Proteomic analysis of Trichinella spiralis proteins in intestinal epithelial cells after culture with their larvae by shotgun LC–MS/MS approach

2012 ◽  
Vol 75 (8) ◽  
pp. 2375-2383 ◽  
Author(s):  
Zhong Quan Wang ◽  
Lei Wang ◽  
Jing Cui
Keyword(s):  
Epithelial Cells ◽  
Proteomic Analysis ◽  
Intestinal Epithelial Cells ◽  
Trichinella Spiralis ◽  
Intestinal Epithelial

Differential proteomic analysis of HT29 Cl.16E and intestinal epithelial cells by LC ESI/QTOF mass spectrometry

2009 ◽  
Vol 72 (5) ◽  
pp. 865-873 ◽  
Author(s):  
Paolo Nanni ◽  
Laura Mezzanotte ◽  
Giulia Roda ◽  
Alessandra Caponi ◽  
Fredrik Levander ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Epithelial Cells ◽  
Proteomic Analysis ◽  
Intestinal Epithelial Cells ◽  
Intestinal Epithelial

scholarly journals RNAi-mediated silencing of Trichinella spiralis serpin-type serine protease inhibitors results in a reduction in larval infectivity

2020 ◽  
Vol 51 (1) ◽  
Author(s):  
Nana Yi ◽  
Pengcheng Yu ◽  
Lijia Wu ◽  
Zhaokun Liu ◽  
Jingzhe Guan ◽  
...  
Keyword(s):  
Survival Rate ◽  
Epithelial Cells ◽  
Serine Protease ◽  
Protease Inhibitors ◽  
Intestinal Epithelial Cells ◽  
Trichinella Spiralis ◽  
Intestinal Epithelial ◽  
Serine Protease Inhibitors

AbstractTrichinella spiralis serpin-type serine protease inhibitors (TsSPIs) are expressed in adult worms (AW), newborn larvae (NBL) and muscle larvae (ML) of T. spiralis, with the ML stage demonstrating the highest expression level. This study aims to determine TsSPI functions in larval viability and invasion of intestinal epithelial cells in vitro, as well as their development, survival, and fecundity in vivo via RNAi. TsSPI-specific siRNAs and dsRNA were transfected into ML by incubation. The silencing effect of TsSPI transcription and expression was determined using qPCR and western blot, respectively. After incubation in 60 ng/μL dsRNA–TsSPI for 3 days, larval TsSPI mRNA and protein expression levels were reduced by 68.7% and 68.4% (P < 0.05), respectively. dsRNA-mediated silencing of TsSPI significantly impacted larval invasion into intestinal epithelial cells in vitro but did not affect the survival rate of larvae. After challenge with dsRNA–TsSPI-treated ML, mice exhibited a 56.0% reduction in intestinal AW burden and 56.9% reduction in ML burden (P < 0.05), but NBL production of female AW remained the same (P > 0.05). Our results revealed that RNAi-mediated silencing of TsSPI expression in T. spiralis significantly reduced larval infectivity and survival in the host but had no effect on the survival rate and fecundity. Furthermore, TsSPIs have no effect on the growth and reproduction of parasites but may be directly involved in regulating the interaction of T. spiralis and the host. Therefore, TsSPIs are crucial in the process of T. spiralis larval invasion and parasite survival in the host.

Proteomic analysis of nuclear proteins from proliferative and differentiated human colonic intestinal epithelial cells

PROTEOMICS ◽  
2003 ◽  
Vol 4 (1) ◽  
pp. 93-105 ◽  
Author(s):  
Natacha Turck ◽  
Sophie Richert ◽  
Patrick Gendry ◽  
Jeanne Stutzmann ◽  
Michèle Kedinger ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Proteomic Analysis ◽  
Intestinal Epithelial Cells ◽  
Nuclear Proteins ◽  
Intestinal Epithelial
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