scholarly journals Intracellular redox state revealed by in vivo 31 P MRS measurement of NAD+ and NADH contents in brains

2013 ◽  
Vol 71 (6) ◽  
pp. 1959-1972 ◽  
Author(s):  
Ming Lu ◽  
Xiao-Hong Zhu ◽  
Yi Zhang ◽  
Wei Chen
Keyword(s):  
Redox State ◽  
Intracellular Redox

scholarly journals In vivo assessment of intracellular redox state in rat liver using hyperpolarized [1‐ 13 C]Alanine

2017 ◽  
Vol 77 (5) ◽  
pp. 1741-1748 ◽  
Author(s):  
Jae Mo Park ◽  
Chalermchai Khemtong ◽  
Shie‐Chau Liu ◽  
Ralph E. Hurd ◽  
Daniel M. Spielman
Keyword(s):  
Rat Liver ◽  
Redox State ◽  
In Vivo Assessment ◽  
Intracellular Redox

In‐Vivo Two‐Photon Visualization and Quantitative Detection of Redox State of Cancer

2022 ◽  
Author(s):  
Wei Wang ◽  
Chenlu Wang ◽  
Guoming Liu ◽  
Long Jin ◽  
Zexi Lin ◽  
...  
Keyword(s):  
Redox State ◽  
Quantitative Detection ◽  
Two Photon

In vivo recording of epidermal stem cell redox state

2017 ◽  
Vol 108 ◽  
pp. S38
Author(s):  
Alexander Martin Wolf ◽  
Shigeo Ohta
Keyword(s):  
Stem Cell ◽  
Redox State ◽  
Epidermal Stem Cell

scholarly journals Resolving diurnal dynamics of the chloroplastic glutathione redox state in Arabidopsis reveals its photosynthetically-derived oxidation

2021 ◽  
Author(s):  
Zechariah Haber ◽  
Nardy Lampl ◽  
Andreas J Meyer ◽  
Einat Zelinger ◽  
Matanel Hipsch ◽  
...  
Keyword(s):  
Redox State ◽  
Fluorescent Protein ◽  
Operating Efficiency ◽  
High Temporal Resolution ◽  
Fluctuating Light ◽  
Gsh Metabolism ◽  
Glutathione Cycle ◽  
Green Fluorescent ◽  
Glutathione Redox

Abstract Plants are subjected to fluctuations in light intensity, and this causes unbalanced photosynthetic electron fluxes and overproduction of reactive oxygen species (ROS). Electrons needed for ROS detoxification are drawn, at least partially, from the cellular glutathione (GSH) pool via the ascorbate-glutathione cycle. Here, we explore the dynamics of the chloroplastic glutathione redox potential (chl-EGSH) using high-temporal-resolution monitoring of Arabidopsis (Arabidopsis thaliana) lines expressing the reduction-oxidation sensitive green fluorescent protein 2 (roGFP2in chloroplasts. This was carried out over several days, under dynamic environmental conditions and in correlation with PSII operating efficiency. Peaks in chl-EGSH oxidation during dark-to-light and light-to-dark transitions were observed. Increasing light intensities triggered a binary oxidation response, with a threshold around the light saturating point, suggesting two regulated oxidative states of the chl-EGSH. These patterns were not affected in npq1 plants, which are impaired in nonphotochemical quenching. Oscillations between the two oxidation states were observed under fluctuating light in WT and npq1 plants, but not in pgr5 plants, suggesting a role for PSI photoinhibition in regulating the chl-EGSH dynamics. Remarkably, pgr5 plants showed an increase in chl-EGSH oxidation during the nights following light stresses, linking daytime photoinhibition and nighttime GSH metabolism. This work provides a systematic view of the dynamics of the in vivo chloroplastic glutathione redox state during varying light conditions.

Intracellular Redox State and Stimulation of Gluconeogenesis by Glucagon and Norepinephrine in the Perfused Rat Liver

1980 ◽  
Vol 87 (1) ◽  
pp. 153-166 ◽  
Author(s):  
Tsukasa SUGANO ◽  
Masakazu SHIOTA ◽  
Takashi TANAKA ◽  
Yhoichi MIYAMAE ◽  
Masakazu SHIMADA ◽  
...  
Keyword(s):  
Rat Liver ◽  
Redox State ◽  
Perfused Rat Liver ◽  
Stimulation Of ◽  
Intracellular Redox

Small Stress Proteins: Modulation of Intracellular Redox State and Protection Against Oxidative Stress

2002 ◽  
pp. 171-184 ◽  
Author(s):  
André-Patrick Arrigo ◽  
Catherine Paul ◽  
Cécile Ducasse ◽  
Olivier Sauvageot ◽  
Carole Kretz-Remy
Keyword(s):  
Oxidative Stress ◽  
Redox State ◽  
Stress Proteins ◽  
Intracellular Redox

scholarly journals The Lack of Mitochondrial Thioredoxin TRXo1 Affects In Vivo Alternative Oxidase Activity and Carbon Metabolism under Different Light Conditions

2019 ◽  
Vol 60 (11) ◽  
pp. 2369-2381 ◽  
Author(s):  
Igor Florez-Sarasa ◽  
Toshihiro Obata ◽  
N�stor Fern�ndez Del-Saz ◽  
Jean-Philippe Reichheld ◽  
Etienne H Meyer ◽  
...  
Keyword(s):  
Carbon Metabolism ◽  
Redox State ◽  
Redox Regulation ◽  
Alternative Oxidase ◽  
Tricarboxylic Acid Cycle ◽  
Reduced Form ◽  
Photosynthetic Parameters ◽  
Primary Metabolism ◽  
Redox Systems

Abstract The alternative oxidase (AOX) constitutes a nonphosphorylating pathway of electron transport in the mitochondrial respiratory chain that provides flexibility to energy and carbon primary metabolism. Its activity is regulated in vitro by the mitochondrial thioredoxin (TRX) system which reduces conserved cysteines residues of AOX. However, in vivo evidence for redox regulation of the AOX activity is still scarce. In the present study, the redox state, protein levels and in vivo activity of the AOX in parallel to photosynthetic parameters were determined in Arabidopsis knockout mutants lacking mitochondrial trxo1 under moderate (ML) and high light (HL) conditions, known to induce in vivo AOX activity. In addition, 13C- and 14C-labeling experiments together with metabolite profiling were performed to better understand the metabolic coordination between energy and carbon metabolism in the trxo1 mutants. Our results show that the in vivo AOX activity is higher in the trxo1 mutants at ML while the AOX redox state is apparently unaltered. These results suggest that mitochondrial thiol redox systems are responsible for maintaining AOX in its reduced form rather than regulating its activity in vivo. Moreover, the negative regulation of the tricarboxylic acid cycle by the TRX system is coordinated with the increased input of electrons into the AOX pathway. Under HL conditions, while AOX and photosynthesis displayed similar patterns in the mutants, photorespiration is restricted at the level of glycine decarboxylation most likely as a consequence of redox imbalance.

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