scholarly journals Fine Structure of the Sinusoidal Wall in the Liver of Fresh-Water Catfish (Parasilurus asotus), with Special Reference to the Smooth Muscle Cells

1983 ◽  
Vol 46 (1) ◽  
pp. 125-130 ◽  
Author(s):  
Hiroshi SATO ◽  
Torao YAMAMOTO
Keyword(s):  
Smooth Muscle ◽  
Fine Structure ◽  
Smooth Muscle Cells ◽  
Special Reference ◽  
Fresh Water ◽  
Muscle Cells ◽  
Parasilurus Asotus ◽  
Sinusoidal Wall

scholarly journals FINE STRUCTURE OF SMOOTH MUSCLE CELLS GROWN IN TISSUE CULTURE

1971 ◽  
Vol 49 (1) ◽  
pp. 21-34 ◽  
Author(s):  
Gordon R. Campbell ◽  
Yasuo Uehara ◽  
Gerda Mark ◽  
Geoffrey Burnstock
Keyword(s):  
Electron Microscopy ◽  
Smooth Muscle ◽  
Fine Structure ◽  
Cell Membrane ◽  
Smooth Muscle Cells ◽  
Phase Contrast ◽  
Muscle Cells ◽  
Different Types ◽  
Membrane Infoldings

The fine structure of smooth muscle cells of the embryo chicken gizzard cultured in monolayer was studied by phase-contrast optics and electron microscopy. The smooth muscle cells were irregular in shape, but tended to be elongate. The nucleus usually contained prominent nucleoli and was large in relation to the cell body. When fixed with glutaraldehyde, three different types of filaments were noted in the cytoplasm: thick (150–250 A in diameter) and thin (30–80 A in diameter) myofilaments, many of which were arranged in small bundles throughout the cytoplasm and which were usually associated with dark bodies; and filaments with a diameter of 80–110 A which were randomly orientated and are not regarded as myofilaments. Some of the aggregated ribosomes were helically arranged. Mitochondria, Golgi apparatus, and dilated rough endoplasmic reticulum were prominent. In contrast to in vivo muscle cells, micropinocytotic vesicles along the cell membrane were rare and dense areas were usually confined to cell membrane infoldings. These cells are compared to in vivo embryonic smooth muscle and adult muscle after treatment with estrogen. Monolayers of cultured smooth muscle will be of particular value in relating ultrastructural features to functional observations on the same cells.

Physiological Features of Visceral Smooth Muscle Cells, With Special Reference to Receptors and Ion Channels

1998 ◽  
Vol 78 (3) ◽  
pp. 811-920 ◽  
Author(s):  
H. KURIYAMA ◽  
K. KITAMURA ◽  
T. ITOH ◽  
R. INOUE
Keyword(s):  
Smooth Muscle ◽  
Ion Channels ◽  
Patch Clamp ◽  
Smooth Muscle Cells ◽  
Special Reference ◽  
Muscle Cells ◽  
Relaxation Cycle ◽  
Visceral Smooth Muscle ◽  
Work Done ◽  
The Way

Kuriyama, H., K. Kitamura, T. Itoh, and R. Inoue. Physiological Features of Visceral Smooth Muscle Cells, With Special Reference to Receptors and Ion Channels. Physiol. Rev. 78: 811–920, 1998. — Visceral smooth muscle cells (VSMC) play an essential role, through changes in their contraction-relaxation cycle, in the maintenance of homeostasis in biological systems. The features of these cells differ markedly by tissue and by species; moreover, there are often regional differences within a given tissue. The biophysical features used to investigate ion channels in VSMC have progressed from the original extracellular recording methods (large electrode, single or double sucrose gap methods), to the intracellular (microelectrode) recording method, and then to methods for recording from membrane fractions (patch-clamp, including cell-attached patch-clamp, methods). Remarkable advances are now being made thanks to the application of these more modern biophysical procedures and to the development of techniques in molecular biology. Even so, we still have much to learn about the physiological features of these channels and about their contribution to the activity of both cell and tissue. In this review, we take a detailed look at ion channels in VSMC and at receptor-operated ion channels in particular; we look at their interaction with the contraction-relaxation cycle in individual VSMC and especially at the way in which their activity is related to Ca2+ movements and Ca2+ homeostasis in the cell. In sections ii and iii, we discuss research findings mainly derived from the use of the microelectrode, although we also introduce work done using the patch-clamp procedure. These sections cover work on the electrical activity of VSMC membranes (sect. ii) and on neuromuscular transmission (sect. iii). In sections iv and v, we discuss work done, using the patch-clamp procedure, on individual ion channels (Na+, Ca2+, K+, and Cl−; sect. iv) and on various types of receptor-operated ion channels (with or without coupled GTP-binding proteins and voltage dependent and independent; sect. v). In sect. vi, we look at work done on the role of Ca2+ in VSMC using the patch-clamp procedure, biochemical procedures, measurements of Ca2+ transients, and Ca2+ sensitivity of contractile proteins of VSMC. We discuss the way in which Ca2+ mobilization occurs after membrane activation (Ca2+ influx and efflux through the surface membrane, Ca2+ release from and uptake into the sarcoplasmic reticulum, and dynamic changes in Ca2+ within the cytosol). In this article, we make only limited reference to vascular smooth muscle research, since we reviewed the features of ion channels in vascular tissues only recently.

Effect of Estrogen and Progesterone on Oviduct Smooth Muscle: An Ultrastructural and Cytochemical Study

1971 ◽  
Vol 29 ◽  
pp. 552-553
Author(s):  
R. K. Nayak ◽  
A. S. H. Wu
Keyword(s):  
Smooth Muscle ◽  
Fine Structure ◽  
Smooth Muscle Cells ◽  
Adenosine Triphosphatase ◽  
Muscle Cells ◽  
Hormone Treatment ◽  
Estradiol Benzoate ◽  
Cellular Level ◽  
Cytochemical Study ◽  
Subcellular Level

The smooth muscle cells of the Fallopian tube, although similar in structure to those of other organs are unique in their response to the action of steroid hormones. To our knowledge few studies have as yet been published about the fine structure of oviduct smooth muscle cell. This fact has induced us to study the influence of estrogen and progesterone upon the ultrastructure of oviduct smooth muscle cells in an attempt to elucidate the action of hormones on the cellular level. Attempts were also made to demonstrate the presence of adenosine triphosphatase (ATP-ase) in these cells at subcellular level.In Experiment I, prepubertal rabbits (8 weeks old) were given 7 daily intramuscular injections of estradiol benzoate (5 μg/day) and progesterone (2 mg/day) in propylene glycol. In Experiment II, rabbits were ovariectomized at 8 weeks of age and received hormone treatment same as that given in Experiment I, starting 40 days after surgery.

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