Poria cocos (Fuling) targets TGFβ /Smad7 associated collagen accumulation and enhances Nrf2‐antioxidant mechanism to exert anti‐skin aging effects in human dermal fibroblasts

2020 ◽  
Author(s):  
Chien‐Liang Fang ◽  
Catherine Reena Paul ◽  
Cecilia Hsuan Day ◽  
Ruey‐Lin Chang ◽  
Chia‐Hua Kuo ◽  
...  
Keyword(s):  
Skin Aging ◽  
Dermal Fibroblasts ◽  
Human Dermal Fibroblasts ◽  
Aging Effects ◽  
Poria Cocos ◽  
Antioxidant Mechanism ◽  
Collagen Accumulation

scholarly journals Anti-oxidant and Anti-skin-aging Effects of Abalone Viscera Extracts in Human Dermal Fibroblasts

2012 ◽  
Vol 19 (4) ◽  
pp. 463-469 ◽  
Author(s):  
Jinglei Li ◽  
Tao Tong ◽  
Du-Ock Ko ◽  
Dong-Ok Chung ◽  
Won-Chul Jeong ◽  
...  
Keyword(s):  
Skin Aging ◽  
Dermal Fibroblasts ◽  
Human Dermal Fibroblasts ◽  
Aging Effects ◽  
Anti Oxidant

scholarly journals A 1,1′-biuracil from Epidermidibacterium keratini EPI-7 shows anti-aging effects on human dermal fibroblasts

2019 ◽  
Vol 62 (1) ◽  
Author(s):  
Yeong-Geun Lee ◽  
Dong-Geol Lee ◽  
Jung Eun Gwag ◽  
Misun Kim ◽  
Minji Kim ◽  
...  
Keyword(s):  
Dermal Fibroblasts ◽  
Human Dermal Fibroblasts ◽  
Aging Effects

scholarly journals Synthesis of Kisspeptin-Mimicking Fragments and Investigation of their Skin Anti-Aging Effects

2020 ◽  
Vol 21 (22) ◽  
pp. 8439
Author(s):  
Kyung-Eun Lee ◽  
Sugyeong Jeong ◽  
Seok Kyun Yun ◽  
Seoyeon Kyung ◽  
Abadie Sophie ◽  
...  
Keyword(s):  
Skin Aging ◽  
Dermal Fibroblasts ◽  
Biologically Active ◽  
Type I ◽  
Aging Effects ◽  
Type I Procollagen ◽  
Reproductive Axis ◽  
Stress Related Genes ◽  
Active Fragments ◽  
Aging Models

In recent years, a number of active materials have been developed to provide anti-aging benefits for skin and, among them, peptides have been considered the most promising candidate due to their remarkable and long-lasting anti-wrinkle activity. Recent studies have begun to elucidate the relationship between the secretion of emotion-related hormones and skin aging. Kisspeptin, a neuropeptide encoded by the KISS1 gene, has gained attention in reproductive endocrinology since it stimulates the reproductive axis in the hypothalamus; however, the effects of Kisspeptin on skin have not been studied yet. In this study, we synthesized Kisspeptin-10 and Kisspeptin-E, which are biologically active fragments, to mimic the action of Kisspeptin. Next, we demonstrated the anti-aging effects of the Kisspeptin-mimicking fragments using UV-induced skin aging models, such as UV-induced human dermal fibroblasts (Hs68) and human skin explants. Kisspeptin-E suppressed UV-induced 11 beta-hydroxysteroid dehydrogenase type 1 (11β-HSD1) stimulation leading to a regulation of skin aging related genes, including type I procollagen, matrix metalloproteinases-1 (MMP-1), interleukin-6 (IL-6), and IL-8, and rescued the skin integrity. Taken together, these results suggest that Kisspeptin-E could be useful to improve UV-induced skin aging by modulating expression of stress related genes, such as 11β-HSD1.

scholarly journals Propolis Suppresses UV-Induced Photoaging in Human Skin through Directly Targeting Phosphoinositide 3-Kinase

Nutrients ◽  
2020 ◽  
Vol 12 (12) ◽  
pp. 3790
Author(s):  
Da Hyun Kim ◽  
Joong-Hyuck Auh ◽  
Jeongyeon Oh ◽  
Seungpyo Hong ◽  
Sungbin Choi ◽  
...  
Keyword(s):  
Protein Kinase ◽  
Human Skin ◽  
Therapeutic Potential ◽  
Skin Aging ◽  
Dermal Fibroblasts ◽  
Kinase Assay ◽  
Aging Effects ◽  
Propolis Extract ◽  
Pi3k Activity ◽  
Phosphoinositide 3 Kinase

