Rapid and selective identification of molecular species in phosphatidylcholine and sphingomyelin by conditional neutral loss scanning and MS3

2004 ◽  
Vol 18 (24) ◽  
pp. 3123-3130 ◽  
Author(s):  
Toshiaki Houjou ◽  
Kotoko Yamatani ◽  
Hiroki Nakanishi ◽  
Masayoshi Imagawa ◽  
Takao Shimizu ◽  
...  
Keyword(s):  
Molecular Species ◽  
Neutral Loss ◽  
Neutral Loss Scanning

scholarly journals Detection of the abundance of diacylglycerol and triacylglycerol molecular species in cells using neutral loss mass spectrometry

2007 ◽  
Vol 366 (1) ◽  
pp. 59-70 ◽  
Author(s):  
Robert C. Murphy ◽  
Patrick F. James ◽  
Andrew M. McAnoy ◽  
Jessica Krank ◽  
Eva Duchoslav ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Molecular Species ◽  
Neutral Loss ◽  
Triacylglycerol Molecular Species ◽  
In Cells

Lipid Profiling by Multiple Precursor and Neutral Loss Scanning Driven by the Data-Dependent Acquisition

2006 ◽  
Vol 78 (2) ◽  
pp. 585-595 ◽  
Author(s):  
Dominik Schwudke ◽  
Jeffrey Oegema ◽  
Lyle Burton ◽  
Eugeni Entchev ◽  
J. Thomas Hannich ◽  
...  
Keyword(s):  
Neutral Loss ◽  
Lipid Profiling ◽  
Neutral Loss Scanning

Constant neutral loss scanning for the characterization of glycerol phosphatidylcholine phospholipids

1998 ◽  
Vol 9 (11) ◽  
pp. 1189-1195 ◽  
Author(s):  
Pedro Domingues ◽  
Francisco M. L. Amado ◽  
M. Graça O. Santana-Marques ◽  
A. J. Ferrer-Correia
Keyword(s):  
Neutral Loss ◽  
Constant Neutral Loss ◽  
Neutral Loss Scanning

Carboxylic acid functional group analysis using constant neutral loss scanning-mass spectrometry

2007 ◽  
Vol 605 (1) ◽  
pp. 61-69 ◽  
Author(s):  
Julien Dron ◽  
Gregory Eyglunent ◽  
Brice Temime-Roussel ◽  
Nicolas Marchand ◽  
Henri Wortham
Keyword(s):  
Mass Spectrometry ◽  
Carboxylic Acid ◽  
Functional Group ◽  
Neutral Loss ◽  
Group Analysis ◽  
Constant Neutral Loss ◽  
Functional Group Analysis ◽  
Acid Functional Group ◽  
Neutral Loss Scanning

Constant neutral loss scanning for the characterization of bacterial phospholipids desorbed by fast atom bombardment

1988 ◽  
Vol 60 (24) ◽  
pp. 2787-2791 ◽  
Author(s):  
D. N. Heller ◽  
C. M. Murphy ◽  
R. J. Cotter ◽  
C. Fenselau ◽  
O. M. Uy
Keyword(s):  
Fast Atom Bombardment ◽  
Neutral Loss ◽  
Constant Neutral Loss ◽  
Neutral Loss Scanning

Detection and identification of Catalpol metabolites in the rat plasma, urine and faeces using ultra-high performance liquid chromatography-Q Exactive hybrid quadrupole-orbitrap high-resolution accurate mass spectrometry

2020 ◽  
Vol 21 ◽  
Author(s):  
Zedong Xiang ◽  
Shaoping Wang ◽  
Haoran Li ◽  
Pingping Dong ◽  
Fan Dong ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
High Resolution ◽  
High Performance ◽  
Mass Measurement ◽  
Neutral Loss ◽  
Rat Plasma ◽  
Accurate Mass ◽  
Chromatographic Retention ◽  
Rat Urine ◽  
Q Exactive

Background:: Catalpol, an iridoid glycoside, is one of the richest bioactive components present in Rehmannia glutinosa. More and more metabolites of drugs have exhibit various pharmacological effects, thus providing guidance for clinical application. However, few researches have paid attention on the metabolism of catalpol. Objective:: This study aimed to establish a rapid and effective method to identify catalpol metabolites and evaluate the biotransformation pathways of catalpol in rats. Methods:: In this study, catalpol metabolites in rat urine, plasma and faeces were analyzed by UHPLC-Q-Exactive MS for the characterization of metabolism of catalpol. Based on high-resolution extracted ion chromatograms (HREICs) and parallel reaction monitoring mode (PRM), metabolites of catalpol were identified by comparing the diagnostic product ions (DPIs), chromatographic retention times, neutral loss fragments (NLFs) and accurate mass measurement with those of catalpol reference standard. Results: A total of 29 catalpol metabolites were detected and identified in both negative and positive ion modes. Nine metabolic reactions including deglycosylation, hydroxylation, dihydroxylation, hydrogenation, dehydrogenation, oxidation of methylene to ketone, glucuronidation, glycine conjugation and cysteine conjugation were proposed. Conclusion:: A rapid and effective method based on UHPLC-Q-Exactive MS was developed to mine the metabolism information of catalpol. Results of metabolites and biotransformation pathways of catalpol suggested that when orally administrated, catalpol was firstly metabolized into catalpol aglycone, after which phase Ⅰ and phase Ⅱ reactions occurred. However, hydrophilic chromatography-mass spectrometry still needed to further find the polar metabolites of catalpol.

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