scholarly journals Anticancer Efficacy of Long-Term Stored Plasma-Activated Medium

2019 ◽  
Vol 9 (4) ◽  
pp. 801 ◽  
Author(s):  
Ngoc Nguyen ◽  
Hyung Park ◽  
Soon Hwang ◽  
Jong-Soo Lee ◽  
Sang Yang
Keyword(s):  
Atmospheric Pressure ◽  
Therapeutic Potential ◽  
Atmospheric Pressure Plasma ◽  
Reactive Species ◽  
The Body ◽  
Air Plasma ◽  
Anticancer Efficacy ◽  
Pressure Plasma ◽  
Anticancer Effects ◽  
Fresh Plasma

The therapeutic potential of nonthermal atmospheric-pressure plasma for cancer treatment via generation of reactive species, induction of decreased mitochondrial membrane potential, and sequential apoptosis has been reported in our previous studies. Nonthermal atmospheric-pressure plasma-activated medium produced by jetting air plasma above a liquid surface shows advantages over direct plasma such as storage and delivery to tissues inside the body. In this study, we demonstrated that plasma-activated medium can be stored for up to 6 months in a freezer and that the stored plasma-activated medium has anticancer effects similar to those of direct plasma. Plasma-activated medium stored for 6 months showed cytocidal effects on human cervical cancer HeLa cells that were comparable to the effects of fresh plasma-activated medium or direct plasma. Furthermore, the levels of reactive species in plasma-activated medium persisted for up to 6 months. These results indicate that therapeutic application of plasma-activated medium is applicable in plasma medicine and is a promising anticancer strategy.

scholarly journals Analysis of Plasma-activated Medium (PAM) in aqueous solution by an Atmospheric Pressure Plasma Jet (APPJ)

2018 ◽  
Vol 197 ◽  
pp. 02013 ◽  
Author(s):  
Andi Wibowo Kinandana ◽  
Sumariyah Sumariyah ◽  
Muhammad Nur
Keyword(s):  
Hydrogen Peroxide ◽  
Species Composition ◽  
Exposure Time ◽  
Atmospheric Pressure ◽  
Atmospheric Pressure Plasma ◽  
Plasma Jet ◽  
Reactive Species ◽  
Atmospheric Pressure Plasma Jet ◽  
Liquid Media ◽  
Pressure Plasma

Plasma-activated medium (PAM) has been produced by exposing a liquid media to Argon plasma jet. The jet plasma exposure to liquid media has produced reactive Oxygen species (ROS) in liquid phase. This study aims to determine the number of reactive species in plasma-activated medium. An atmospheric pressure plasma jet (APPJ) was generated with a dielectric barrier discharge (DBD) column by AC high voltage. Some parameters varied including exposure time; i.e. 5, 10, 15, 20, 25, and 30 min; and the distance between reactor and active media; i.e. 1, 2 and 3 cm. Some analysis conducted including variation of exposure times, the distances of reactor to PAM which affect produced concentration, and the reactive species composition in plasma-activated medium. In addition, temperature characteristics, pH levels, dissolved ozone and dissolved hydrogen peroxide concentrations were also observed in this study. The results showed that increased exposure time resulted in decreased pH, increased temperature and increased concentrations of ozone and hydrogen peroxide. The maximum reactive species composition was obtained at the distance between reactor and plasma-activated medium of 2 cm. Maximum reactive species composition obtained in this study has temperature of 29-30 Celsius degrees; pH 3.5; dissolved ozone 2.97 ppm; and Hydrogen Peroxide 215 ppm.

scholarly journals Cold Atmospheric Pressure Plasma in Wound Healing and Cancer Treatment

2020 ◽  
Vol 10 (19) ◽  
pp. 6898
Author(s):  
Lars Boeckmann ◽  
Mirijam Schäfer ◽  
Thoralf Bernhardt ◽  
Marie Luise Semmler ◽  
Ole Jung ◽  
...  
Keyword(s):  
Wound Healing ◽  
Cancer Therapy ◽  
Cancer Treatment ◽  
Atmospheric Pressure ◽  
Atmospheric Pressure Plasma ◽  
Reactive Species ◽  
Therapeutic Effects ◽  
Preclinical Research ◽  
Pressure Plasma ◽  
Cold Atmospheric Pressure Plasma

