DNA macroarray study of skin aging-related genes expression modulation by antioxidant plant extracts on a replicative senescence model of human dermal fibroblasts

2010 ◽  
Vol 25 (5) ◽  
pp. 686-693 ◽  
Author(s):  
Stéphanie Dudonné ◽  
Philippe Coutière ◽  
Marion Woillez ◽  
Jean-Michel Mérillon ◽  
Xavier Vitrac
Keyword(s):  
Plant Extracts ◽  
Replicative Senescence ◽  
Skin Aging ◽  
Dermal Fibroblasts ◽  
Human Dermal Fibroblasts ◽  
Genes Expression ◽  
Expression Modulation

Photobiomodulation with 590 nm Wavelength Delays the Telomere Shortening and Replicative Senescence of Human Dermal Fibroblasts In Vitro

2020 ◽  
Vol 38 (11) ◽  
pp. 656-660
Author(s):  
Borislav Arabadjiev ◽  
Roumen Pankov ◽  
Ivelina Vassileva ◽  
Lyuben Sashov Petrov ◽  
Ivan Buchvarov
Keyword(s):  
Replicative Senescence ◽  
Dermal Fibroblasts ◽  
Human Dermal Fibroblasts ◽  
Telomere Shortening

scholarly journals Preventive Effects of Epigallocatechin-3-O-Gallate against Replicative Senescence Associated with p53 Acetylation in Human Dermal Fibroblasts

2012 ◽  
Vol 2012 ◽  
pp. 1-13 ◽  
Author(s):  
Dong-Wook Han ◽  
Mi Hee Lee ◽  
Bongju Kim ◽  
Jun Jae Lee ◽  
Suong-Hyu Hyon ◽  
...  
Keyword(s):  
Cell Cycle Progression ◽  
Replicative Senescence ◽  
Nuclear Translocation ◽  
Articular Chondrocytes ◽  
Underlying Mechanism ◽  
Serial Passage ◽  
Dermal Fibroblasts ◽  
Human Dermal Fibroblasts ◽  
Primary Cells ◽  
Preventive Effects

Considering the various pharmacological activities of epigallocatechin-3-O-gallate (EGCG) including anticancer, and anti-inflammatory, antidiabetic, and so forth, relatively less attention has been paid to the antiaging effect of EGCG on primary cells. In this study, the preventive effects of EGCG against serial passage-induced senescence were investigated in primary cells including rat vascular smooth muscle cells (RVSMCs), human dermal fibroblasts (HDFs), and human articular chondrocytes (HACs). The involvement of Sirt1 and acetylated p53 was examined as an underlying mechanism for the senescence preventive activity of EGCG in HDFs. All cells were employed with the initial passage number (PN) between 3 and 7. For inducing senescence, the cells were serially passaged at the predetermined times and intervals in the absence or presence of EGCG (50 or 100 μM). Serial passage-induced senescence in RVSMCs and HACs was able to be significantly prevented at 50 μM EGCG, while in HDFs, 100 μM EGCG could significantly prevent senescence and recover their cell cycle progression close to the normal level. Furthermore, EGCG was found to prevent serial passage- and H2O2-induced senescence in HDFs by suppressing p53 acetylation, but the Sirt1 activity was unaffected. In addition, proliferating HDFs showed similar cellular uptake of FITC-conjugated EGCG into the cytoplasm with their senescent counterparts but different nuclear translocation of it from them, which would partly account for the differential responses to EGCG in proliferating versus senescent cells. Taking these results into consideration, it is suggested that EGCG may be exploited to craft strategies for the development of an antiaging or age-delaying agent.

scholarly journals (−)-Loliolide Isolated from Sargassum horneri Abate UVB-Induced Oxidative Damage in Human Dermal Fibroblasts and Subside ECM Degradation

Marine Drugs ◽  
2021 ◽  
Vol 19 (8) ◽  
pp. 435
Author(s):  
Ilekuttige Priyan Shanura Fernando ◽  
Soo-Jin Heo ◽  
Mawalle Kankanamge Hasitha Madhawa Dias ◽  
Dissanayaka Mudiyanselage Dinesh Madusanka ◽  
Eui-Jeong Han ◽  
...  
Keyword(s):  
Inflammatory Responses ◽  
Skin Aging ◽  
Dermal Fibroblasts ◽  
Sargassum Horneri ◽  
Mitogen Activated Protein Kinase ◽  
Ros Generation ◽  
Protective Effects ◽  
Human Dermal Fibroblasts ◽  
Mitogen Activated Protein ◽  
Uvb Exposure

