Gonadotropins affectOct-4 gene expression during mouse oocyte growth

2006 ◽  
Vol 73 (6) ◽  
pp. 685-691 ◽  
Author(s):  
Manuela Monti ◽  
Silvia Garagna ◽  
CarloAlberto Redi ◽  
Maurizio Zuccotti
Keyword(s):  
Gene Expression ◽  
Mouse Oocyte ◽  
Oocyte Growth

scholarly journals Gene Expression and Chromatin Organization during Mouse Oocyte Growth

1999 ◽  
Vol 207 (1) ◽  
pp. 76-85 ◽  
Author(s):  
Elisabeth Christians ◽  
Michele Boiani ◽  
Silvia Garagna ◽  
Cécile Dessy ◽  
Carlo Alberto Redi ◽  
...  
Keyword(s):  
Gene Expression ◽  
Chromatin Organization ◽  
Mouse Oocyte ◽  
Oocyte Growth

Genome-wide reprogramming by DNA demethylation during mouse oocyte growth and early development

2014 ◽  
Author(s):  
Akihiko Sakashita ◽  
Yosuke Iseki ◽  
Mei Nakajima ◽  
Takuya Wakai ◽  
Hisato Kobayashi ◽  
...  
Keyword(s):  
Early Development ◽  
Dna Demethylation ◽  
Mouse Oocyte ◽  
Oocyte Growth ◽  
Genome Wide

Laminin chain-specific gene expression during mouse oocyte maturation

1997 ◽  
Vol 48 (2) ◽  
pp. 185-193 ◽  
Author(s):  
Chanseob Shim ◽  
Sang Gu Lee ◽  
Woo Keun Song ◽  
Chul Sang Lee ◽  
Kyung-Kwang Lee ◽  
...  
Keyword(s):  
Gene Expression ◽  
Oocyte Maturation ◽  
Mouse Oocyte ◽  
Specific Gene ◽  
Specific Gene Expression

Gene expression during mammalian oogenesis and early embryogenesis: quantification of three messenger RNAs abundant in fully grown mouse oocytes

Development ◽  
1989 ◽  
Vol 106 (2) ◽  
pp. 251-261 ◽  
Author(s):  
R.J. Roller ◽  
R.A. Kinloch ◽  
B.Y. Hiraoka ◽  
S.S. Li ◽  
P.M. Wassarman
Keyword(s):  
Gene Expression ◽  
Steady State ◽  
Early Embryogenesis ◽  
Messenger Rnas ◽  
Oocyte Growth ◽  
Mouse Oocytes ◽  
Mouse Tissues ◽  
Specific Mrnas ◽  
Steady State Levels ◽  
Ribonuclease Protection

Ribonuclease protection assays have been used to quantitatively assess changes in steady-state levels of specific mRNAs during oogenesis and early embryogenesis in mice. The mRNAs encode ZP3 (a glycoprotein that serves as a sperm receptor), LDH-B (heart-type lactate dehydrogenase), and MOM-1 (a protein of unknown function). MOM-1 and LDH-B are expressed in a variety of adult mouse tissues and midgestation embryos, whereas ZP3 expression is restricted completely to oocytes. All three mRNAs are expressed by growing mouse oocytes and accumulate to unusually high levels in fully grown oocytes as compared to somatic cells; 240,000, 200,000 and 74,000 copies mRNA per fully grown oocyte for ZP3, LDH-B and MOM-1, respectively. Steady-state levels of LDH-B and MOM-1 mRNA undergo a modest decline (approximately 20–40%) during ovulation when fully grown oocytes become unfertilized eggs and, in general, mirror the reported change in poly(A)+RNA levels during this period of development. On the other hand, the level of ZP3 mRNA declines dramatically (approximately 98%) during ovulation, from approximately 240,000 copies per oocyte to approximately 5000 copies per unfertilized egg, and ZP3 mRNA is undetectable in fertilized eggs (less than 1000 copies per fertilized egg). MOM-1 mRNA is expressed at relatively low levels in morulae (approximately 2000 copies per embryo) and blastocysts (approximately 5000 copies per embryo), whereas ZP3 mRNA remains undetectable (less than 1000 copies per embryo) at these stages of preimplantation development. These findings are discussed in the context of overall gene expression during oocyte growth, meiotic maturation and early embryogenesis in mice.

