Proteomic analysis of nuclear proteins from proliferative and differentiated human colonic intestinal epithelial cells

PROTEOMICS ◽  
2003 ◽  
Vol 4 (1) ◽  
pp. 93-105 ◽  
Author(s):  
Natacha Turck ◽  
Sophie Richert ◽  
Patrick Gendry ◽  
Jeanne Stutzmann ◽  
Michèle Kedinger ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Proteomic Analysis ◽  
Intestinal Epithelial Cells ◽  
Nuclear Proteins ◽  
Intestinal Epithelial

Differential proteomic analysis of HT29 Cl.16E and intestinal epithelial cells by LC ESI/QTOF mass spectrometry

2009 ◽  
Vol 72 (5) ◽  
pp. 865-873 ◽  
Author(s):  
Paolo Nanni ◽  
Laura Mezzanotte ◽  
Giulia Roda ◽  
Alessandra Caponi ◽  
Fredrik Levander ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Epithelial Cells ◽  
Proteomic Analysis ◽  
Intestinal Epithelial Cells ◽  
Intestinal Epithelial

Laminin isoforms: biological roles and effects on the intracellular distribution of nuclear proteins in intestinal epithelial cells

2005 ◽  
Vol 303 (2) ◽  
pp. 494-503 ◽  
Author(s):  
Natacha Turck ◽  
Isabelle Gross ◽  
Patrick Gendry ◽  
Jeanne Stutzmann ◽  
Jean-Noël Freund ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Intestinal Epithelial Cells ◽  
Intracellular Distribution ◽  
Nuclear Proteins ◽  
Intestinal Epithelial

scholarly journals Poly(adenosine diphosphate ribose) synthetase activity in nuclei of dividing and of non-dividing but differentiating intestinal epithelial cells

1979 ◽  
Vol 180 (3) ◽  
pp. 455-461 ◽  
Author(s):  
J W Porteous ◽  
H M Furneaux ◽  
C K Pearson ◽  
C M Lake ◽  
A Morrison
Keyword(s):  
Small Intestine ◽  
Epithelial Cells ◽  
Guinea Pig ◽  
Intestinal Epithelial Cells ◽  
Adenosine Diphosphate ◽  
Crypt Cell ◽  
Nuclear Proteins ◽  
Synthetase Activity ◽  
Intestinal Epithelial ◽  
Cell Nuclei

Poly(ADP-ribose) synthetase activity is found in nuclei of regenerating epithelial cells in the lower half of the crypts of guinea-pig small intestine. Nuclei from non-dividing but differentiating and maturing cells in the upper crypts and on the villi contain no more than about 10% of the synthetase activity of lower-crypt cell nuclei. The product in the active nuclei is shown to be 80% poly(ADP-ribosylated) protein and 20% mono(ADP-ribosylated) protein; 60% of thetotal labelled product was attached to acid-soluble proteins (including histones), and 40% to acid-insoluble (non-histone) proteins. The average number of ADP-ribosyl units in the oligomeric chains of the poly(ADP-ribosylated) proteins was 15 but the range of sizes of (ADP-ribose) oligomers attached to nuclear proteins was smaller in villus than in crypt cell nuclei.

Three-dimensional organization and functional relationships of endocytotic compartments in pig intestinal epithelial cells

1992 ◽  
Vol 50 (1) ◽  
pp. 576-577
Author(s):  
Julian P. Heath ◽  
Buford L. Nichols ◽  
László G. Kömüves
Keyword(s):  
Electron Microscopy ◽  
Epithelial Cells ◽  
Intestinal Epithelial Cells ◽  
Three Dimensional ◽  
Intestinal Epithelial ◽  
Cell Surfaces ◽  
Basal Surface ◽  
Functional Relationships

The newborn pig intestine is adapted for the rapid and efficient absorption of nutrients from colostrum. In enterocytes, colostral proteins are taken up into an apical endocytotic complex of channels that transports them to target organelles or to the basal surface for release into the circulation. The apical endocytotic complex of tubules and vesicles clearly is a major intersection in the routes taken by vesicles trafficking to and from the Golgi, lysosomes, and the apical and basolateral cell surfaces.Jejunal tissues were taken from piglets suckled for up to 6 hours and prepared for electron microscopy and immunocytochemistry as previously described.

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