scholarly journals In vivo assessment of intracellular redox state in rat liver using hyperpolarized [1‐ 13 C]Alanine

2017 ◽  
Vol 77 (5) ◽  
pp. 1741-1748 ◽  
Author(s):  
Jae Mo Park ◽  
Chalermchai Khemtong ◽  
Shie‐Chau Liu ◽  
Ralph E. Hurd ◽  
Daniel M. Spielman
Keyword(s):  
Rat Liver ◽  
Redox State ◽  
In Vivo Assessment ◽  
Intracellular Redox

Intracellular Redox State and Stimulation of Gluconeogenesis by Glucagon and Norepinephrine in the Perfused Rat Liver

1980 ◽  
Vol 87 (1) ◽  
pp. 153-166 ◽  
Author(s):  
Tsukasa SUGANO ◽  
Masakazu SHIOTA ◽  
Takashi TANAKA ◽  
Yhoichi MIYAMAE ◽  
Masakazu SHIMADA ◽  
...  
Keyword(s):  
Rat Liver ◽  
Redox State ◽  
Perfused Rat Liver ◽  
Stimulation Of ◽  
Intracellular Redox

scholarly journals The development of gluconeogenesis in rat liver. Effects of glucagon and ether

1970 ◽  
Vol 120 (2) ◽  
pp. 385-392 ◽  
Author(s):  
Helen Philippidis ◽  
F. J. Ballard
Keyword(s):  
Rat Liver ◽  
Redox State ◽  
Phosphoenolpyruvate Carboxykinase ◽  
Fold Increase ◽  
Similar Increase ◽  
Ether Anaesthesia ◽  
Foetal Liver ◽  
Foetal Rat ◽  
Newborn Animals

1. Administration of glucagon to foetal rats produced a 10–15-fold increase in hepatic phosphoenolpyruvate carboxykinase activity together with a similar increase in the overall pathway of pyruvate conversion into glycogen in liver slices. 2. Glucagon was without effect on gluconeogenesis in vivo, which remained at approx. 0.1% of the incorporation as measured in newborn animals. 3. The apparent discrepancy between these results was due to the ether anaesthesia that was required for experimentation in vivo. Under conditions when minimal ether was used, the rates of labelling of glycogen from [3-14C]pyruvate in vivo were increased 10–20-fold and there was an additional stimulus by glucagon. 4. Ether anaesthesia produced a more reduced redox state of the foetal liver cytosol and lowered the ATP/ADP concentration ratio. 5. It is proposed that these effects are significant in the limitation of gluconeogenesis in the foetal rat liver, so that only with high phosphoenolpyruvate carboxykinase activity, high ATP concentration and a relatively oxidized cytosol redox state will a functional gluconeogenic pathway be present.

scholarly journals Control of the redox state of the nicotinamide–adenine dinucleotide couple in rat liver cytoplasm

1972 ◽  
Vol 126 (1) ◽  
pp. 59-65 ◽  
Author(s):  
Marion Stubbs ◽  
R. L. Veech ◽  
H. A. Krebs
Keyword(s):  
Rat Liver ◽  
Redox State ◽  
Adenine Nucleotide ◽  
Adenine Nucleotides ◽  
Phosphoglycerate Kinase ◽  
Phosphorylation State ◽  
Crotyl Alcohol ◽  
Order Of Magnitude ◽  
The Individual

1. A study has been made of the ability of rat liver in vivo to maintain equilibrium in the combined glyceraldehyde 3-phosphate dehydrogenase, 3-phosphoglycerate kinase and lactate dehydrogenase reactions, i.e. in the system: [Formula: see text] Attempts were made to upset equilibrium. The [lactate]/[pyruvate] ratio was rapidly changed by injection of ethanol or crotyl alcohol, and the value of [ATP]/[ADP][HPO42-] was rapidly changed by injection of ethionine or carbonyl cyanide p-trifluoromethoxy-phenylhydrazone. 2. The concentrations of the metabolites occurring in the above equation were measured in freeze-clamped liver. 3. Although the injected agents caused large changes in the concentrations of the individual components, near-equilibrium in the system was maintained, as indicated by the fact that the value of [ATP]/[ADP][HPO42-], referred to as the phosphorylation state of the adenine nucleotides, measured directly agreed with the value calculated for equilibrium conditions from the above equation. 4. The results are discussed and taken to confirm that the order of magnitude of the value of the redox state of the cytoplasmic NAD couple in rat liver is controlled by the phosphorylation state of the adenine nucleotide system.