Plasma medicine is gaining increasing attention and is moving from basic research into clinical practice. While areas of application are diverse, much research has been conducted assessing the use of cold atmospheric pressure plasma (CAP) in wound healing and cancer treatment—two applications with entirely different goals. In wound healing, a tissue-stimulating effect is intended, whereas cancer therapy aims at killing malignant cells. In this review, we provide an overview of the latest clinical and some preclinical research on the efficacy of CAP in wound healing and cancer therapy. Furthermore, we discuss the current understanding of molecular signaling mechanisms triggered by CAP that grant CAP its antiseptic and tissue regenerating or anti-proliferative and cell death-inducing properties. For the efficacy of CAP in wound healing, already substantial evidence from clinical studies is available, while evidence for therapeutic effects of CAP in oncology is mainly from in vitro and in vivo animal studies. Efforts to elucidate the mode of action of CAP suggest that different components, such as ultraviolet (UV) radiation, electromagnetic fields, and reactive species, may act synergistically, with reactive species being regarded as the major effector by modulating complex and concentration-dependent redox signaling pathways.

scholarly journals Infrared Absorption Spectroscopic Study on Reaction between Self-Assembled Monolayers and Atmospheric-Pressure Plasma

2015 ◽  
Vol 2015 ◽  
pp. 1-7
Author(s):  
Masanori Shinohara ◽  
Katsuhiro Amano ◽  
Naoki Maruno ◽  
Yuta Yoshida ◽  
Yoshinobu Matsuda ◽  
...  
Keyword(s):  
Protein Interactions ◽  
Atmospheric Pressure ◽  
Water Clusters ◽  
Atmospheric Pressure Plasma ◽  
Air Plasma ◽  
Self Assembled Monolayer ◽  
Pressure Plasma ◽  
Assembled Monolayers ◽  
Self Assembled ◽  
Living Tissues

Plasma is becoming increasingly adopted in bioapplications such as plasma medicine and agriculture. This study investigates the interaction between plasma and molecules in living tissues, focusing on plasma-protein interactions. To this end, the reaction of air-pressure air plasma with NH2-terminated self-assembled monolayer is investigated by infrared spectroscopy in multiple internal reflection geometry. The atmospheric-pressure plasma decomposed the NH2components, the characteristic units of proteins. The decomposition is attributed to water clusters generated in the plasma, indicating that protein decomposition by plasma requires humid air.

scholarly journals Inactivation by helium cold atmospheric pressure plasma for Escherichia coli and Staphylococcus aureus

2019 ◽  
Vol 14 (1) ◽  
pp. 37-45 ◽  
Author(s):  
Khaled Lotfy ◽  
Sayed Mohammed Khalil ◽  
Hany Abd El-Raheem
Keyword(s):  
Flow Rate ◽  
Atmospheric Pressure ◽  
Plasma Temperature ◽  
Wavelength Region ◽  
Atmospheric Pressure Plasma ◽  
Optical Emission ◽  
Reactive Species ◽  
Pressure Plasma ◽  
Cold Atmospheric Pressure Plasma ◽  
The Impact

AbstractA helium cold atmospheric pressure plasma jet (HCAPPJ) driven by a commercial neon power supply was designed and utilized for inactivation bacteria. The generated reactive spices by HCAPPJ were investigated by optical emission spectroscopy. The reactive species of OH, OI, OI, N21+, N21+ and He were identified in the UV–Vis wavelength region. The reactive species was not detected between 200 nm and 300 nm, as the flow rate of helium gas increased that led to the plasma temperature reducing to a value near to the room temperature. In this work, we studied the impact of HCAPPJ on Gram-positive and Gram-negative bacteria. The survival amounts of the two types of bacteria were decreased vastly when the rate flow rate was equal to 10 L/min.

