scholarly journals An Accumulation of p34cdc2at the End of Mouse Oocyte Growth Correlates with the Acquisition of Meiotic Competence

1996 ◽  
Vol 174 (2) ◽  
pp. 335-344 ◽  
Author(s):  
C. de Vantéry ◽  
A.C. Gavin ◽  
J.D. Vassalli ◽  
S. Schorderet-Slatkine
Keyword(s):  
Mouse Oocyte ◽  
Oocyte Growth ◽  
Meiotic Competence

Genome-wide reprogramming by DNA demethylation during mouse oocyte growth and early development

2014 ◽  
Author(s):  
Akihiko Sakashita ◽  
Yosuke Iseki ◽  
Mei Nakajima ◽  
Takuya Wakai ◽  
Hisato Kobayashi ◽  
...  
Keyword(s):  
Early Development ◽  
Dna Demethylation ◽  
Mouse Oocyte ◽  
Oocyte Growth ◽  
Genome Wide

Chromatin organization during mouse oocyte growth

1995 ◽  
Vol 41 (4) ◽  
pp. 479-485 ◽  
Author(s):  
Maurizio Zuccotti ◽  
Anna Piccinelli ◽  
Paolo Giorgi Rossi ◽  
Silvia Garagna ◽  
Carlo Alberto Redi
Keyword(s):  
Chromatin Organization ◽  
Mouse Oocyte ◽  
Oocyte Growth

Role of Intercellular Coupling on Chromatin Changes Transcriptional Activity and Meiotic Competence Acquisition During Bovine Oocyte Growth In Vitro.

2009 ◽  
Vol 81 (Suppl_1) ◽  
pp. 282-282
Author(s):  
Federica M. Franciosi ◽  
Valentina Lodde ◽  
Silvia Modina ◽  
Irene Tessaro ◽  
Alberto M. Luciano
Keyword(s):  
Transcriptional Activity ◽  
Bovine Oocyte ◽  
Intercellular Coupling ◽  
Oocyte Growth ◽  
Competence Acquisition ◽  
Meiotic Competence

scholarly journals Expression of c-MYC in Nuclear Speckles During Mouse Oocyte Growth and Preimplantation Development

2009 ◽  
Vol 55 (5) ◽  
pp. 491-495 ◽  
Author(s):  
Tsukasa SUZUKI ◽  
Ken-ichiro ABE ◽  
Azusa INOUE ◽  
Fugaku AOKI
Keyword(s):  
Preimplantation Development ◽  
Mouse Oocyte ◽  
Nuclear Speckles ◽  
Oocyte Growth

Electrical maturation of the murine oocyte: an increase in calcium current coincides withacquisition of meiotic competence

Zygote ◽  
1993 ◽  
Vol 1 (1) ◽  
pp. 49-60 ◽  
Author(s):  
Joan M. Murnane ◽  
Louis J. DeFelice
Keyword(s):  
Polar Body ◽  
Reversal Potential ◽  
Germinal Vesicle ◽  
Outward Current ◽  
Ca Channels ◽  
Oocyte Growth ◽  
Inward Current ◽  
First Polar Body ◽  
Meiotic Competence

SummaryWe have used the whole-cell recording technique to compare three stages of primary and secondary oocytes from F1 hybrid mice (C57BL/6J x SJL/J):neonatal germinal vesicle (NGV) stage primary oocytes from 10- to 20-day-old, prepubescent mice; mature germinal vesicle (MGV) stage primary oocytes from 12-week-old, post-pubescent, superovulated mice; first polar body (FPB) stage secondary oocytes from 12-week-old, post-pubescent mice during the normal oestrus cycle or following superovulation. NGV, MGV and FPB oocytes all exhibit two voltage-dependent currents: an inward, rapidly activating/inactivating current, and an outward, slowly activating/non-inactivating current. In 1.5 mmol/1 external Ca the average peak inward current is − 2.9, − 12.4 and − 13.8 μA/cm2 in NGV, MGV and FPB oocytes, respectively. In 20 mmol/1 Ca these currents increase and the reversal potential shifts to the right. The outward current decreases slightly with growth and development: at 40 mV test potentials, NGV oocytes have average outward currents of 8.9 μA/cm2, and MGV and FPB oocytes have currents of 5.0 and 5.5 μA/cm2, respectively. Thus, MGV oocytes express FPB current patterns. The reversal potentials, kinetics and pharmacology of the currents indicate that Ca channels carry the inward current and K channels carry the outward current. During growth in vivo a gradual depolarisation accompanies maturation. Resting potentialsranged from − 45 to − 30 mV in NGV oocytes to − 35 to − 17 mV in MGV oocytes to − 20 mV to − 3 mV in FPB oocytes. These data suggest that a selective increase occurs in the number of Ca channels during oocyte growth. This increase precedes nuclear maturation and coincides with the acquisition of meiotic competence.

