Mechanism of action of (2-haloethyl)nitrosoureas on DNA: discrimination between alternative pathways of DNA base modification by 1,3-bis(2-fluoroethyl)-1-nitrosourea by using specific deuterium labeling and identification of reaction products by HPLC/tandem mass spectrometry

1990 ◽  
Vol 112 (8) ◽  
pp. 3178-3187 ◽  
Author(s):  
Ali Naghipur ◽  
Michael G. Ikonomou ◽  
Paul Kebarle ◽  
J. William Lown
Keyword(s):  
Mass Spectrometry ◽  
Tandem Mass Spectrometry ◽  
Mechanism Of Action ◽  
Tandem Mass ◽  
Reaction Products ◽  
Deuterium Labeling ◽  
Alternative Pathways ◽  
Base Modification ◽  
Dna Base

High-throughput mass spectrometry: the mechanism of sudan azo dye fragmentation by ESI tandem mass spectrometry and extensive deuterium labeling experiments

2007 ◽  
Vol 42 (8) ◽  
pp. 1057-1061 ◽  
Author(s):  
Leonardo Di Donna ◽  
Antonio De Nino ◽  
Loredana Maiuolo ◽  
Fabio Mazzotti ◽  
Anna Napoli ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Tandem Mass Spectrometry ◽  
High Throughput ◽  
Azo Dye ◽  
Tandem Mass ◽  
Deuterium Labeling

scholarly journals Determination of 4-Methylimidazole in Ammonia Caramel Using Gas Chromatography–Tandem Mass Spectrometry (GC-MS/MS)

2018 ◽  
Vol 2018 ◽  
pp. 1-5
Author(s):  
Martyna N. Wieczorek ◽  
Krzysztof Przygoński ◽  
Henryk H. Jeleń
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Tandem Mass Spectrometry ◽  
Mass Spectrometry Analysis ◽  
Maillard Reaction Products ◽  
Tandem Mass ◽  
Reaction Products ◽  
Isobutyl Chloroformate ◽  
Chromatography Tandem Mass Spectrometry ◽  
Production Of Ammonia

One of Maillard reaction products formed in the production of ammonia caramel is 4(5)-methylimidazole (4-MeI) classified as carcinogen. A method of 4-MeI determination based on ion-pair extraction and derivatisation with isobutyl chloroformate with subsequent gas chromatography-tandem mass spectrometry analysis was proposed. Tandem mass spectrometry was applied to reduce the influence of matrix and increase the selectivity and sensitivity of the method. Triple quadrupole GC-MS system was used for this study. The collision energies were optimized for MRM mode. The detection (LOD) and quantification limits (LOQ) of the elaborated method were 17 and 37.8 μg kg−1, respectively, repeatability was <15% RSD for analyzed caramel samples, and the recovery for 4-MeI was 101%. Comparison of MS/MS with SIM detection on the same instrument proved almost 30 times lower LODs achieved by tandem mass spectrometry compared to SIM. Described method can be routinely used for monitoring 4-MeI as a quality and safety marker in the production of ammonia caramel.

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