Propolis is a resinous substance generated by bees using materials from various plant sources. It has been known to exhibit diverse bioactivities including anti-oxidative, anti-microbial, anti-inflammatory, and anti-cancer effects. However, the direct molecular target of propolis and its therapeutic potential against skin aging in humans is not fully understood. Herein, we investigated the effect of propolis on ultraviolet (UV)-mediated skin aging and its underlying molecular mechanism. Propolis suppressed UV-induced matrix metalloproteinase (MMP)-1 production in human dermal fibroblasts. More importantly, propolis treatment reduced UV-induced MMP-1 expression and blocked collagen degradation in human skin tissues, suggesting that the anti-skin-aging activity of propolis can be recapitulated in clinically relevant conditions. While propolis treatment did not display any noticeable effects against extracellular signal-regulated kinase (ERK), p38, and c-jun N-terminal kinase (JNK) pathways, propolis exerted significant inhibitory activity specifically against phosphorylations of phosphoinositide-dependent protein kinase-1 (PDK1) and protein kinase B (Akt). Kinase assay results demonstrated that propolis can directly suppress phosphoinositide 3-kinase (PI3K) activity, with preferential selectivity towards PI3K with p110α and p110δ catalytic subunits over other kinases. The content of active compounds was quantified, and among the compounds identified from the propolis extract, caffeic acid phenethyl ester, quercetin, and apigenin were shown to attenuate PI3K activity. These results demonstrate that propolis shows anti-skin-aging effects through direct inhibition of PI3K activity.

scholarly journals (−)-Loliolide Isolated from Sargassum horneri Abate UVB-Induced Oxidative Damage in Human Dermal Fibroblasts and Subside ECM Degradation

Marine Drugs ◽  
2021 ◽  
Vol 19 (8) ◽  
pp. 435
Author(s):  
Ilekuttige Priyan Shanura Fernando ◽  
Soo-Jin Heo ◽  
Mawalle Kankanamge Hasitha Madhawa Dias ◽  
Dissanayaka Mudiyanselage Dinesh Madusanka ◽  
Eui-Jeong Han ◽  
...  
Keyword(s):  
Inflammatory Responses ◽  
Skin Aging ◽  
Dermal Fibroblasts ◽  
Sargassum Horneri ◽  
Mitogen Activated Protein Kinase ◽  
Ros Generation ◽  
Protective Effects ◽  
Human Dermal Fibroblasts ◽  
Mitogen Activated Protein ◽  
Uvb Exposure

Ultraviolet (UV) B exposure is a prominent cause of skin aging and a contemporary subject of interest. The effects are progressing through the generation of reactive oxygen species (ROS) that alter cell signaling pathways related to inflammatory responses. The present study evaluates the protective effects of (7aR)-6-hydroxy-4,4,7a-trimethyl-6,7-dihydro-5H-1-benzofuran-2-one (HTT) isolated from the edible brown algae Sargassum horneri against UVB protective effects in human dermal fibroblasts (HDFs). HTT treatment dose-dependently suppressed intracellular ROS generation in HDFs with an IC50 of 62.43 ± 3.22 µM. HTT abated UVB-induced mitochondrial hyperpolarization and apoptotic body formation. Furthermore, UVB-induced activation of key nuclear factor (NF)-κB and mitogen-activated protein kinase signaling proteins were suppressed in HTT treated cells while downregulating pro-inflammatory cytokines (interleukin-1β, 6, 8, 33 and tumor necrosis factor-α). Moreover, HTT treatment downregulated matrix metalloproteinase1, 2, 3, 8, 9 and 13 that was further confirmed by the inhibition of collagenase and elastase activity. The evidence implies that HTT delivers protective effects against premature skin aging caused by UVB exposure via suppressing inflammatory responses and degradation of extracellular matrix (ECM) components. Extensive research in this regard will raise perspectives for using HTT as an ingredient in UV protective ointments.

scholarly journals Anti-Aging Effects of Gyrophoric Acid on UVA-Irradiated Normal Human Dermal Fibroblasts

2020 ◽  
Vol 15 (4) ◽  
pp. 1934578X2091954
Author(s):  
Joong Hyun Shim
Keyword(s):  
Mrna Expression ◽  
Type I Collagen ◽  
Dermal Fibroblasts ◽  
Type I ◽  
Human Dermal Fibroblasts ◽  
Aging Effects ◽  
Expression Levels ◽  
Normal Human ◽  
Normal Human Dermal Fibroblasts ◽  
Gyrophoric Acid

