Determination of bioaccessibility of β-carotene in vegetables by in vitro methods

2006 ◽  
Vol 50 (11) ◽  
pp. 1047-1052 ◽  
Author(s):  
Supriya Veda ◽  
Akshaya Kamath ◽  
Kalpana Platel ◽  
Khyrunnisa Begum ◽  
Krishnapura Srinivasan
Keyword(s):  
In Vitro Methods ◽  
Β Carotene

scholarly journals Deschampsia antarctica E. Desv. plants with different chromosome number cultivated in vitro. Relations between genome size and two adaptability indices

1970 ◽  
pp. 304-309
Author(s):  
I. Yu. Parnikoza ◽  
N. Yu. Miryuta ◽  
M. Roiek ◽  
A. A. Betiekhtin ◽  
O. O. Poronnik ◽  
...  
Keyword(s):  
Genome Size ◽  
Leaf Length ◽  
Cultivation Condition ◽  
Deschampsia Antarctica ◽  
Flow Cytometry Analysis ◽  
Plant Leaves ◽  
Biochemical Method ◽  
In Vitro Methods

Aim. We have researched probabilistic relations between genome size and two adaptability indices in eleven genotypes revealed in seeds germ of Deschampsia antarctica E. Desv. which has different genome size under standardized cultivation condition in vitro. Methods. We have used biochemical method for determination of flavonoid contents in plant leaves and flow cytometry analysis for determination of genome size in plant leaves. Results. Eleven genotypes of Deschampsia antarctica with different genome size have been researched by three data rows. Probabilistic scheme have been created and analyzed for all researched genotypes. Conclusions. The Deschampsia antarctica genome size or karyotype changes have been shown to have effect on its probabilistic relations with flavonoids content and leaf length. Mechanisms of such relations is required a detailed research. Keywords: Deschampsia antarctica, genome size, leaf length, flavonoids.

scholarly journals Phytochemical composition and biological screening of two Lophophytum species

2021 ◽  
Vol 20 (6) ◽  
pp. 598-610
Author(s):  
Carola A. Torres ◽  
◽  
Cristina M. Perez Zamora ◽  
Hector A. Sato ◽  
Maria B. Nuñez ◽  
...  
Keyword(s):  
Chemical Composition ◽  
In Vivo Studies ◽  
Tree Roots ◽  
In Vitro Methods ◽  
Phytochemical Composition ◽  
First Results ◽  
Anti Inflammatory ◽  
Β Carotene

Lophophytum species are holoparasites that grow on tree roots. The objectives of the work were to explore the chemical composition of the tubers of two Lophophytum species and to analyze the antioxidant, anti-inflammatory and antilithiatic activity of their extracts using in vitro methods. The chemical composition was determined by histochemical, phytochemical and TLC tests. In addition, the profile of phenolic compounds was determined by HPLC-MS. The presence of secondary metabolites of recognized activity was demonstrated. The results of the HPLC-MS/MS allowed the tentative identification of catechin, luteolin and glycosides of eriodictyol, naringenin and luteolin in the extract of Lophophytum leandri and eriodictyol, naringenin, luteolin and their glycosylated derivatives in Lophophytum mirabile. The extracts showed promising antioxidant (DPPH, ABTS and β-carotene-linoleic acid), anti-inflammatory (inhibition of 5-LOX) and anti-urolytic (by bioautographic TLC) activity. It is noteworthy that these are the first results of the phytochemical composition and biological activity of L. mirabile. However, in vivo studies are required to corroborate these activities.

