Determination of β-Carotene and Lutein Available from Green Leafy Vegetables by an in Vitro Digestion and Colonic Fermentation Method

2005 ◽  
Vol 53 (8) ◽  
pp. 2936-2940 ◽  
Author(s):  
José Serrano ◽  
Isabel Goñi ◽  
Fulgencio Saura-Calixto
Keyword(s):  
In Vitro Digestion ◽  
Leafy Vegetables ◽  
Colonic Fermentation ◽  
Green Leafy Vegetables ◽  
Fermentation Method ◽  
Β Carotene

scholarly journals Nutrient and Total Polyphenol Contents of Dark Green Leafy Vegetables, and Estimation of Their Iron Bioaccessibility Using the In Vitro Digestion/Caco-2 Cell Model

Foods ◽  
2017 ◽  
Vol 6 (7) ◽  
pp. 54 ◽  
Author(s):  
Francis Amagloh ◽  
Richard Atuna ◽  
Richard McBride ◽  
Edward Carey ◽  
Tatiana Christides
Keyword(s):  
Cell Model ◽  
In Vitro Digestion ◽  
Leafy Vegetables ◽  
Total Polyphenol ◽  
Green Leafy Vegetables ◽  
Dark Green

scholarly journals Diversity in the Bioaccessibility of Carotenoid and Chlorophyll Compounds in 69 Spinach Genotypes (P06-007-19)

2019 ◽  
Vol 3 (Supplement_1) ◽  
Author(s):  
Micaela Hayes ◽  
Marti Pottorff ◽  
Colin Kay ◽  
Allen Van Deynze ◽  
Jualiana Osorio-Marin ◽  
...  
Keyword(s):  
In Vitro Digestion ◽  
Leafy Vegetables ◽  
Research Station ◽  
Pheophytin A ◽  
Factors Affecting ◽  
Green Leafy Vegetables ◽  
Resource Information ◽  
Germplasm Collections ◽  
Food And Agriculture

Abstract Objectives Spinach is one of the most nutrient dense and popular green leafy vegetables. Known as a rich source of pro-vitamin A carotenoids and chlorophylls, the variation in content of these bioactives has been associated with differences in genetics, environment and processing. However, beyond processing, factors affecting their bioavailability remain relatively unknown. Establishing the presence of diversity in bioaccessibility, a phenotypical surrogate for bioavailability, of phytochemicals from public germplasm collections and commercial cultivars would provide critical information to breeders seeking to improve the nutritional value of the material used in their programs. Methods 69 spinach accessions from both the USDA Germplasm Resource Information Network (GRIN) and the Center for Genetic Resources (CGN), the Netherlands, and selected commercial varieties were greenhouse-grown during the Fall 2018 season at Piedmont Research Station, NC, harvested, washed, blanched (100°C, 2 min) and homogenized (30 sec). Carotenoid and chlorophyll bioaccessibility were determined from spinach homogenates formulated with 5% canola oil using a high-throughput, three-phase in vitro digestion followed by centrifugation and filtration to isolate the micellar fraction. Transfer of lutein (LUT), β-carotene (BC) and pheophytin A (Phe A) and B (Phe B) from spinach to micellar fractions was quantified by LC to determine bioaccessibility. Results LUT and BC were well recovered through in vitro digestion while chlorophylls were quantitatively converted to corresponding pheophytins. Relative bioaccessibility for all phytochemicals was found to be significantly different across genotypes, as determined by ANOVA analysis. LUT bioaccessibiltiy ranged from 9.4–30.6% (P = 0.01), BC (12.9–42.5%; P < 0.001), PheA (24.5–51%; P = < 0.001), and PheB (19.8–46.9% P = < 0.001). Absolute bioaccessible content ranged from 0.6–8.2 and 0.9–3.7 mg/100 g fw spinach for LUT and BC, respectively. Conclusions Results suggest that carotenoid and chlorophyll bioaccessibility may vary based on spinach genotype. The relationship of bioaccessibility to quality traits and potential interactions with agronomic (GxE) and processing (GxP) conditions remain to be explored. Funding Sources Foundation for Food and Agriculture Research.

