Evidence for mouse sulfhydryl oxidase‐assisted cross‐linking of major seminal vesicle proteins

2019 ◽  
Vol 86 (11) ◽  
pp. 1682-1693
Author(s):  
Rubhadevi Balu ◽  
Shiyam Sundar Ramachandran ◽  
Sudhakar Gandhi Paramasivam
Keyword(s):  
Seminal Vesicle ◽  
Cross Linking ◽  
Sulfhydryl Oxidase ◽  
Vesicle Proteins

scholarly journals Purification and identification of transglutaminase from mouse coagulating gland and its cross-linking activity among seminal vesicle secretion proteins

2008 ◽  
Vol 876 (2) ◽  
pp. 198-202 ◽  
Author(s):  
Huan-Chin Tseng ◽  
Han-Jia Lin ◽  
P.S. Sudhakar Gandhi ◽  
Chia-Yih Wang ◽  
Yee-Hsiung Chen
Keyword(s):  
Seminal Vesicle ◽  
Cross Linking ◽  
Vesicle Secretion ◽  
Coagulating Gland

scholarly journals Heterogenous Turnover of Sperm and Seminal Vesicle Proteins in the Mouse Revealed by Dynamic Metabolic Labeling

2012 ◽  
Vol 11 (6) ◽  
pp. M111.014993 ◽  
Author(s):  
Amy J. Claydon ◽  
Steven A. Ramm ◽  
Andrea Pennington ◽  
Jane L. Hurst ◽  
Paula Stockley ◽  
...  
Keyword(s):  
Seminal Vesicle ◽  
Metabolic Labeling ◽  
Vesicle Proteins

Effect of chronic stress on expression and secretion of seminal vesicle proteins in adult rats

Andrologia ◽  
2020 ◽  
Author(s):  
Sitthichai Iamsaard ◽  
Saranya Tongpan ◽  
Supataechasit Yannasithinon ◽  
Supatcharee Arun ◽  
Alexander T. H. Wu ◽  
...  
Keyword(s):  
Seminal Vesicle ◽  
Chronic Stress ◽  
Adult Rats ◽  
Vesicle Proteins

scholarly journals Deletion of a Seminal Gene Cluster Reinforces a Crucial Role of SVS2 in Male Fertility

2019 ◽  
Vol 20 (18) ◽  
pp. 4557 ◽  
Author(s):  
Miyuki Shindo ◽  
Masafumi Inui ◽  
Woojin Kang ◽  
Moe Tamano ◽  
Cai Tingwei ◽  
...  
Keyword(s):  
Seminal Vesicle ◽  
Gene Cluster ◽  
Loxp Site ◽  
Male Mice ◽  
Internal Fertilization ◽  
Immune Attack ◽  
Sperm Membrane ◽  
Encoding Gene ◽  
And Function ◽  
Vesicle Proteins

Multiple genes, whose functions or expression are overlapping, compensate for the loss of one gene. A gene cluster in the mouse genome encodes five seminal vesicle proteins (SVS2, SVS3, SVS4, SVS5, and SVS6). These proteins are produced by male rodents and function in formation of the copulatory plug following mating. SVS2 plays an essential role in the successful internal fertilization by protecting the sperm membrane against a uterine immune attack. We hypothesized that the four remaining seminal vesicle proteins (SVPs) of this gene cluster may partially/completely compensate for the deficiency of SVS2. For confirming our hypothesis, we generated mice lacking the entire SVP-encoding gene cluster and compared their fecundity with Svs2-deficient (Svs2−/−) mice; that is, mice deficient in Svs2 alone. A single loxP site remained after the deletion of the Svs2 gene. Therefore, we inserted another loxP site by combining the CRISPR/Cas9 system with single-stranded oligodeoxynucleotides (ssODN). Male mice lacking the entire SVP-encoding gene cluster (Svs2–6−/− mice) and thereby all five SVP proteins, generated by the deletion of 100kbp genomic DNA, showed low fecundity. However, the fecundity level was comparable with that from Svs2−/− male mice. Our results demonstrate that SVS3, SVS4, SVS5, and SVS6 do not function in the protection of sperm against a uterine immune attack in the absence of SVS2. Thus, Svs2 is the critical gene in the SVP gene cluster.

scholarly journals Rat Seminal Vesicle FAD-dependent Sulfhydryl Oxidase

2001 ◽  
Vol 276 (17) ◽  
pp. 13830-13837 ◽  
Author(s):  
Béatrice Benayoun ◽  
Annick Esnard-Fève ◽  
Sandrine Castella ◽  
Yves Courty ◽  
Frédéric Esnard
Keyword(s):  
Seminal Vesicle ◽  
Sulfhydryl Oxidase ◽  
Rat Seminal Vesicle

Svp-3, a third polymorphic locus for mouse seminal vesicle proteins

1983 ◽  
Vol 21 (7-8) ◽  
pp. 797-800 ◽  
Author(s):  
R. Moutier ◽  
M. F. Bertrand
Keyword(s):  
Seminal Vesicle ◽  
Polymorphic Locus ◽  
Vesicle Proteins
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