Gas Temperature Effect on Reactive Species Generation from the Atmospheric Pressure Air Plasma

2013 ◽  
Vol 10 (8) ◽  
pp. 686-697 ◽  
Author(s):  
Ho Young Kim ◽  
Sung Kil Kang ◽  
Hyoung Cheol Kwon ◽  
Hyun Woo Lee ◽  
Jae Koo Lee
Keyword(s):  
Temperature Effect ◽  
Atmospheric Pressure ◽  
Reactive Species ◽  
Gas Temperature ◽  
Air Plasma

Temporal gas temperature of atmospheric pressure air plasma

2021 ◽  
Author(s):  
Jiayin Li ◽  
Fan Wu ◽  
Yubin Xian ◽  
Xinpei Lu ◽  
Lanlan Nie
Keyword(s):  
Atmospheric Pressure ◽  
Gas Temperature ◽  
Air Plasma

scholarly journals Spore Viability and Cell Wall Integrity of Cordyceps pruinosa Treated with an Electric Shock-Free, Atmospheric-Pressure Air Plasma Jet

2019 ◽  
Vol 9 (18) ◽  
pp. 3921 ◽  
Author(s):  
Noh ◽  
Kim ◽  
Kim ◽  
Kim ◽  
Han ◽  
...  
Keyword(s):  
Cell Death ◽  
Cell Wall ◽  
Electric Shock ◽  
Atmospheric Pressure ◽  
Plasma Jet ◽  
Reactive Species ◽  
Cell Wall Integrity ◽  
Air Plasma ◽  
Spore Viability ◽  
Spore Cell

Atmospheric-pressure Ar plasma jets are known to be detrimental to Cordyceps pruinosa spores. However, it is not clear what kinds of reactive species are more effective with regard to fungal cell death. Herein, we study which reactive species plays pivotal roles in the death of fungal spores using an electric shock-free, atmospheric-pressure air plasma jet, simply called soft plasma jet. Plasma treatment significantly reduced the spore viability and damaged fungal DNA. As observed from the circular dichroism spectra, scanning electron microscope images, and flow cytometric measurements, cell wall integrity was decreased by reactive oxygen and nitrogen species (RONS) from the plasma itself and the plasma-activated water. Consequently, degradation of the spore cell wall allows RONS from the plasma to reach the intracellular components. Such plasma-induced intracellular RONS can attack spore DNA and other intracellular components, as confirmed by electrophoresis analysis and phosphorylated histone measurement. In addition, weakening of the spore cell wall allowed for the loss of intracellular components, which can lead to cell death. Plasma radicals were investigated by measuring the optical emission spectrum of the soft plasma jet, and intracellular reactive oxygen species were confirmed by measuring the fluorescence of 2′, 7′-dichlorodihydrofluorescein-diacetate (H2DCF-DA)-stained spores. The soft plasma jet generated considerable amounts of H2O2 and NOx but a very small number of OH radicals as compared to the atmospheric-pressure Arplasma jet; this indicates that plasma-induced long-lived reactive species (H2O2 and NOx) play an important role in the weakening of spore cell walls and cell death.

scholarly journals Cold Atmospheric Pressure Plasma Technology for Biomedical Application

2021 ◽  
Author(s):  
Rakesh Ruchel Khanikar ◽  
Heremba Bailung
Keyword(s):  
Atmospheric Pressure ◽  
Biomedical Applications ◽  
Biomedical Application ◽  
Biological Effects ◽  
Relative Concentration ◽  
Density Measurement ◽  
Reactive Species ◽  
Gas Temperature ◽  
Plasma Technology ◽  
Plasma Parameters

Cold plasma generated in an open environment with a temperature nearly around room temperature has recently been a topic of great importance. It has unlocked the door of plasma application in a new direction: biomedical applications. Cold atmospheric pressure (CAP) plasma comprises various neutral and charged reactive species, UV radiations, electric current/fields etc., which have several impactful effects on biological matter. Some of the significant biological effects of CAP plasma are inactivation of microorganism, stimulation of cell proliferation and tissue regeneration, destruction of cells by initializing apoptosis etc. Although the detailed mechanism of action of plasma on biomaterials is still not completely understood, some basic principles are known. Studies have indicated that the reactive oxygen species and nitrogen species (ROS, RNS) play a crucial role in the observed biological effects. In this perspective, this chapter first provides a brief discussion on the fundamentals of CAP plasma and its generation methods. Then a discussion on the optical diagnostics methods to characterize the plasma is provided. Optical emission spectroscopy (OES) is used to identify the reactive species and to measure their relative concentration. Other important plasma parameters such as gas temperature, electron/excitation temperature and electron density measurement methods using OES have also been discussed. Then a discussion on the application of CAP plasma in biomedical field is provided. A thorough understanding of biochemical reaction mechanisms involving highly reactive plasma species will further improve and extend CAP plasma technology in biomedical applications.

scholarly journals Anticancer Efficacy of Long-Term Stored Plasma-Activated Medium

2019 ◽  
Vol 9 (4) ◽  
pp. 801 ◽  
Author(s):  
Ngoc Nguyen ◽  
Hyung Park ◽  
Soon Hwang ◽  
Jong-Soo Lee ◽  
Sang Yang
Keyword(s):  
Atmospheric Pressure ◽  
Therapeutic Potential ◽  
Atmospheric Pressure Plasma ◽  
Reactive Species ◽  
The Body ◽  
Air Plasma ◽  
Anticancer Efficacy ◽  
Pressure Plasma ◽  
Anticancer Effects ◽  
Fresh Plasma

The therapeutic potential of nonthermal atmospheric-pressure plasma for cancer treatment via generation of reactive species, induction of decreased mitochondrial membrane potential, and sequential apoptosis has been reported in our previous studies. Nonthermal atmospheric-pressure plasma-activated medium produced by jetting air plasma above a liquid surface shows advantages over direct plasma such as storage and delivery to tissues inside the body. In this study, we demonstrated that plasma-activated medium can be stored for up to 6 months in a freezer and that the stored plasma-activated medium has anticancer effects similar to those of direct plasma. Plasma-activated medium stored for 6 months showed cytocidal effects on human cervical cancer HeLa cells that were comparable to the effects of fresh plasma-activated medium or direct plasma. Furthermore, the levels of reactive species in plasma-activated medium persisted for up to 6 months. These results indicate that therapeutic application of plasma-activated medium is applicable in plasma medicine and is a promising anticancer strategy.

Enhancement Propagation of Protocorms in Orchid (Cymbidium tracyanum L. Castle) by Cold Atmospheric Pressure Air Plasma Jet

2021 ◽  
Author(s):  
Sorapong Pavasupree ◽  
Nattapong Chanchula ◽  
Atipong Bootchanont ◽  
Chakkaphan Wattanawikkam ◽  
Pinit Jitjing ◽  
...  
Keyword(s):  
Atmospheric Pressure ◽  
Plasma Jet ◽  
Air Plasma
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