Ultraviolet (UV) B exposure is a prominent cause of skin aging and a contemporary subject of interest. The effects are progressing through the generation of reactive oxygen species (ROS) that alter cell signaling pathways related to inflammatory responses. The present study evaluates the protective effects of (7aR)-6-hydroxy-4,4,7a-trimethyl-6,7-dihydro-5H-1-benzofuran-2-one (HTT) isolated from the edible brown algae Sargassum horneri against UVB protective effects in human dermal fibroblasts (HDFs). HTT treatment dose-dependently suppressed intracellular ROS generation in HDFs with an IC50 of 62.43 ± 3.22 µM. HTT abated UVB-induced mitochondrial hyperpolarization and apoptotic body formation. Furthermore, UVB-induced activation of key nuclear factor (NF)-κB and mitogen-activated protein kinase signaling proteins were suppressed in HTT treated cells while downregulating pro-inflammatory cytokines (interleukin-1β, 6, 8, 33 and tumor necrosis factor-α). Moreover, HTT treatment downregulated matrix metalloproteinase1, 2, 3, 8, 9 and 13 that was further confirmed by the inhibition of collagenase and elastase activity. The evidence implies that HTT delivers protective effects against premature skin aging caused by UVB exposure via suppressing inflammatory responses and degradation of extracellular matrix (ECM) components. Extensive research in this regard will raise perspectives for using HTT as an ingredient in UV protective ointments.

Replicative senescence of human dermal fibroblasts affects structural and functional aspects of the Golgi apparatus

2019 ◽  
Vol 28 (8) ◽  
pp. 922-932 ◽  
Author(s):  
Julie Despres ◽  
Yasmina Ramdani ◽  
Marine di Giovanni ◽  
Magalie Bénard ◽  
Abderrakib Zahid ◽  
...  
Keyword(s):  
Golgi Apparatus ◽  
Replicative Senescence ◽  
Dermal Fibroblasts ◽  
Human Dermal Fibroblasts ◽  
Functional Aspects

scholarly journals Anti-Thermal Skin Aging Activity of Aqueous Extracts Derived from Apple Mint (Mentha suaveolens Ehrh.) in Human Dermal Fibroblasts

2018 ◽  
Vol 2018 ◽  
pp. 1-7 ◽  
Author(s):  
Dahee Son ◽  
Minkyung Kim ◽  
Hyunju Woo ◽  
Deokhoon Park ◽  
Eunsun Jung
Keyword(s):  
Heat Shock ◽  
Antioxidant Activities ◽  
Folk Medicine ◽  
Skin Aging ◽  
Dermal Fibroblasts ◽  
Shock Treatment ◽  
Human Dermal Fibroblasts ◽  
Heat Shock Treatment ◽  
Biological Assays ◽  
Extracellular Signal

Thermal skin aging refers to skin aging induced by heat shock treatment. Apple mint (Mentha suaveolens Ehrh.) has been used as a folk medicine to treat various diseases. However, the activity of apple mint in thermal skin aging has yet to be investigated. In this study, we conducted various biological assays to demonstrate the anti-thermal skin aging activity of extracts of apple mint leaves (ALE). As a result, ALE showed significant antioxidant activities and inhibited the production of reactive oxygen species (ROS), matrix metalloproteinases (MMPs), and interleukin-8 (IL-8) as well as suppressed mitogen-activated proteins kinases (MAPKs) such as extracellular signal regulated kinases (ERK), c-Jun N terminal kinases (JNK), and p38 MAPK triggered by heat shock treatment in human dermal fibroblasts (HDFs). Consequently, ALE could be used as attractive cosmetic materials with anti-thermal skin aging activity.

scholarly journals Chitosan hydrogel-loaded MSC-derived extracellular vesicles promote skin rejuvenation by ameliorating the senescence of dermal fibroblasts

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Xiangnan Zhao ◽  
Yue Liu ◽  
Pingping Jia ◽  
Hui Cheng ◽  
Chen Wang ◽  
...  
Keyword(s):  
Extracellular Vesicles ◽  
Subcutaneous Injection ◽  
Replicative Senescence ◽  
Skin Aging ◽  
Dermal Fibroblasts ◽  
Related Factors ◽  
Ecm Proteins ◽  
Aging Skin

