scholarly journals Intracellular redox-responsive nanocarrier for plasmid delivery: in vitro characterization and in vivo studies in mice

2016 ◽  
Vol Volume 11 ◽  
pp. 5245-5256 ◽  
Author(s):  
Lifen Zhang ◽  
Yushun Zhang ◽  
Zhenzhen Chen ◽  
Yuling He
Keyword(s):  
In Vivo Studies ◽  
In Vitro Characterization ◽  
Redox Responsive ◽  
Plasmid Delivery ◽  
Intracellular Redox

Crosslinked nanocarriers based upon poly(ethylene imine) for systemic plasmid delivery: In vitro characterization and in vivo studies in mice

2007 ◽  
Vol 118 (3) ◽  
pp. 370-380 ◽  
Author(s):  
Michael Neu ◽  
Oliver Germershaus ◽  
Shirui Mao ◽  
Karl-Heinz Voigt ◽  
Martin Behe ◽  
...  
Keyword(s):  
In Vivo Studies ◽  
Ethylene Imine ◽  
In Vitro Characterization ◽  
Poly Ethylene ◽  
Plasmid Delivery

scholarly journals Triaza-based amphiphilic chelators: Synthetic route, in vitro characterization and in vivo studies of their Ga(III) and Al(III) chelates

2010 ◽  
Vol 104 (10) ◽  
pp. 1051-1062 ◽  
Author(s):  
Arsénio de Sá ◽  
M. Isabel M. Prata ◽  
Carlos F.G.C. Geraldes ◽  
João P. André
Keyword(s):  
In Vivo Studies ◽  
Synthetic Route ◽  
In Vitro Characterization

scholarly journals Oxidized Alginate Dopamine Conjugate: In Vitro Characterization for Nose-to-Brain Delivery Application

Materials ◽  
2021 ◽  
Vol 14 (13) ◽  
pp. 3495
Author(s):  
Adriana Trapani ◽  
Filomena Corbo ◽  
Gennaro Agrimi ◽  
Nicoletta Ditaranto ◽  
Nicola Cioffi ◽  
...  
Keyword(s):  
Ex Vivo ◽  
Near Field ◽  
Neuroblastoma Cell ◽  
Fluorescein Isothiocyanate ◽  
Neuroblastoma Cell Line ◽  
In Vivo Studies ◽  
Brain Delivery ◽  
In Vitro Characterization

Background: The blood–brain barrier (BBB) bypass of dopamine (DA) is still a challenge for supplying it to the neurons of Substantia Nigra mainly affected by Parkinson disease. DA prodrugs have been studied to cross the BBB, overcoming the limitations of DA hydrophilicity. Therefore, the aim of this work is the synthesis and preliminary characterization of an oxidized alginate-dopamine (AlgOX-DA) conjugate conceived for DA nose-to-brain delivery. Methods: A Schiff base was designed to connect oxidized polymeric backbone to DA and both AlgOX and AlgOX-DA were characterized in terms of Raman, XPS, FT-IR, and 1H- NMR spectroscopies, as well as in vitro mucoadhesive and release tests. Results: Data demonstrated that AlgOX-DA was the most mucoadhesive material among the tested ones and it released the neurotransmitter in simulated nasal fluid and in low amounts in phosphate buffer saline. Results also demonstrated the capability of scanning near-field optical microscopy to study the structural and fluorescence properties of AlgOX, fluorescently labeled with fluorescein isothiocyanate microstructures. Interestingly, in SH-SY5Y neuroblastoma cell line up to 100 μg/mL, no toxic effect was derived from AlgOX and AlgOX-DA in 24 h. Conclusions: Overall, the in vitro performances of AlgOX and AlgOX-DA conjugates seem to encourage further ex vivo and in vivo studies in view of nose-to-brain administration.

Inhalable liposomal dry powder of gemcitabine-HCl: Formulation, in vitro characterization and in vivo studies

2015 ◽  
Vol 496 (2) ◽  
pp. 886-895 ◽  
Author(s):  
Manit Gandhi ◽  
Tosha Pandya ◽  
Ravi Gandhi ◽  
Sagar Patel ◽  
Rajashree Mashru ◽  
...  
Keyword(s):  
In Vivo Studies ◽  
Dry Powder ◽  
In Vitro Characterization

scholarly journals Glibenclamide Nanocrystal-Loaded Bioactive Polymeric Scaffolds for Skin Regeneration: In Vitro Characterization and Preclinical Evaluation

Pharmaceutics ◽  
2021 ◽  
Vol 13 (9) ◽  
pp. 1469
Author(s):  
Julie R. Youssef ◽  
Nabila A. Boraie ◽  
Heba F. Ibrahim ◽  
Fatma A. Ismail ◽  
Riham M. El-Moslemany
Keyword(s):  
Wound Healing ◽  
Skin Regeneration ◽  
In Vivo Studies ◽  
Dermal Substitute ◽  
Full Thickness ◽  
In Vitro Characterization ◽  
Polymeric Scaffolds ◽  
Chitosan Composite

Skin restoration following full-thickness injury poses significant clinical challenges including inflammation and scarring. Medicated scaffolds formulated from natural bioactive polymers present an attractive platform for promoting wound healing. Glibenclamide was formulated in collagen/chitosan composite scaffolds to fulfill this aim. Glibenclamide was forged into nanocrystals with optimized colloidal properties (particle size of 352.2 nm, and polydispersity index of 0.29) using Kolliphor as a stabilizer to allow loading into the hydrophilic polymeric matrix. Scaffolds were prepared by the freeze drying method using different total polymer contents (3–6%) and collagen/chitosan ratios (0.25–2). A total polymer content of 3% at a collagen/chitosan ratio of 2:1 (SCGL3-2) was selected based on the results of in vitro characterization including the swelling index (1095.21), porosity (94.08%), mechanical strength, rate of degradation and in vitro drug release. SCGL3-2 was shown to be hemocompatible based on the results of protein binding, blood clotting and percentage hemolysis assays. In vitro cell culture studies on HSF cells demonstrated the biocompatibility of nanocrystals and SCGL3-2. In vivo studies on a rat model of a full-thickness wound presented rapid closure with enhanced histological and immunohistochemical parameters, revealing the success of the scaffold in reducing inflammation and promoting wound healing without scar formation. Hence, SCGL3-2 could be considered a potential dermal substitute for skin regeneration.

In vitro characterization, and in vivo studies of crosslinked lactosaminated carboxymethyl chitosan nanoparticles

2011 ◽  
Vol 84 (3) ◽  
pp. 1048-1053 ◽  
Author(s):  
Hua Zheng ◽  
Xueqiong Zhang ◽  
Yihua Yin ◽  
Fuliang Xiong ◽  
Xiaoyu Gong ◽  
...  
Keyword(s):  
Chitosan Nanoparticles ◽  
Carboxymethyl Chitosan ◽  
In Vivo Studies ◽  
In Vitro Characterization
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