Replicative senescence of human dermal fibroblasts affects structural and functional aspects of the Golgi apparatus

2019 ◽  
Vol 28 (8) ◽  
pp. 922-932 ◽  
Author(s):  
Julie Despres ◽  
Yasmina Ramdani ◽  
Marine di Giovanni ◽  
Magalie Bénard ◽  
Abderrakib Zahid ◽  
...  
Keyword(s):  
Golgi Apparatus ◽  
Replicative Senescence ◽  
Dermal Fibroblasts ◽  
Human Dermal Fibroblasts ◽  
Functional Aspects

Photobiomodulation with 590 nm Wavelength Delays the Telomere Shortening and Replicative Senescence of Human Dermal Fibroblasts In Vitro

2020 ◽  
Vol 38 (11) ◽  
pp. 656-660
Author(s):  
Borislav Arabadjiev ◽  
Roumen Pankov ◽  
Ivelina Vassileva ◽  
Lyuben Sashov Petrov ◽  
Ivan Buchvarov
Keyword(s):  
Replicative Senescence ◽  
Dermal Fibroblasts ◽  
Human Dermal Fibroblasts ◽  
Telomere Shortening

scholarly journals Preventive Effects of Epigallocatechin-3-O-Gallate against Replicative Senescence Associated with p53 Acetylation in Human Dermal Fibroblasts

2012 ◽  
Vol 2012 ◽  
pp. 1-13 ◽  
Author(s):  
Dong-Wook Han ◽  
Mi Hee Lee ◽  
Bongju Kim ◽  
Jun Jae Lee ◽  
Suong-Hyu Hyon ◽  
...  
Keyword(s):  
Cell Cycle Progression ◽  
Replicative Senescence ◽  
Nuclear Translocation ◽  
Articular Chondrocytes ◽  
Underlying Mechanism ◽  
Serial Passage ◽  
Dermal Fibroblasts ◽  
Human Dermal Fibroblasts ◽  
Primary Cells ◽  
Preventive Effects

Considering the various pharmacological activities of epigallocatechin-3-O-gallate (EGCG) including anticancer, and anti-inflammatory, antidiabetic, and so forth, relatively less attention has been paid to the antiaging effect of EGCG on primary cells. In this study, the preventive effects of EGCG against serial passage-induced senescence were investigated in primary cells including rat vascular smooth muscle cells (RVSMCs), human dermal fibroblasts (HDFs), and human articular chondrocytes (HACs). The involvement of Sirt1 and acetylated p53 was examined as an underlying mechanism for the senescence preventive activity of EGCG in HDFs. All cells were employed with the initial passage number (PN) between 3 and 7. For inducing senescence, the cells were serially passaged at the predetermined times and intervals in the absence or presence of EGCG (50 or 100 μM). Serial passage-induced senescence in RVSMCs and HACs was able to be significantly prevented at 50 μM EGCG, while in HDFs, 100 μM EGCG could significantly prevent senescence and recover their cell cycle progression close to the normal level. Furthermore, EGCG was found to prevent serial passage- and H2O2-induced senescence in HDFs by suppressing p53 acetylation, but the Sirt1 activity was unaffected. In addition, proliferating HDFs showed similar cellular uptake of FITC-conjugated EGCG into the cytoplasm with their senescent counterparts but different nuclear translocation of it from them, which would partly account for the differential responses to EGCG in proliferating versus senescent cells. Taking these results into consideration, it is suggested that EGCG may be exploited to craft strategies for the development of an antiaging or age-delaying agent.

scholarly journals PTEN inhibits replicative senescence-induced MMP-1 expression by regulating NOX4-mediated ROS in human dermal fibroblasts

2017 ◽  
Vol 21 (11) ◽  
pp. 3113-3116 ◽  
Author(s):  
Eun-Mi Noh ◽  
Jeong-Mi Kim ◽  
On-Yu Hong ◽  
Hyun-Kyung Song ◽  
Jong-Suk Kim ◽  
...  
Keyword(s):  
Replicative Senescence ◽  
Dermal Fibroblasts ◽  
Human Dermal Fibroblasts

Exploration of replicative senescence-associated genes in human dermal fibroblasts by cDNA microarray technology

2004 ◽  
Vol 39 (9) ◽  
pp. 1369-1378 ◽  
Author(s):  
In Kyung Yoon ◽  
Hyun Kyoung Kim ◽  
Yu Kyoung Kim ◽  
In-Hwan Song ◽  
Wankee Kim ◽  
...  
Keyword(s):  
Cdna Microarray ◽  
Replicative Senescence ◽  
Dermal Fibroblasts ◽  
Human Dermal Fibroblasts ◽  
Microarray Technology ◽  
Senescence Associated Genes
Sign in / Sign up

Export Citation Format

Share Document