Abstract Background The senescence of dermal fibroblasts (DFLs) leads to an imbalance in the synthesis and degradation of extracellular matrix (ECM) proteins, presenting so-called senescence-associated secretory phenotype (SASP), which ultimately leads to skin aging. Recently, mesenchymal stem cell (MSC)-derived extracellular vesicles (EVs) have been recognized as a promising cell-free therapy for degenerative diseases, which opens a new avenue for skin aging treatment. Methods In this study, we utilized chitosan (CS) hydrogel for effective loading and sustained release of EVs. In vitro, we explored the rejuvenation effects of CS hydrogel-incorporated EVs (CS-EVs) on replicative senescence DFLs through a series of experiments such as senescence-associated β-galactosidase (SA-β-gal) staining, RT-PCR, and Western blot analysis. Besides, we employed local multi-site subcutaneous injection to treat skin aging of naturally aged mice with CS-EVs and DiI fluorescent dye was used to label EVs to achieve in vivo real-time tracking. Results CS-EVs can significantly improve the biological functions of senescent fibroblasts, including promoting their proliferation, enhancing the synthesis of ECM proteins, and inhibiting the overexpression of matrix metalloproteinases (MMPs). Moreover, CS hydrogel could prolong the release of EVs and significantly increase the retention of EVs in vivo. After CS-EVs subcutaneous injection treatment, the aging skin tissues showed a rejuvenation state, manifested explicitly as the enhanced expression of collagen, the decreased expression of SASP-related factors, and the restoration of tissue structures. Conclusions CS hydrogel-encapsulated EVs could delay the skin aging processes by ameliorating the function of aging DFLs. Our results also highlight the potential of CS hydrogel-encapsulated EVs as a novel therapeutic strategy for improving aging skin to rejuvenation.

scholarly journals Heat Treatment Improves UV Photoprotective Effects of Licorice in Human Dermal Fibroblasts

Processes ◽  
2021 ◽  
Vol 9 (6) ◽  
pp. 1040
Author(s):  
Jeong-Yong Park ◽  
Yun-Jeong Ji ◽  
Kyung Hye Seo ◽  
Ji Yeon Lee ◽  
Geum-Soog Kim ◽  
...  
Keyword(s):  
Heat Treatment ◽  
Skin Aging ◽  
Dermal Fibroblasts ◽  
Ultraviolet B ◽  
Human Dermal Fibroblasts ◽  
Uvb Radiation ◽  
External Stimulation ◽  
Heat Treated ◽  
Licorice Extract ◽  
Anti Inflammatory

External stimulation of the skin by ultraviolet B (UVB) radiation induces oxidative stress or inflammation, causing skin aging and skin cancer. Glycyrrhiza uralensis (licorice) has been used as a medicinal plant for its antioxidant, anti-inflammatory, antiviral, antimicrobial, anticarcinogenic, and hepatoprotective properties. The present study analyzed the effects of thermal processing on the bioactivities of licorice. Heat-treated licorice (HL) extracts had better antioxidant and anti-inflammatory activities than nontreated licorice (NL) extract. HL extracts also had higher total phenol contents than NL extract. In particular, contents of isoliquiritigenin, an antioxidant and anti-inflammatory substance of licorice, increased in proportion to the skin-protection effects of HL extracts. Heat treatment increased the contents of phenolic compounds such as isoliquiritigenin in licorice extract, which improved the UV photoprotective effect of licorice in human dermal fibroblasts.

scholarly journals Skin Aging-Dependent Activation of the PI3K Signaling Pathway via Downregulation of PTEN Increases Intracellular ROS in Human Dermal Fibroblasts

2016 ◽  
Vol 2016 ◽  
pp. 1-9 ◽  
Author(s):  
Eun-Mi Noh ◽  
Jinny Park ◽  
Hwa-Ryung Song ◽  
Jeong-Mi Kim ◽  
Minok Lee ◽  
...  
Keyword(s):  
Nadph Oxidase ◽  
Signaling Pathway ◽  
Skin Aging ◽  
Dermal Fibroblasts ◽  
Ros Generation ◽  
Human Dermal Fibroblasts ◽  
Chronological Aging ◽  
Aged Skin ◽  
Pi3k Signaling Pathway ◽  
High Level

Reactive oxygen species (ROS) play a major role in both chronological aging and photoaging. ROS induce skin aging through their damaging effect on cellular constituents. However, the origins of ROS have not been fully elucidated. We investigated that ROS generation of replicative senescent fibroblasts is generated by the modulation of phosphatidylinositol 3,4,5-triphosphate (PIP3) metabolism. Reduction of the PTEN protein, which dephosphorylates PIP3, was responsible for maintaining a high level of PIP3 in replicative cells and consequently mediated the activation of the phosphatidylinositol-3-OH kinase (PI3K)/Akt pathway. Increased ROS production was blocked by inhibition of PI3K or protein kinase C (PKC) or by NADPH oxidase activating in replicative senescent cells. These data indicate that the signal pathway to ROS generation in replicative aged skin cells can be stimulated by reduced PTEN level. Our results provide new insights into skin aging-associated modification of the PI3K/NADPH oxidase signaling pathway and its relationship with a skin aging-dependent increase of ROS in human dermal fibroblasts.

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