Crystal structure of the tRNA-specific adenosine deaminase from Streptococcus pyogenes

2007 ◽  
Vol 68 (4) ◽  
pp. 1016-1019 ◽  
Author(s):  
Won-Ho Lee ◽  
Young Kwan Kim ◽  
Ki Hyun Nam ◽  
Amit Priyadarshi ◽  
Eun Hye Lee ◽  
...  
Keyword(s):  
Crystal Structure ◽  
Adenosine Deaminase ◽  
Streptococcus Pyogenes

scholarly journals Crystal structure of a phosphotransacetylase from Streptococcus pyogenes

2004 ◽  
Vol 55 (2) ◽  
pp. 479-481 ◽  
Author(s):  
Qian Steven Xu ◽  
Dong-Hae Shin ◽  
Ramona Pufan ◽  
Hisao Yokota ◽  
Rosalind Kim ◽  
...  
Keyword(s):  
Crystal Structure ◽  
Streptococcus Pyogenes

scholarly journals Crystal Structure of Streptococcus pyogenes Cas1 and Its Interaction with Csn2 in the Type II CRISPR-Cas System

Structure ◽  
2016 ◽  
Vol 24 (1) ◽  
pp. 70-79 ◽  
Author(s):  
Donghyun Ka ◽  
Hasup Lee ◽  
Yi-Deun Jung ◽  
Kyunggon Kim ◽  
Chaok Seok ◽  
...  
Keyword(s):  
Crystal Structure ◽  
Streptococcus Pyogenes ◽  
Type Ii

scholarly journals Structural and Biochemical Studies of Dihydrofolate Reductase from Streptococcus pyogenes as a Target for Antifolate Antibiotics

2018 ◽  
Author(s):  
Behnoush Hajian ◽  
Jolanta Krucinska ◽  
Michael Martins ◽  
Narendran G-Dayanan ◽  
Kishore Viswanathan ◽  
...  
Keyword(s):  
Crystal Structure ◽  
Streptococcus Pyogenes ◽  
Dihydrofolate Reductase ◽  
Wide Spectrum ◽  
Streptococcal Infections ◽  
Biochemical Studies ◽  
Live Bacteria ◽  
Dhfr Inhibitors ◽  
First Time ◽  
Structural Insights

ABSTRACTStreptococcus pyogenes, a beta-hemolytic bacterium, causes a wide spectrum of infections in human including pharyngitis, tonsillitis, scarlet fever, rheumatic fever, and necrotizing fasciitis. Streptococcal infections can also exist as co-infection with methicillin resistant Staphylococcus aureus (MRSA). Trimethoprim-sulfamethoxazole (TMP-SMX) combination has been used for treatment of S. pyogenes and MRSA co-infection. However, resistance to TMP, an inhibitor of dihydrofolate reductase enzyme (DHFR), has challenged the efficacy of TMP-SMX combination. We explored the activity of a series of novel DHFR inhibitors against S. pyogenes. This study identified potent inhibitors of DHFR enzyme from S. pyogenes with excellent inhibitory activity against the growth of the live bacteria. We determined, for the first time, the crystal structure of S. pyogenes DHFR which provides structural insights into design and development of antifolate agents against this global pathogen.

scholarly journals Crystal Structure of tRNA Adenosine Deaminase (TadA) fromAquifex aeolicus

2005 ◽  
Vol 280 (16) ◽  
pp. 16002-16008 ◽  
Author(s):  
Mitsuo Kuratani ◽  
Ryohei Ishii ◽  
Yoshitaka Bessho ◽  
Ryuya Fukunaga ◽  
Toru Sengoku ◽  
...  
Keyword(s):  
Crystal Structure ◽  
Adenosine Deaminase ◽  
Cytosine Deaminase ◽  
Amino Acid Residues ◽  
Aquifex Aeolicus ◽  
Stem Loop ◽  
Dimeric Structure ◽  
Docking Model ◽  
Tetrameric Structure ◽  
Yeast Cytosine Deaminase

The bacterial tRNA adenosine deaminase (TadA) generates inosine by deaminating the adenosine residue at the wobble position of tRNAArg-2. This modification is essential for the decoding system. In this study, we determined the crystal structure ofAquifex aeolicusTadA at a 1.8-Å resolution. This is the first structure of a deaminase acting on tRNA.A. aeolicusTadA has an α/β/α three-layered fold and forms a homodimer. TheA. aeolicusTadA dimeric structure is completely different from the tetrameric structure of yeast CDD1, which deaminates mRNA and cytidine, but is similar to the dimeric structure of yeast cytosine deaminase. However, in theA. aeolicusTadA structure, the shapes of the C-terminal helix and the regions between the β4 and β5 strands are quite distinct from those of yeast cytosine deaminase and a large cavity is produced. This cavity contains many conserved amino acid residues that are likely to be involved in either catalysis or tRNA binding. We made a docking model of TadA with the tRNA anticodon stem loop.

Crystal structure of Plasmodium falciparum adenosine deaminase reveals a novel binding pocket for inosine

2019 ◽  
Vol 667 ◽  
pp. 6-13
Author(s):  
Aritsara Jaruwat ◽  
Pinpunya Riangrungroj ◽  
Sakunrat Ubonprasert ◽  
Udom Sae-ueng ◽  
Buabarn Kuaprasert ◽  
...  
Keyword(s):  
Crystal Structure ◽  
Plasmodium Falciparum ◽  
Adenosine Deaminase ◽  
Binding Pocket

scholarly journals Crystal structure of Streptococcus pyogenes EndoS, an immunomodulatory endoglycosidase specific for human IgG antibodies

2014 ◽  
Vol 111 (18) ◽  
pp. 6714-6719 ◽  
Author(s):  
B. Trastoy ◽  
J. V. Lomino ◽  
B. G. Pierce ◽  
L. G. Carter ◽  
S. Gunther ◽  
...  
Keyword(s):  
Crystal Structure ◽  
Streptococcus Pyogenes ◽  
Igg Antibodies ◽  
Human Igg

Crystal structure of Staphylococcus aureus tRNA adenosine deaminase TadA in complex with RNA

2006 ◽  
Vol 13 (2) ◽  
pp. 153-159 ◽  
Author(s):  
Heather C Losey ◽  
Alexander J Ruthenburg ◽  
Gregory L Verdine
Keyword(s):  
Crystal Structure ◽  
Staphylococcus Aureus ◽  
Adenosine Deaminase

Crystal structure of ω transcriptional repressor encoded by Streptococcus pyogenes plasmid pSM19035 at 1.5 Å resolution 1 1Edited by R. Huber

2001 ◽  
Vol 314 (4) ◽  
pp. 789-796 ◽  
Author(s):  
Kazutaka Murayama ◽  
Peter Orth ◽  
Ana B de la Hoz ◽  
Juan C Alonso ◽  
Wolfram Saenger
Keyword(s):  
Crystal Structure ◽  
Streptococcus Pyogenes ◽  
Transcriptional Repressor

Model for Substrate Interactions in C5a Peptidase from Streptococcus pyogenes: A 1.9 Å Crystal Structure of the Active Form of ScpA

2009 ◽  
Vol 386 (3) ◽  
pp. 754-772 ◽  
Author(s):  
Todd F. Kagawa ◽  
Maurice R. O'Connell ◽  
Pania Mouat ◽  
Max Paoli ◽  
Paul W. O'Toole ◽  
...  
Keyword(s):  
Crystal Structure ◽  
Streptococcus Pyogenes ◽  
Active Form ◽  
Substrate Interactions
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