Effect of chronic stress on expression and secretion of seminal vesicle proteins in adult rats

Andrologia ◽  
2020 ◽  
Author(s):  
Sitthichai Iamsaard ◽  
Saranya Tongpan ◽  
Supataechasit Yannasithinon ◽  
Supatcharee Arun ◽  
Alexander T. H. Wu ◽  
...  
Keyword(s):  
Seminal Vesicle ◽  
Chronic Stress ◽  
Adult Rats ◽  
Vesicle Proteins

Induction of functional cytodifferentiation in the epithelium of tissue recombinants. I. Homotypic seminal vesicle recombinants

Development ◽  
1989 ◽  
Vol 106 (2) ◽  
pp. 219-234 ◽  
Author(s):  
S.J. Higgins ◽  
P. Young ◽  
J.R. Brody ◽  
G.R. Cunha
Keyword(s):  
Seminal Vesicle ◽  
Time Course ◽  
Full Range ◽  
Seminal Vesicles ◽  
Neonatal Rat ◽  
Secretory Proteins ◽  
Functional Variation ◽  
Adult Rats ◽  
Normal Functional

Functional cytodifferentiation of seminal vesicle epithelium was investigated in tissue recombinants. Neonatal rat and mouse seminal vesicles were separated into epithelium and mesenchyme using trypsin. Epithelium and mesenchyme were then recombined in vitro to form interspecific rat/mouse homotypic recombinants. Growth as renal grafts in adult male athymic mice resulted in seminal vesicle morphogenesis in 70% of the recombinants (the remaining 30% failed to grow). Functional cytodifferentiation was judged by the expression of the major androgen-dependent secretory proteins characteristic of the seminal vesicles of adult rats and mice. Antibodies specific for each of these proteins were used to screen tissue sections by immunocytochemistry and to probe protein extracts by immunoblotting techniques. The heterospecific recombinants synthesized the full range of seminal vesicle secretory proteins that typifies the species providing the epithelium of the recombinant, not the mesenchyme. There was little functional variation between individual recombinants. The time course of development corresponded to that of intact neonatal seminal vesicles grown under the same conditions. Morphogenesis and functional cytodifferentiation were not evident after one week, but were well advanced after two weeks. Seminal vesicle recombinants grown for three weeks were indistinguishable morphologically and functionally from normal adult seminal vesicles. In addition, the ability of adult seminal vesicle epithelium to be induced to proliferate was examined. In association with neonatal seminal vesicle mesenchyme, the epithelium of the adult seminal vesicle proliferated and retained its normal functional activity. Thus, seminal vesicle functional cytodifferentiation can be faithfully reproduced in homotypic tissue recombinants. The methods used in this study will be used to investigate seminal vesicle development in instructive inductions of heterotypic epithelia.

scholarly journals Effect of neonatal chronic stress on expression of Hsp70 and oestrogen receptor alpha in the rat oviduct during development and the oestrous cycle

Reproduction ◽  
2003 ◽  
pp. 801-808 ◽  
Author(s):  
ML Mariani ◽  
DR Ciocca ◽  
AS Gonzalez Jatuff ◽  
M Souto
Keyword(s):  
Chronic Stress ◽  
Oestrogen Receptor ◽  
Depressive Disorders ◽  
Adult Life ◽  
Oestrous Cycle ◽  
Stress Model ◽  
Neonatal Development ◽  
Adult Rats ◽  
Receptor Alpha ◽  
Oestrogen Receptor Alpha

A chronic unpredictable stress model used to produce depressive disorders in adult rats was applied to neonatal rats to investigate whether this type of stress can induce changes in the expression of Hsp70 and oestrogen receptor alpha in the oviduct, as detected by immunohistochemistry. Rats stressed during neonatal development showed changes in the expression pattern of Hsp70. In neonatal control rats, Hsp70-positive cells observed in the isthmus did not show any changes. Moreover, rats exposed to this stress model that reached adulthood had higher expression of Hsp70 in the isthmus (P<0.01) but not in the ampulla during oestrus than did the control rats. In contrast, during dioestrus, no significant changes were noted in adult rats that were stressed during neonatal development or in rats that were stressed in adulthood. These findings indicate that the isthmus is very sensitive to stressful stimuli and that repeated pre-weaning stress can change the expression of heat shock proteins in early and adult life. These subtle changes of expression in the oviduct did not affect the fertility of the rats that reached adulthood or that were mated under unstressed conditions. However, the control animals stressed during adulthood showed a disruption of the oestrous cycle: this finding is not observed in rats stressed during neonatal development that show an attenuated oestrous cycle disruption induced by chronic stress in adulthood. Moreover, there was dissociation between the expression of oestrogen receptor alpha and Hsp70. The amount of oestrogen receptor alpha remained constant in the epithelium of the oviduct in the control and in the stressed rats. Expression of oestrogen receptor alpha was noted in the stroma of the oviduct without the concomitant expression of Hsp70. It is possible that in certain cells and tissues Hsp70 is not necessary for oestrogen receptor alpha to be functional or Hsp70 might be present at very low amounts but is sufficient for the receptor to function.

