One-step Preparative Separation of Two Polyhydroxystilbenes from Rheum likiangense Sam. by High-speed Counter-current Chromatography

2012 ◽  
Vol 23 (6) ◽  
pp. 684-688 ◽  
Author(s):  
Xiaoyan Wang ◽  
Honglun Wang ◽  
Chenxu Ding ◽  
Yourui Suo
Keyword(s):  
High Speed ◽  
Preparative Separation ◽  
One Step ◽  
Counter Current

One-step Separation and Purification of Four Phenolic Acids from Stenoloma chusanum (L.) Ching by Medium-pressure Liquid Chromatography and High-speed Counter-current Chromatography

2019 ◽  
Vol 9 (2) ◽  
pp. 138-143
Author(s):  
Tianyun Li ◽  
Xiling Dai ◽  
Yichen Li ◽  
Guozheng Huang ◽  
Jianguo Cao
Keyword(s):  
Natural Products ◽  
Liquid Chromatography ◽  
Phenolic Acids ◽  
High Speed ◽  
High Efficiency ◽  
Total Phenolic ◽  
Medium Pressure ◽  
Medium Pressure Liquid Chromatography ◽  
One Step ◽  
Counter Current

Background:Stenoloma chusanum (L.) Ching is a Chinese traditional medicinal fern with high total flavonoid and total phenolic content. Traditionally, phenolic compounds were separated by using column chromatography, which is relatively inefficient. </P><P> Objective: This study aims to use an efficient method to separate natural products from S. chusanum by Medium-Pressure Liquid Chromatography (MPLC) and High-Speed Counter-Current Chromatography (HSCCC).Methods:In the present research, firstly, a sample (2.5 g) from the dichloromethane extract of S. chusanum was separated by MPLC. Next, fraction P5 was purified by HSCCC with a two-phase solvent system composed of hexane-ethyl acetate-methanol-water (HEMWat) at a volume ratio of 2:4:1:4 (v/v/v/v). </P><P> Result: Four phenolic acids were obtained and their structures were identified by means of NMR and ESI-mass analysis. They were identified as: 1) protocatechuic acid (34 mg, purity 90.1%), 2) syringic acid (66 mg, purity 99.0%), 3) p-hydroxybenzoic acid (5 mg, purity 91.2%) and 4) vanillic acid (6 mg, purity 99.3%).Conclusion:The combination of MPLC and HSCCC is a high-efficiency separation method for natural products. This is the first report with regard to the separation of four phenolic acids in one step by MPLC and HSCCC from S. chusanum (L.) Ching.

One-step Separation of Three Flavonoids from Poacynum hendersonii by High-speed Counter-current Chromatography

2011 ◽  
Vol 22 (5) ◽  
pp. 450-454 ◽  
Author(s):  
Junyou Shi ◽  
Guoliang Li ◽  
Honglun Wang ◽  
Jie Zheng ◽  
Yourui Suo ◽  
...  
Keyword(s):  
High Speed ◽  
One Step ◽  
Counter Current

scholarly journals Preparative separation of two subsidiary colors of FD&C Yellow No. 5 (Tartrazine) using spiral high-speed counter-current chromatography

2014 ◽  
Vol 1343 ◽  
pp. 91-100 ◽  
Author(s):  
Adrian Weisz ◽  
Clark D. Ridge ◽  
Jose A. Roque ◽  
Eugene P. Mazzola ◽  
Yoichiro Ito
Keyword(s):  
High Speed ◽  
Preparative Separation ◽  
Counter Current

scholarly journals One-Step Separation of Antioxidant Compounds from Erythrina variegata by High Speed Counter-Current Chromatography

2014 ◽  
Vol 53 (5) ◽  
pp. 730-735 ◽  
Author(s):  
Qi Liu ◽  
Jingang Yu ◽  
Xiaoyun Liao ◽  
Peisen Zhang ◽  
Xiaoqing Chen
Keyword(s):  
High Speed ◽  
Antioxidant Compounds ◽  
Erythrina Variegata ◽  
One Step ◽  
Counter Current

Preparative separation of polyphenols from the flowers of Paeonia lactiflora Pall. by high-speed counter-current chromatography

2014 ◽  
Vol 947-948 ◽  
pp. 62-67 ◽  
Author(s):  
Xikai Shu ◽  
Wenjuan Duan ◽  
Feng Liu ◽  
Xingang Shi ◽  
Yanling Geng ◽  
...  
Keyword(s):  
High Speed ◽  
Preparative Separation ◽  
Paeonia Lactiflora ◽  
Counter Current

scholarly journals Preparative Separation of Diterpene Lactones and Flavones from Andrographis paniculate Using Off-Line Two-Dimensional High-Speed Counter-Current Chromatography

Molecules ◽  
2019 ◽  
Vol 24 (3) ◽  
pp. 620 ◽  
Author(s):  
Xiaowei Sun ◽  
Huijiao Yan ◽  
Yujie Zhang ◽  
Xiao Wang ◽  
Dawei Qin ◽  
...  
Keyword(s):  
Ethyl Acetate ◽  
Petroleum Ether ◽  
High Speed ◽  
Spectroscopic Methods ◽  
Two Dimensional ◽  
Preparative Separation ◽  
Counter Current ◽  
First Time

Seven diterpene lactones, andrographolide (1), isoandrographolide (2), neo-andrographolide (3), 14-deoxy-11,12-didehydroandrographolide (4), 14-deoxyandrographiside (5), 14-deoxy-11,12-didehydroandrographiside (6), 3,14-dideoxyandrographolide (10), and three flavones, andrographidine C (7), andrographidine A (8), 5-hydroxy-7,8-dimethoxyflavanone (9) have been successfully and efficiently isolated from A. paniculata using an off-line two dimensional (2D) high-speed counter-current chromatography (HSCCC) method for the first time. For the first dimension HSCCC separation, petroleum ether-ethyl acetate-methanol-water 3:7:5:5 (v/v) was employed to isolate 14.4 mg of compound 1, 3.1 mg of compound 2, 7.8 mg of compound 3, and 18.0 mg of compound 4 from 200 mg of the A. paniculata extract. For the second dimension HSCCC separation, petroleum ether-ethyl acetate-methanol-water 2:8:1:9 (v/v) and 5:5:6:4 (v/v) were employed to isolate the collected fractions ranged from 55 to 79 min and the flow out fraction, respectively, which led to 5.1 mg of compound 5, 4.4 mg of compound 6, 2.4 mg of compound 7, 3.3 mg of compound 8, 4.0 mg of compound 9, 7.0 mg of compound 10. The structures of these diterpene lactones and flavones were elucidated by extensive spectroscopic methods.

scholarly journals Development of online-storage inner-recycling counter-current chromatography for the preparative separation of complex components of alkylphenols from sarcotesta of Ginkgo biloba L.

RSC Advances ◽  
2018 ◽  
Vol 8 (60) ◽  
pp. 34321-34330 ◽  
Author(s):  
Daijie Wang ◽  
Xiangyun Song ◽  
Huijiao Yan ◽  
Mengmeng Guo ◽  
Ruiming Fu ◽  
...  
Keyword(s):  
Natural Products ◽  
Ginkgo Biloba ◽  
High Speed ◽  
Separation Method ◽  
Preparative Separation ◽  
Online Storage ◽  
Counter Current ◽  
Complex Components

High-speed counter-current chromatography (HSCCC) is becoming an effective and non-absorptive separation method from natural products.

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