scholarly journals Intracellular redox state revealed by in vivo 31 P MRS measurement of NAD+ and NADH contents in brains

2013 ◽  
Vol 71 (6) ◽  
pp. 1959-1972 ◽  
Author(s):  
Ming Lu ◽  
Xiao-Hong Zhu ◽  
Yi Zhang ◽  
Wei Chen
Keyword(s):  
Redox State ◽  
Intracellular Redox

The Metabolite Ratio as a Function of Chloral Hydrate Dose and Intracellular Redox State in the Perfused Rat Liver

1987 ◽  
Vol 60 (5) ◽  
pp. 325-329 ◽  
Author(s):  
Toshihiro Kawamoto ◽  
Tatsuya Hobara ◽  
Haruo Kobayashi ◽  
Susumu Iwamoto ◽  
Tsunemi Sakai ◽  
...  
Keyword(s):  
Rat Liver ◽  
Redox State ◽  
Chloral Hydrate ◽  
Perfused Rat Liver ◽  
Metabolite Ratio ◽  
Intracellular Redox

scholarly journals Unacylated ghrelin does not alter mitochondrial function, redox state and triglyceride content in rat liver in vivo

2015 ◽  
Vol 4 ◽  
pp. 1-7 ◽  
Author(s):  
Gianluca Gortan Cappellari ◽  
Michela Zanetti ◽  
Annamaria Semolic ◽  
Pierandrea Vinci ◽  
Giulia Ruozi ◽  
...  
Keyword(s):  
Rat Liver ◽  
Mitochondrial Function ◽  
Redox State ◽  
Unacylated Ghrelin ◽  
Triglyceride Content

scholarly journals The development of gluconeogenesis in rat liver. Experiments in vivo

1969 ◽  
Vol 113 (4) ◽  
pp. 651-657 ◽  
Author(s):  
Helen Philippidis ◽  
F. J. Ballard
Keyword(s):  
Rat Liver ◽  
Redox State ◽  
Fold Increase ◽  
Newborn Rats ◽  
Foetal Liver ◽  
Tracer Experiments ◽  
Newborn Animals

1. The injection of substrate amounts of lactate into newborn rats produced an increase in the concentration of phosphoenolpyruvate in liver. Similar experiments with foetal rats showed no increase in phosphoenolpyruvate concentration although pyruvate formation was observed. 2. The administration of pyruvate to foetal rats was also without effect on the hepatic phosphoenolpyruvate concentration, although a 20-fold increase in this was observed when pyruvate was injected into newborn animals. 3. Analogous experiments with aspartate produced qualitatively similar differences between foetal and newborn rats. 4. When [14C]-lactate, -pyruvate or -aspartate was injected into foetal or newborn rats incorporation of radioactivity into liver glucose was observed only in the newborn animals. 5. Lactate/pyruvate ratios of 213 in foetal liver and 13·5 in the livers of newborn rats indicated a relatively reduced environment in the cytosol of foetal liver. This difference in redox state was illustrated experimentally by a greater conversion of pyruvate into lactate and an increased formation of malate in foetal liver. 6. Although both the substrate-loading and tracer experiments indicated a block in gluconeogenesis in foetal liver at the stage of conversion of oxaloacetate into phosphoenolpyruvate, gluconeogenesis was also hindered by a highly reduced environment.

Sign in / Sign up

Export Citation Format

Share Document