scholarly journals Heterogenous Turnover of Sperm and Seminal Vesicle Proteins in the Mouse Revealed by Dynamic Metabolic Labeling

2012 ◽  
Vol 11 (6) ◽  
pp. M111.014993 ◽  
Author(s):  
Amy J. Claydon ◽  
Steven A. Ramm ◽  
Andrea Pennington ◽  
Jane L. Hurst ◽  
Paula Stockley ◽  
...  
Keyword(s):  
Seminal Vesicle ◽  
Metabolic Labeling ◽  
Vesicle Proteins

scholarly journals CHRONIC ALCOHOLISM ON THE SEMINAL VESICLE AND TESTIS WEIGHT OF ADULT RATS (Rattus norvegicus)

1997 ◽  
Vol 15 (2) ◽  
Author(s):  
F. E. Martinez ◽  
M. Martinez ◽  
V. H. A. Cagnon ◽  
W. Mello Junior ◽  
C. R. Padovani ◽  
...  
Keyword(s):  
Seminal Vesicle ◽  
Rattus Norvegicus ◽  
Chronic Alcoholism ◽  
Testis Weight ◽  
Adult Rats

scholarly journals Deletion of a Seminal Gene Cluster Reinforces a Crucial Role of SVS2 in Male Fertility

2019 ◽  
Vol 20 (18) ◽  
pp. 4557 ◽  
Author(s):  
Miyuki Shindo ◽  
Masafumi Inui ◽  
Woojin Kang ◽  
Moe Tamano ◽  
Cai Tingwei ◽  
...  
Keyword(s):  
Seminal Vesicle ◽  
Gene Cluster ◽  
Loxp Site ◽  
Male Mice ◽  
Internal Fertilization ◽  
Immune Attack ◽  
Sperm Membrane ◽  
Encoding Gene ◽  
And Function ◽  
Vesicle Proteins

Multiple genes, whose functions or expression are overlapping, compensate for the loss of one gene. A gene cluster in the mouse genome encodes five seminal vesicle proteins (SVS2, SVS3, SVS4, SVS5, and SVS6). These proteins are produced by male rodents and function in formation of the copulatory plug following mating. SVS2 plays an essential role in the successful internal fertilization by protecting the sperm membrane against a uterine immune attack. We hypothesized that the four remaining seminal vesicle proteins (SVPs) of this gene cluster may partially/completely compensate for the deficiency of SVS2. For confirming our hypothesis, we generated mice lacking the entire SVP-encoding gene cluster and compared their fecundity with Svs2-deficient (Svs2−/−) mice; that is, mice deficient in Svs2 alone. A single loxP site remained after the deletion of the Svs2 gene. Therefore, we inserted another loxP site by combining the CRISPR/Cas9 system with single-stranded oligodeoxynucleotides (ssODN). Male mice lacking the entire SVP-encoding gene cluster (Svs2–6−/− mice) and thereby all five SVP proteins, generated by the deletion of 100kbp genomic DNA, showed low fecundity. However, the fecundity level was comparable with that from Svs2−/− male mice. Our results demonstrate that SVS3, SVS4, SVS5, and SVS6 do not function in the protection of sperm against a uterine immune attack in the absence of SVS2. Thus, Svs2 is the critical gene in the SVP gene cluster.

Svp-3, a third polymorphic locus for mouse seminal vesicle proteins

1983 ◽  
Vol 21 (7-8) ◽  
pp. 797-800 ◽  
Author(s):  
R. Moutier ◽  
M. F. Bertrand
Keyword(s):  
Seminal Vesicle ◽  
Polymorphic Locus ◽  
Vesicle Proteins

Neonatal chronic stress induces subsensitivity to chronic stress in adult rats: II. Effects on estrous cycle in females

1994 ◽  
Vol 56 (3) ◽  
pp. 591-595 ◽  
Author(s):  
A.S. González ◽  
E.L. Rodríguez Echandía ◽  
R. Cabrera ◽  
M.R. Fóscolo
Keyword(s):  
Estrous Cycle ◽  
Chronic Stress ◽  
Adult Rats
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