
Keyword(s):  
Gene Silencing ◽  
Specific Gene ◽  
In Planta ◽  
Double Stranded Rna
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