An experimental study of photoresist material etching by an atmospheric-pressure plasma jet with Ar/air mixed gas

2013 ◽  
Vol 79 (5) ◽  
pp. 683-689 ◽  
Author(s):  
LIJUN WANG ◽  
WENJUN NING ◽  
MINGZHENG FU ◽  
CHEN WU ◽  
SHENLI JIA
Keyword(s):  
Atmospheric Pressure ◽  
Atmospheric Pressure Plasma ◽  
Plasma Jet ◽  
Atmospheric Pressure Plasma Jet ◽  
Air Plasma ◽  
Pressure Plasma ◽  
Plasma Needle ◽  
Ar Plasma ◽  
Etched Surface ◽  
Plasma Pencil

AbstractIn this paper, electrical and optical emission spectrometer (OES) characteristics of an Ar/air atmospheric-pressure plasma jet (APPJ) based on the plasma needle and plasma pencil systems were investigated and analyzed. Electrical measurement results showed that the breakdown and working voltage of the jet increased with the increase of the ratio of air/Ar, and the emission intensity of Ar* significantly decreased. For the plasma needle, when the ratio of air/Ar reached 1, the OES characteristics of Ar/air were similar to those of air plasma, and the main excited species was N2*. For the plasma pencil, when a little air impurity was added in Ar, the emission intensities of N2* species will be significantly increased. Based on these two APPJ systems, photoresist materials were etched, etched results showed that the etched surface was easier to be oxidized with the addition of air into Ar. The etched surface was cleaner with pure Ar plasma with scanning substrate methods than that with the Ar/air mixture. Etched results of higher ratios of air/Ar plasma were similar to those of air plasma.

scholarly journals Applications of Cold Atmospheric Pressure Plasma in Dentistry

2021 ◽  
Vol 11 (5) ◽  
pp. 1975
Author(s):  
Aline C. Borges ◽  
Konstantin G. Kostov ◽  
Rodrigo S. Pessoa ◽  
Geraldo M.A. de Abreu ◽  
Gabriela de M.G. Lima ◽  
...  
Keyword(s):  
Atmospheric Pressure ◽  
Biological Effects ◽  
Atmospheric Pressure Plasma ◽  
Reactive Species ◽  
Electrically Conducting ◽  
Pressure Plasma ◽  
Cold Plasmas ◽  
Cold Atmospheric Pressure Plasma ◽  
Ultraviolet Photons ◽  
Oral Oncology

Plasma is an electrically conducting medium that responds to electric and magnetic fields. It consists of large quantities of highly reactive species, such as ions, energetic electrons, exited atoms and molecules, ultraviolet photons, and metastable and active radicals. Non-thermal or cold plasmas are partially ionized gases whose electron temperatures usually exceed several tens of thousand degrees K, while the ions and neutrals have much lower temperatures. Due to the presence of reactive species at low temperature, the biological effects of non-thermal plasmas have been studied for application in the medical area with promising results. This review outlines the application of cold atmospheric pressure plasma (CAPP) in dentistry for the control of several pathogenic microorganisms, induction of anti-inflammatory, tissue repair effects and apoptosis of cancer cells, with low toxicity to healthy cells. Therefore, CAPP has potential to be applied in many areas of dentistry such as cariology, periodontology, endodontics and oral oncology.

Cold-Air Atmospheric Pressure Plasma Against Clostridium difficile Spores: A Potential Alternative for the Decontamination of Hospital Inanimate Surfaces

2015 ◽  
Vol 36 (6) ◽  
pp. 742-744 ◽  
Author(s):  
Tânia Claro ◽  
Orla J. Cahill ◽  
Niall O’Connor ◽  
Stephen Daniels ◽  
Hilary Humphreys
Keyword(s):  
Clostridium Difficile ◽  
Atmospheric Pressure ◽  
Atmospheric Pressure Plasma ◽  
Infect Control Hosp ◽  
Air Plasma ◽  
Cold Air ◽  
Pressure Plasma ◽  
Environmental Surfaces ◽  
Potential Alternative ◽  
Single Jet

AbstractClostridium difficile spores survive for months on environmental surfaces and are highly resistant to decontamination. We evaluated the effect of cold-air plasma against C. difficile spores. The single-jet had no effect while the multi-jet achieved 2–3 log10 reductions in spore counts and may augment traditional decontamination.Infect Control Hosp Epidemiol 2015;00(0):1–3

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