Standardization of in-vitro methods for determination of antimicrobial properties of essential oils

2021 ◽  
Vol Publish Ahead of Print ◽  
Author(s):  
Iraj Rasooli ◽  
Horieh Saderi ◽  
Reza MohammadSalehi ◽  
Masoud Dadashi ◽  
Parviz Owlia
Keyword(s):  
Essential Oils ◽  
Antimicrobial Properties ◽  
In Vitro Methods

scholarly journals Deschampsia antarctica E. Desv. plants with different chromosome number cultivated in vitro. Probabilistic relations of three adaptability indices with genome size

1970 ◽  
pp. 293-298
Author(s):  
N. Yu. Miryuta ◽  
I. Yu. Parnikoza ◽  
O. O. Poronnik ◽  
G. Yu. Myryuta ◽  
V. A. Kunakh
Keyword(s):  
Genome Size ◽  
Leaf Length ◽  
Cultivation Condition ◽  
Deschampsia Antarctica ◽  
Plant Leaves ◽  
Electrophoretic Method ◽  
In Vitro Methods ◽  
Special Information

Aim. This work deals with probabilistic relations between genome size and adaptability indices research in 11 genotypes plants revealed among seeds of Deschampsia antarctica E. Desv. which has different genome size under standardized cultivation condition in vitro. Methods. We have used electrophoretic method for determination of protein relative contents in plant leaves, and statistical analysis for probabilistic relations determination. Results. Protein densitometric profiles for 11 D. antarctica genotypes plants were analyzed. Probabilistic scheme have been created for all researched plants genotypes for all previously analyzed parameters. Three probabilistic schemes of genotypes plants received from seeds collected in the same loci and season have been shown and analyzed. Conclusions. Protective protein densitometric profiles were similar for 11 genotypes of D. antarctica plants under standardized cultivation condition in vitro but quantitative part of protective protein has been changed. Genome size had an influence on its probabilistic relations with flavonoids content, leaf length and some protein relative contents in general pool. Probabilistic relations between genome size and some protein relative content and between the last and flavonoids content and leaf length is assumed to express underlying in this plants adaptation process to uniqueness nature condition in dynamic hereditary memory special information in certain degree. Mechanisms of probabilistic relations changes are required a detailed research.Keywords: Deschampsia antarctica, plants in vitro, probabilistic relations between genome size and adaptability indices, plant adaptability.

scholarly journals Mycotoxin bioaccessibility/absorption assessment using in vitro digestion models: a review

2013 ◽  
Vol 6 (2) ◽  
pp. 167-184 ◽  
Author(s):  
C.A. González-Arias ◽  
S. Marín ◽  
V. Sanchis ◽  
A.J. Ramos
Keyword(s):  
Animal Studies ◽  
In Vitro Digestion ◽  
Contamination Level ◽  
Food Matrix ◽  
Gi Tract ◽  
Cell Culture Techniques ◽  
In Vitro Methods

In the evaluation of the oral bioavailability of a mycotoxin, the first step is the determination of its bioaccessibility, i.e. the percentage of mycotoxin released from the food matrix during digestion in the gastrointestinal (GI) tract that could be absorbed through the intestinal epithelium. Different in vitro digestion models have been recently used for determination of bioaccessibility, thereby avoiding the use of more complex cell culture techniques or the use of animals in expensive in vivo experiments. In vitro methods offer an appealing alternative to human and animal studies. They usually are rapid, simple and reasonably low in cost, and can be used to perform simplified experiments under uniform and well-controlled conditions, providing insights not achievable in whole animal studies. The available in vitro methods for GI simulation differ in the design of the system, the composition of the physiological juices assayed, as well as in the use or not of intestinal microbiota. There are models that only simulate the upper part of the GI tract (mouth-stomach-small intestine), whereas other methods include the large intestine, so that the model chosen could have some influence on the bioaccessibility data obtained. Bioaccessibility depends on the food matrix, as well as on the contamination level and the way the food/feed is contaminated (spiked or naturally). This review focuses on the currently available data regarding in vitro digestion models for the study of the bioaccessibility or absorption of mycotoxins, detailing the characteristics of each digestion step and the importance of the physiological juices employed during digestion. The effect that different factors play on mycotoxin release from the food matrix in the GI tract is also considered, and existing data on bioaccessibility of the main mycotoxins are given.

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