Identification of the reaction products of rosmarinic acid synthase from cell cultures ofColeus blumei by ion spray mass spectrometry and tandem mass spectrometry

1993 ◽  
Vol 4 (3) ◽  
pp. 131-134 ◽  
Author(s):  
Maike Petersen ◽  
Jörg W. Metzger
Keyword(s):  
Mass Spectrometry ◽  
Tandem Mass Spectrometry ◽  
Cell Cultures ◽  
Rosmarinic Acid ◽  
Tandem Mass ◽  
Reaction Products ◽  
Ion Spray Mass Spectrometry ◽  
Rosmarinic Acid Synthase

scholarly journals Determination of 4-Methylimidazole in Ammonia Caramel Using Gas Chromatography–Tandem Mass Spectrometry (GC-MS/MS)

2018 ◽  
Vol 2018 ◽  
pp. 1-5
Author(s):  
Martyna N. Wieczorek ◽  
Krzysztof Przygoński ◽  
Henryk H. Jeleń
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Tandem Mass Spectrometry ◽  
Mass Spectrometry Analysis ◽  
Maillard Reaction Products ◽  
Tandem Mass ◽  
Reaction Products ◽  
Isobutyl Chloroformate ◽  
Chromatography Tandem Mass Spectrometry ◽  
Production Of Ammonia

One of Maillard reaction products formed in the production of ammonia caramel is 4(5)-methylimidazole (4-MeI) classified as carcinogen. A method of 4-MeI determination based on ion-pair extraction and derivatisation with isobutyl chloroformate with subsequent gas chromatography-tandem mass spectrometry analysis was proposed. Tandem mass spectrometry was applied to reduce the influence of matrix and increase the selectivity and sensitivity of the method. Triple quadrupole GC-MS system was used for this study. The collision energies were optimized for MRM mode. The detection (LOD) and quantification limits (LOQ) of the elaborated method were 17 and 37.8 μg kg−1, respectively, repeatability was <15% RSD for analyzed caramel samples, and the recovery for 4-MeI was 101%. Comparison of MS/MS with SIM detection on the same instrument proved almost 30 times lower LODs achieved by tandem mass spectrometry compared to SIM. Described method can be routinely used for monitoring 4-MeI as a quality and safety marker in the production of ammonia caramel.

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