The relation between the fine structure of smooth-muscle cells and elastogenesis in the chick-embryo aorta

1971 ◽  
Vol 104 (4) ◽  
pp. 253-260 ◽  
Author(s):  
Anna Kadar ◽  
D. L. Gardner ◽  
Valerie Bush
Keyword(s):  
Smooth Muscle ◽  
Fine Structure ◽  
Chick Embryo ◽  
Smooth Muscle Cells ◽  
Muscle Cells

scholarly journals FINE STRUCTURE OF SMOOTH MUSCLE CELLS GROWN IN TISSUE CULTURE

1971 ◽  
Vol 49 (1) ◽  
pp. 21-34 ◽  
Author(s):  
Gordon R. Campbell ◽  
Yasuo Uehara ◽  
Gerda Mark ◽  
Geoffrey Burnstock
Keyword(s):  
Electron Microscopy ◽  
Smooth Muscle ◽  
Fine Structure ◽  
Cell Membrane ◽  
Smooth Muscle Cells ◽  
Phase Contrast ◽  
Muscle Cells ◽  
Different Types ◽  
Membrane Infoldings

The fine structure of smooth muscle cells of the embryo chicken gizzard cultured in monolayer was studied by phase-contrast optics and electron microscopy. The smooth muscle cells were irregular in shape, but tended to be elongate. The nucleus usually contained prominent nucleoli and was large in relation to the cell body. When fixed with glutaraldehyde, three different types of filaments were noted in the cytoplasm: thick (150–250 A in diameter) and thin (30–80 A in diameter) myofilaments, many of which were arranged in small bundles throughout the cytoplasm and which were usually associated with dark bodies; and filaments with a diameter of 80–110 A which were randomly orientated and are not regarded as myofilaments. Some of the aggregated ribosomes were helically arranged. Mitochondria, Golgi apparatus, and dilated rough endoplasmic reticulum were prominent. In contrast to in vivo muscle cells, micropinocytotic vesicles along the cell membrane were rare and dense areas were usually confined to cell membrane infoldings. These cells are compared to in vivo embryonic smooth muscle and adult muscle after treatment with estrogen. Monolayers of cultured smooth muscle will be of particular value in relating ultrastructural features to functional observations on the same cells.

Effect of Estrogen and Progesterone on Oviduct Smooth Muscle: An Ultrastructural and Cytochemical Study

1971 ◽  
Vol 29 ◽  
pp. 552-553
Author(s):  
R. K. Nayak ◽  
A. S. H. Wu
Keyword(s):  
Smooth Muscle ◽  
Fine Structure ◽  
Smooth Muscle Cells ◽  
Adenosine Triphosphatase ◽  
Muscle Cells ◽  
Hormone Treatment ◽  
Estradiol Benzoate ◽  
Cellular Level ◽  
Cytochemical Study ◽  
Subcellular Level

The smooth muscle cells of the Fallopian tube, although similar in structure to those of other organs are unique in their response to the action of steroid hormones. To our knowledge few studies have as yet been published about the fine structure of oviduct smooth muscle cell. This fact has induced us to study the influence of estrogen and progesterone upon the ultrastructure of oviduct smooth muscle cells in an attempt to elucidate the action of hormones on the cellular level. Attempts were also made to demonstrate the presence of adenosine triphosphatase (ATP-ase) in these cells at subcellular level.In Experiment I, prepubertal rabbits (8 weeks old) were given 7 daily intramuscular injections of estradiol benzoate (5 μg/day) and progesterone (2 mg/day) in propylene glycol. In Experiment II, rabbits were ovariectomized at 8 weeks of age and received hormone treatment same as that given in Experiment I, starting 40 days after surgery.

Immunogold localization of HMB-45 antigen in pulmonary lymphangiomyomatosis

1995 ◽  
Vol 53 ◽  
pp. 998-999
Author(s):  
J.M. Minda ◽  
E. Dessy ◽  
G. G. Pietra
Keyword(s):  
Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Room Temperature ◽  
Osmium Tetroxide ◽  
Muscle Cells ◽  
Ultrastructural Localization ◽  
Reproductive Age ◽  
Thin Sections ◽  
Smooth Muscle Proliferation ◽  
Hmb 45

Pulmonary lymphangiomyomatosis (PLAM) is a rare disease occurring exclusively in women of reproductive age. It involves the lungs, lymph nodes and lymphatic ducts. In the lungs, it is characterized by the proliferation of smooth muscle cells around lymphatics in the bronchovascular bundles, lobular septa and pleura The nature of smooth muscle proliferation in PLAM is still unclear. Recently, reactivity of the smooth muscle cells for HMB-45, a melanoma-related antigen has been reported by immunohistochemistry. The purpose of this study was the ultrastructural localization of HMB-45 immunoreactivity in these cells using gold-labeled antibodies.Lung tissue from three cases of PLAM, referred to our Institution for lung transplantation, was embedded in either Poly/Bed 812 post-fixed in 1% osmium tetroxide, or in LR White, without osmication. For the immunogold technique, thin sections were placed on Nickel grids and incubated with affinity purified, monoclonal anti-melanoma antibody HMB-45 (1:1) (Enzo Diag. Co) overnight at 4°C. After extensive washing with PBS, grids were treated with Goat-anti-mouse-IgG-Gold (5nm) (1:10) (Amersham Life Sci) for 1 hour, at room temperature.

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