186 EFFECT OF POLYVINYLPYRROLIDONE SUPPLEMENTATION IN CULTURE MEDIUM ON THE GROWTH OF MOUSE OOCYTES

2013 ◽  
Vol 25 (1) ◽  
pp. 242
Author(s):  
S. Mizumachi ◽  
K. Sasaki ◽  
K. Matsubara ◽  
Y. Hirao
Keyword(s):  
Granulosa Cell ◽  
Culture Medium ◽  
Polar Body ◽  
Cumulus Cell ◽  
Mouse Oocyte ◽  
Oocyte Diameter ◽  
Oocyte Growth ◽  
Cell Complexes ◽  
Bovine Oocytes

A high volume of polyvinylpyrrolidone (PVP) supplementation in culture medium has a significant impact on the growth of bovine oocytes. The objective of the present study was to determine whether or not PVP affects oocyte growth in the mouse. Oocyte–granulosa cell complexes were isolated from 11- or 12-day-old mice (ICR) by mechanical isolation of follicles, followed by a collagenase treatment (0.1%; 10 min). Twenty complexes were placed on each insert fit in the 24-well culture plate and cultured for 10 days in an atmosphere of 5% CO2 in air at 37°C. The culture medium was a modified α-MEM supplemented with 5% fetal bovine serum and 1 ng mL–1 FSH. The concentration of PVP (molecular weight of 360 000) was 0%, 1%, 2%, or 3% (w/v). During the first 2 days, only medium with 0% PVP was used. The oocytes recovered on Day 10 were subjected to in vitro maturation, IVF, and embryo culture. In 12 replications, the total numbers of oocytes cultured in medium with 0%, 1%, 2%, and 3% PVP were 235, 233, 233, and 231, respectively. In some additional experiments, oocytes were fixed on Day 10 and processed for transmission electron microscopy (TEM). The oocytes in medium with 0% PVP became located within an enlarged dome-like structure. In medium with 2% PVP and 3% PVP, no such domes were formed, and the oocytes within several granulosa cell layers were exposed to medium; however, the cumulus cell mass specifically became larger than that in medium with 0% PVP. The viabilities of oocytes recovered from medium with 0%, 1%, 2%, and 3% PVP were 83%, 81%, 91%, and 93%, respectively. The survival rate was significantly higher in medium with 3% PVP than in medium with 0% PVP or 1% PVP (P < 0.05). The mean oocyte diameter increased from 59 µm (Day 0) to 72, 71, 71, and 72 µm in medium with 0, 1, 2, and 3% PVP, respectively, but they continued to be smaller than in vivo grown oocytes (81.0 µm; P < 0.01). When maturation was induced, cumulus cell mucification occurred irrespective of PVP concentration during the growth. No significant differences were found between the groups in the percentage of polar body extrusion (ranging from 78 to 88%). Developmental outcomes based on oocytes used for in vitro fertilization were the following: cleavage rates were 67, 78, 74, and 76%; and blastocyst rates were 37, 44, 47, and 36% of oocytes that had been grown in medium with 0, 1, 2, and 3% PVP, respectively. The numbers of oocytes included were 60, 59, 68, and 66, respectively. The TEM observation suggests that more intimate contacts were maintained between the oocyte and cumulus cells in medium with 2% PVP than in medium with 0% PVP. Taken together, PVP supplementation in medium has a considerable influence on the morphology of mouse oocyte–granulosa cell complexes and close contacts within the complexes in the long-term culture, as having been observed with bovine oocytes.

Gonadotropins affectOct-4 gene expression during mouse oocyte growth

2006 ◽  
Vol 73 (6) ◽  
pp. 685-691 ◽  
Author(s):  
Manuela Monti ◽  
Silvia Garagna ◽  
CarloAlberto Redi ◽  
Maurizio Zuccotti
Keyword(s):  
Gene Expression ◽  
Mouse Oocyte ◽  
Oocyte Growth

scholarly journals Expression and localization of nitric oxide synthase isoforms during porcine oocyte growth and acquisition of meiotic competence

2009 ◽  
Vol 54 (No. 4) ◽  
pp. 137-149 ◽  
Author(s):  
E. Chmelíková ◽  
M. Sedmíková ◽  
J. Petr ◽  
T. Kott ◽  
V. Lánská ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Nitric Oxide Synthase ◽  
Intracellular Localization ◽  
Growth Period ◽  
Fluorescence Signal ◽  
Oocyte Growth ◽  
Porcine Oocyte ◽  
Oxide Synthase ◽  
Nos Isoforms ◽  
Meiotic Competence

Reproduction biotechnologies depend on the use of fully meiotically competent oocytes. Growing oocytes without full meiotic competence are an interesting potential source due to their quantity, but the mechanisms regulating the processes of acquisition of meiotic competence have not been clarified to date. Nitric oxide synthase (NOS) and its product, nitric oxide (NO), may possibly play a role. Understanding the precise NO regulatory mechanism is therefore important for the development of <I>in vitro</I> growth methods. The objective of this work was to detect changes in the expression of NOS isoforms and their mRNA expression and changes in the intracellular localization of separate NOS isoforms during the growth period of the porcine oocyte, and also to determine whether these changes are related to the process of meiotic competence acquisition. mRNA for all NOS isoforms was already detected in oocytes at the beginning of their growth and was present in them until they completed their growth period. mRNA for iNOS and eNOS was also observed in granulosa and cumulus cells from these oocytes. But nNOS mRNA was not demonstrated in these types of cells. Pig oocytes and their surrounding cells contained all NOS proteins. Their amounts increased and localization changed with the acquisition of meiotic competence. nNOS was localized mainly in the cortex in meiotically incompetent oocytes, while meiotically competent oocytes contained nNOS in the nucleus as well. iNOS protein was distributed in the cytoplasm and nucleus in all oocytes, and meiotically incompetent oocytes contained iNOS in the nucleolus as well. eNOS protein was distributed in oocytes in the form of fine granules with a strong fluorescence signal. Protein was concentrated in the nuclear area in meiotically incompetent oocytes and also in the periphery in oocytes with partially and fully-developed meiotic competence. All these findings indicate that NOS isoforms may significantly influence the acquisition of meiotic competence in porcine oocytes.

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