This research was conducted to identify the anti-aging effects of gyrophoric acid on the skin, using normal human dermal fibroblasts. The anti-aging effects of gyrophoric acid on dermal fibroblasts were demonstrated through cell viability, verification of collagen, type I, alpha 1 (COL1A1)/COL3A1/matrix metalloproteinases 1 (MMP1) messenger ribonucleic acid (mRNA) expression levels with quantitative real-time reverse-transcription polymerase chain reaction, and protein estimation using type I collagen/MMP1-enzyme-linked immunosorbent assay. Further, the effects of gyrophoric acid on superoxide dismutases (SODs)/catalase were investigated by assessing their mRNA expression. In ultraviolet A (UVA)-treated dermal fibroblasts, gyrophoric acid was observed to increase mRNA levels of COL1A1/COL3A1/SOD2 genes and type I collagen protein levels, consistent with its anti-aging role. Furthermore, gyrophoric acid treatment decreased both MMP1 mRNA and protein expression levels. Therefore, the results of this study demonstrate that gyrophoric acid can be considered as an important natural compound with potent anti-aging effects on the skin. Based on the findings of this study, further research about the mechanism of action of gyrophoric acid should be pursued so as to develop novel anti-aging strategies not only in the field of cosmetics but also for healthcare.

scholarly journals Rapamycin Protects Skin Fibroblasts From UVA-Induced Photoaging by Inhibition of p53 and Phosphorylated HSP27

2021 ◽  
Vol 9 ◽  
Author(s):  
Gen-Long Bai ◽  
Ping Wang ◽  
Xin Huang ◽  
Zi-Yue Wang ◽  
Di Cao ◽  
...  
Keyword(s):  
Type I Collagen ◽  
Skin Aging ◽  
Dermal Fibroblasts ◽  
Type I ◽  
Aging Effects ◽  
Smad Signaling Pathway ◽  
Skin Photoaging ◽  
Dermal Collagen ◽  
Induced Apoptosis

Skin aging caused by UV radiation is called photoaging is characterized by skin roughness and dryness accompanied by a significant reduction of dermal collagen. Rapamycin is a macrolide immunosuppressant which has been shown to exhibit “anti-aging” effects in cells and organisms, however, its roles in the skin photoaging remains unclear. Here, we investigate the role of rapamycin and HSP27, which we have previously identified as an inhibitor of UV-induced apoptosis and senescence in HaCat cells, in a UVA-induced photoaging model of primary human dermal fibroblasts (HDFs). Results from senescence-associated beta-galactosidase (SA-β-gal) staining revealed that rapamycin significantly reduced senescence in UVA-treated HDFs. In addition, treatment with rapamycin significantly increased cell autophagy levels, decreased the expression of p53 and phosphorylated HSP27, and reduced genotoxic and oxidative cellular stress levels in UVA-induced HDFs. Knockdown of HSP27 resulted in a significant increase of MMP-1 and MMP-3 as well as a decrease in type I collagen expression. Rapamycin mitigated these effects by activation of the classical TGF-β/Smad signaling pathway and increasing the transcriptional activity of MAPK/AP-1. Taken together, these results suggest that rapamycin may potentially serve as a preventive and therapeutic agent for UVA-induced photoaging of the skin.

scholarly journals Anti-Thermal Skin Aging Activity of Aqueous Extracts Derived from Apple Mint (Mentha suaveolens Ehrh.) in Human Dermal Fibroblasts

2018 ◽  
Vol 2018 ◽  
pp. 1-7 ◽  
Author(s):  
Dahee Son ◽  
Minkyung Kim ◽  
Hyunju Woo ◽  
Deokhoon Park ◽  
Eunsun Jung
Keyword(s):  
Heat Shock ◽  
Antioxidant Activities ◽  
Folk Medicine ◽  
Skin Aging ◽  
Dermal Fibroblasts ◽  
Shock Treatment ◽  
Human Dermal Fibroblasts ◽  
Heat Shock Treatment ◽  
Biological Assays ◽  
Extracellular Signal

Thermal skin aging refers to skin aging induced by heat shock treatment. Apple mint (Mentha suaveolens Ehrh.) has been used as a folk medicine to treat various diseases. However, the activity of apple mint in thermal skin aging has yet to be investigated. In this study, we conducted various biological assays to demonstrate the anti-thermal skin aging activity of extracts of apple mint leaves (ALE). As a result, ALE showed significant antioxidant activities and inhibited the production of reactive oxygen species (ROS), matrix metalloproteinases (MMPs), and interleukin-8 (IL-8) as well as suppressed mitogen-activated proteins kinases (MAPKs) such as extracellular signal regulated kinases (ERK), c-Jun N terminal kinases (JNK), and p38 MAPK triggered by heat shock treatment in human dermal fibroblasts (HDFs). Consequently, ALE could be used as attractive cosmetic materials with anti-thermal skin aging activity.

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