Evaluation of urinary ribonucleoside profiling for clinical biomarker discovery using constant neutral loss scanning liquid chromatography/tandem mass spectrometry

2011 ◽  
Vol 25 (14) ◽  
pp. 2071-2082 ◽  
Author(s):  
Friederike Teichert ◽  
Swantje Winkler ◽  
Hector C. Keun ◽  
William P. Steward ◽  
Andreas J. Gescher ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Biomarker Discovery ◽  
Neutral Loss ◽  
Tandem Mass ◽  
Chromatography Tandem Mass Spectrometry ◽  
Constant Neutral Loss ◽  
Liquid Chromatography Tandem Mass ◽  
Clinical Biomarker ◽  
Neutral Loss Scanning

scholarly journals LC-MS/MS Method Development for the Discovery and Identification of Amphidinols Produced by Amphidinium

Marine Drugs ◽  
2020 ◽  
Vol 18 (10) ◽  
pp. 497
Author(s):  
Marvin Wellkamp ◽  
Francisco García-Camacho ◽  
Lorena M. Durán-Riveroll ◽  
Jan Tebben ◽  
Urban Tillmann ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Method Development ◽  
Neutral Loss ◽  
Tandem Mass ◽  
Reaction Monitoring ◽  
Fish Kills ◽  
Chromatography Tandem Mass Spectrometry ◽  
Liquid Chromatography Tandem Mass ◽  
Fragmentation Patterns

Amphidinols are polyketides produced by dinoflagellates suspected of causing fish kills. Here, we demonstrate a liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for the identification and quantification of amphidinols (AM). Novel AM were detected by neutral loss (NL) scan and then quantified together with known AM by selection reaction monitoring (SRM). With the new method, AM were detected in four of eight analyzed strains with a maximum of 3680 fg toxin content per cell. In total, sixteen novel AM were detected by NL scan and characterized via their fragmentation patterns. Of these, two substances are glycosylated forms. This is the first detection of glycosylated AM.

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