Chromatin organization during mouse oocyte growth

1995 ◽  
Vol 41 (4) ◽  
pp. 479-485 ◽  
Author(s):  
Maurizio Zuccotti ◽  
Anna Piccinelli ◽  
Paolo Giorgi Rossi ◽  
Silvia Garagna ◽  
Carlo Alberto Redi
Keyword(s):  
Chromatin Organization ◽  
Mouse Oocyte ◽  
Oocyte Growth

scholarly journals An Accumulation of p34cdc2at the End of Mouse Oocyte Growth Correlates with the Acquisition of Meiotic Competence

1996 ◽  
Vol 174 (2) ◽  
pp. 335-344 ◽  
Author(s):  
C. de Vantéry ◽  
A.C. Gavin ◽  
J.D. Vassalli ◽  
S. Schorderet-Slatkine
Keyword(s):  
Mouse Oocyte ◽  
Oocyte Growth ◽  
Meiotic Competence

scholarly journals A Novel Biological Role of Tachykinins as an Up-Regulator of Oocyte Growth: Identification of an Evolutionary Origin of Tachykininergic Functions in the Ovary of the Ascidian, Ciona intestinalis

Endocrinology ◽  
2008 ◽  
Vol 149 (9) ◽  
pp. 4346-4356 ◽  
Author(s):  
Masato Aoyama ◽  
Tsuyoshi Kawada ◽  
Manabu Fujie ◽  
Kohji Hotta ◽  
Tsubasa Sakai ◽  
...  
Keyword(s):  
Gene Expression ◽  
Regulation Of Gene Expression ◽  
Model Organism ◽  
Ciona Intestinalis ◽  
Enzymatic Activities ◽  
Evolutionary Origin ◽  
Phylogenetic Position ◽  
Specific Expression ◽  
Oocyte Growth ◽  
Vitellogenic Stage

Tachykinins (TKs) and their receptors have been shown to be expressed in the mammalian ovary. However, the biological roles of ovarian TKs have yet to be verified. Ci-TK-I and Ci-TK-R, characterized from the protochordate (ascidian), Ciona intestinalis, are prototypes of vertebrate TKs and their receptors. In the present study, we show a novel biological function of TKs as an inducible factor for oocyte growth using C. intestinalis as a model organism. Immunostaining demonstrated the specific expression of Ci-TK-R in test cells residing in oocytes at the vitellogenic stage. DNA microarray and real-time PCR revealed that Ci-TK-I induced gene expression of several proteases, including cathepsin D, chymotrypsin, and carboxy-peptidase B1, in the ovary. The enzymatic activities of these proteases in the ovary were also shown to be enhanced by Ci-TK-I. Of particular significance is that the treatment of Ciona oocytes with Ci-TK-I resulted in progression of growth from the vitellogenic stage to the post-vitellogenic stage. The Ci-TK-I-induced oocyte growth was blocked by a TK antagonist or by protease inhibitors. These results led to the conclusion that Ci-TK-I enhances growth of the vitellogenic oocytes via up-regulation of gene expression and enzymatic activities of the proteases. This is the first clarification of the biological roles of TKs in the ovary and the underlying essential molecular mechanism. Furthermore, considering the phylogenetic position of ascidians as basal chordates, we suggest that the novel TK-regulated oocyte growth is an “evolutionary origin” of the tachykininergic functions in the ovary.

scholarly journals Alterations in epigenetic modifications during oocyte growth in mice

Reproduction ◽  
2007 ◽  
Vol 133 (1) ◽  
pp. 85-94 ◽  
Author(s):  
Shun-ichiro Kageyama ◽  
Honglin Liu ◽  
Naoto Kaneko ◽  
Masatoshi Ooga ◽  
Masao Nagata ◽  
...  
Keyword(s):  
Gene Expression ◽  
Histone Modifications ◽  
Epigenetic Modification ◽  
Germinal Vesicle ◽  
Epigenetic Modifications ◽  
Condensed Chromatin ◽  
Rt Pcr ◽  
Oocyte Growth ◽  
Lysine Residues ◽  
Signal Intensities

During oocyte growth, chromatin structure is altered globally and gene expression is silenced. To investigate the involvement of epigenetic modifications in the regulation of these phenomena, changes in global DNA methylation and in various histone modifications, i.e. acetylation of H3K9, H3K18, H4K5, and H4K12, and methylation of H3K4 and H3K9, were examined during the growth of mouse oocytes. Immunocytochemical analysis revealed that the signal intensities of all these modifications increased during growth and that fully grown, germinal vesicle (GV)-stage oocytes showed the most modifications. Since acetylation of most of the lysine residues on histones and methylation of H3K4 are associated with active gene expression, the increased levels of these modifications do not seem to be associated with gene silencing in GV-stage oocytes. Given that there are two types of GV-stage oocytes with different chromatin configurations and transcriptional activities, the epigenetic modification statuses of these two types were compared. The levels of all the epigenetic modifications examined were higher in the SN(surrounded nucleolus)-type oocytes, in which highly condensed chromatin is concentrated in the area around the nucleolus and gene expression is silenced than in the NSN(not surrounded nucleolus)-type oocytes, in which less-condensed chromatin does not surround the nucleolus and gene expression is active. In addition, the expression levels of various enzymes that catalyze histone modifications were shown by RT-PCR to increase with oocyte growth. Taken together, the results show that all of the epigenetic modifications increased in a concerted manner during oocyte growth, and suggest that these increases are not associated with gene expression.

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