Incorporation of fetal DNA detection assay in a noninvasive RhD diagnostic test

2010 ◽  
Vol 30 (10) ◽  
pp. 1010-1012 ◽  
Author(s):  
Yuchi Li ◽  
Jeffrey A. Kazzaz ◽  
Leonard H. Kellner ◽  
Stephen A. Brown
Keyword(s):  
Diagnostic Test ◽  
Dna Detection ◽  
Fetal Dna ◽  
Detection Assay

scholarly journals Development of a Multiplexed Assay for Detection ofLeishmania donovaniandLeishmania infantumProtein Biomarkers in Urine Samples of Patients with Visceral Leishmaniasis

2019 ◽  
Vol 57 (5) ◽  
Author(s):  
Claudia Abeijon ◽  
Fabiana Alves ◽  
Severine Monnerat ◽  
Monique Wasunna ◽  
Jane Mbui ◽  
...  
Keyword(s):  
Visceral Leishmaniasis ◽  
Diagnostic Test ◽  
Leishmania Donovani ◽  
Protein Biomarkers ◽  
Serological Tests ◽  
Assay Sensitivity ◽  
Content Type ◽  
Detection Assay ◽  
Previously Treated ◽  
New Biomarkers

ABSTRACTVisceral leishmaniasis (VL) is a serious and fatal disease caused by the parasitesLeishmania infantumandLeishmania donovani. The gold standard diagnostic test for VL is the demonstration of parasites or their DNA in spleen, lymph node, or bone marrow aspirates. Serological tests exist but cannot distinguish active VL from either prior exposure to the parasites or previously treated VL disease. Using mass spectroscopy, we have previously identified threeL. infantumprotein biomarkers (Li-isd1,Li-txn1, andLi-ntf2) in the urine of VL patients and developed a sensitive and specific urine-based antigen detection assay for the diagnosis of VL that occurs in Brazil (where VL is caused byL. infantum). However, unpublished observations from our laboratory at DetectoGen showed that these biomarkers were detected in only 55% to 60% of VL patients from India and Kenya, where the disease is caused byL. donovani. Here, we report the discovery and characterization of two new biomarkers ofL. donovani(Ld-mao1andLd-ppi1) present in the urine of VL patients from these two countries. Capture enzyme-linked immunosorbent assays using specific rabbit IgG and chicken IgY were developed, and the assays had sensitivities of 44.4% and 28.8% for the detection ofLd-mao1andLd-ppi1, respectively. In contrast, a multiplexed assay designed to simultaneously detect all five leishmanial biomarkers markedly increased the assay sensitivity to 82.2%. These results validate the utility of leishmanial protein biomarkers found in the urine of VL patients as powerful tools for the development of an accurate diagnostic test for this disease.

Clinical results after the implementation of cell-free fetal DNA detection in maternal plasma

2018 ◽  
Vol 44 (8) ◽  
pp. 1369-1376 ◽  
Author(s):  
Cristina Martínez-Payo ◽  
Isabel Bada-Bosch ◽  
María Martínez-Moya ◽  
Tirso Pérez-Medina
Keyword(s):  
Dna Detection ◽  
Clinical Results ◽  
Maternal Plasma ◽  
Fetal Dna ◽  
Cell Free Fetal Dna

Fetal DNA detection in maternal plasma throughout gestation

2005 ◽  
Vol 117 (2-3) ◽  
pp. 243-248 ◽  
Author(s):  
Silvia Galbiati ◽  
Maddalena Smid ◽  
Dania Gambini ◽  
Augusto Ferrari ◽  
Gabriella Restagno ◽  
...  
Keyword(s):  
Dna Detection ◽  
Maternal Plasma ◽  
Fetal Dna

Sensitive and selective viral DNA detection assay via microbead-based rolling circle amplification

2008 ◽  
Vol 18 (22) ◽  
pp. 5871-5874 ◽  
Author(s):  
Eric Schopf ◽  
Nicholas O. Fischer ◽  
Yong Chen ◽  
Jeffrey B.-H. Tok
Keyword(s):  
Dna Detection ◽  
Rolling Circle Amplification ◽  
Viral Dna ◽  
Rolling Circle ◽  
Detection Assay

Double positive effect of adding hexaethyelene glycol when optimizing the hybridization efficiency of a microring DNA detection assay

2017 ◽  
Vol 405 ◽  
pp. 321-328 ◽  
Author(s):  
Anabelle Van Eeghem ◽  
Sam Werquin ◽  
Jan-Willem Hoste ◽  
Arne Goes ◽  
Els Vanderleyden ◽  
...  
Keyword(s):  
Dna Detection ◽  
Double Positive ◽  
Detection Assay ◽  
Hybridization Efficiency ◽  
Positive Effect

scholarly journals SNP-Based Fetal DNA Detection in Maternal Serum Using the HID-Ion AmpliSeq™ Identity Panel

2017 ◽  
Vol 41 (2) ◽  
pp. 41
Author(s):  
Sohee Cho ◽  
Ji Hyun Lee ◽  
Chong Jai Kim ◽  
Moon Young Kim ◽  
Kun Woo Kim ◽  
...  
Keyword(s):  
Dna Detection ◽  
Maternal Serum ◽  
Fetal Dna
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