scholarly journals EVALUATION OF DEHYDRATED MUKIA MADERASPATANA LEAVES AS A POTENT SOURCE OF DIETARY ANTIOXIDANTS

2019 ◽  
Vol 7 (6) ◽  
pp. 27-36
Author(s):  
D. Amirtham ◽  
J. Aswini Nivedida ◽  
K. Dhivya ◽  
S. Ganapathy ◽  
C. Indurani
Keyword(s):  
Phenolic Content ◽  
Antioxidant Activities ◽  
Ethanolic Extract ◽  
Antioxidant Property ◽  
Leafy Vegetables ◽  
Total Phenolic ◽  
Green Leafy Vegetables ◽  
Total Antioxidant ◽  
Indigenous Plant

Green leafy vegetables are the most underexploited class of vegetables despite high nutritional value. The current study has been focused on the evaluation of anti-oxidant status of fresh and dehydrated under- utilized green leafy vegetable namely Mukia maderaspatana (L.) (Family: Cucurbitaceae), an indigenous plant; traditionally it is used as an ingredient of various cocktail preparations for the management of severe inflammatory disorders in Indian system of medicine. The total phenolic content (TPC), total flavonoid content (TFC) and total antioxidant activities were evaluated for the ethanolic extract of leaves to assess the in vitro antioxidant activities. The results showed that there exist a linear correlation between polyphenol content and antioxidant property. The ethanolic extract of dehydrated Mukia leaves showed the highest phenolic content (269.34 ± 0.78mg GAE/g), and total antioxidant activity (543±46 µmol Trolox/100 g). HPTLC analysis has revealed the presence of significant quantity of Quercitin (26.52%), an important flavonoid of tremendous antioxidant, anticancer and ant inflammatory properties in both the fresh and dehydrated leaves which might be the chief bioactive principle in Mukia.

Development and Validation of an HPLC Method for the Determination of Thiamine and Riboflavin in Green Leafy Vegetables Using Clara-Diastase

2011 ◽  
Vol 76 (4) ◽  
pp. C639-C642 ◽  
Author(s):  
Alicia del Carmen Mondragón-Portocarrero ◽  
Lourdes Vázquez-Odériz ◽  
MaÁngeles Romero-Rodríguez
Keyword(s):  
Hplc Method ◽  
Leafy Vegetables ◽  
Green Leafy Vegetables ◽  
Development And Validation

β-carotene as an inhibitor of benzo(a)pyrene and mitomycin C induced chromosomal breaks in the bone marrow of mice

1985 ◽  
Vol 27 (5) ◽  
pp. 598-602 ◽  
Author(s):  
A. S. Raj ◽  
Morris Katz
Keyword(s):  
Bone Marrow ◽  
Mitomycin C ◽  
Micronucleus Assay ◽  
Leafy Vegetables ◽  
Hybrid Strain ◽  
Green Leafy Vegetables ◽  
Polychromatic Erythrocytes ◽  
Chromosomal Breaks ◽  
Β Carotene

Female mice of hybrid strain B6C3F1, 8–10 weeks old, were fed on powdered food with or without β-carotene (100 mg/kg food). After 1 week of these diets, some of each group of mice were injected i.p. with either benzo(a)pyrene (150 mg/kg) in dimethyl sulfoxide, or mitomycin C (1 mg/kg) in distilled water. In the course of separate experiments, bone marrow samples were collected at various intervals after injection for analysis in the in vivo bone marrow micronucleus assay. At the time at which the maximum induction was observed, which coincided between experiments, the frequency of micronuclei induced by benzo(a)pyrene was reduced by 41–61% and that induced by mitomycin C was reduced by 44–71% in the presence of β-carotene. β-carotene is widely distributed in plant material such as carrots and green leafy vegetables and, as such, is a component of the human diet. Our results suggest that β-carotene provides significant protection against the genotoxicity of benzo(a)pyrene and mitomycin C.Key words: β-carotene, inhibitor, chromosomal breaks, micronucleated polychromatic erythrocytes.

Evaluation of Cellular Absorption and Metabolism of β-Carotene Loaded in Nanocarriers after In Vitro Digestion

2021 ◽  
Author(s):  
Ling Chen ◽  
Wallace Yokoyama ◽  
Christina Tam ◽  
Yuqing Tan ◽  
Pricilla Alves ◽  
...  
Keyword(s):  
In Vitro Digestion ◽  
Β Carotene
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