scholarly journals W361R mutation in GaaR, the regulator of D‐galacturonic acid‐responsive genes, leads to constitutive production of pectinases inAspergillus niger

2018 ◽  
Vol 8 (5) ◽  
pp. e00732 ◽  
Author(s):  
Ebru Alazi ◽  
Jing Niu ◽  
Simon B. Otto ◽  
Mark Arentshorst ◽  
Thi T. M. Pham ◽  
...  
Keyword(s):  
Galacturonic Acid ◽  
Constitutive Production

Purdie reagent-induced C(5)-epimerization of D-galacturonic acid derivatives in the presence of methyl sulphide

1976 ◽  
Vol 41 (10) ◽  
pp. 3119-3130 ◽  
Author(s):  
P. Kováč ◽  
J. Hirsch ◽  
R. Palovčík ◽  
I. Tvaroška ◽  
S. Bystrický
Keyword(s):  
Galacturonic Acid

Influence of the Carboxylic Function on the Degradation of d-Galacturonic Acid and Its Polymers

2021 ◽  
Author(s):  
Alexandra Fatouros ◽  
Ulrike Einhorn-Stoll ◽  
Hanna Kastner ◽  
Stephan Drusch ◽  
Lothar W. Kroh
Keyword(s):  
Galacturonic Acid

scholarly journals Enzymatic Extraction and Characterization of Pectin from Cocoa Pod Husks (Theobroma cacao L.) Using Celluclast® 1.5 L

Molecules ◽  
2021 ◽  
Vol 26 (5) ◽  
pp. 1473
Author(s):  
Licelander Hennessey-Ramos ◽  
Walter Murillo-Arango ◽  
Juliana Vasco-Correa ◽  
Isabel Cristina Paz Astudillo
Keyword(s):  
Acid Content ◽  
Galacturonic Acid ◽  
Extraction Process ◽  
Chemical Extraction ◽  
Enzymatic Extraction ◽  
Acid Yield ◽  
Physicochemical Features ◽  
Enzyme Dosage ◽  
Cocoa Pod Husks

Cocoa pod husks are a waste generated during the processing of cocoa beans. We aimed to explore the enzymatic extraction of pectin using cellulases. The extraction process was optimized using a central composite design (CCD) and analyzed by response surface methodology (RSM). The parameters optimized were feedstock concentration (%), enzyme dosage (µL/g), and time (h). Three dependent variables were studied: pectin yield (g/100 g dry husk) (R2 = 97.02), galacturonic acid content (g/100 g pectin) (R2 = 96.90), and galacturonic acid yield (g/100 g feedstock) (R2 = 95.35). The optimal parameters were 6.0% feedstock concentration, 40 µL g−1 of enzyme, and 18.54 h, conditions that produced experimentally a pectin yield of 10.20 g/100 g feedstock, 52.06 g galacturonic acid/100 g pectin, and a yield 5.31 g galacturonic acid/100 g feedstock. Using the chemical extraction method, a yield of 8.08 g pectin/100 g feedstock and a galacturonic acid content of 60.97 g/100 g pectin were obtained. Using assisted sonication, a pectin yield of 8.28 g/100 g feedstock and a galacturonic acid content of 42.77 g/100 g pectin were obtained. Enzymatically optimized pectin has rheological and physicochemical features typical of this biomaterial, which provides an interesting alternative for the valorization of cocoa husks.

scholarly journals Small oligomers of galacturonic acid are endogenous suppressors of disease resistance reactions in wheat leaves

1999 ◽  
Vol 50 (334) ◽  
pp. 605-612 ◽  
Author(s):  
B. M. Moerschbacher ◽  
M. Mierau ◽  
B. Graessner ◽  
U. Noll ◽  
A. J. Mort
Keyword(s):  
Disease Resistance ◽  
Galacturonic Acid ◽  
Wheat Leaves

Regulation of 25-hydroxyvitamin D3-1α-hydroxylase and production of 1α,25-dihydroxyvitamin D3 by human dendritic cells

Blood ◽  
2003 ◽  
Vol 102 (9) ◽  
pp. 3314-3316 ◽  
Author(s):  
Jana Fritsche ◽  
Krishna Mondal ◽  
Achim Ehrnsperger ◽  
Reinhard Andreesen ◽  
Marina Kreutz
Keyword(s):  
Dendritic Cells ◽  
Early Phase ◽  
Constitutive Expression ◽  
Terminal Differentiation ◽  
Dihydroxyvitamin D3 ◽  
Key Enzyme ◽  
Functional Consequences ◽  
Constitutive Production ◽  
Human Dendritic Cells ◽  
First Time

Abstract25-Hydroxyvitamin D3-1α-hydroxylase (25(OH)D3-1α-hydroxylase), the key enzyme of 1α,25-dihydroxyvitamin D3 (1,25(OH)2D3) production, is expressed in monocyte-derived macrophages (MACs). Here we show for the first time constitutive expression of 25(OH)D3-1α-hydroxylase in monocyte-derived dendritic cells (DCs), which was increased after stimulation with lipopolysaccharide (LPS). Accordingly, DCs showed low constitutive production of 1,25(OH)2D3, but activation by LPS increased 1,25(OH)2D3 synthesis. In addition, 25(OH)D3-1α-hydroxylase expression was found in blood DCs but not in CD34+-derived DCs. Next we analyzed the functional consequences of these results. Addition of 1,25(OH)2D3 at concentrations comparable with those produced by DCs inhibited the allostimulatory potential of DCs during the early phase of DC differentiation. However, terminal differentiation decreased the responsiveness of DCs to 1,25(OH)2D3. In conclusion, DCs are able to produce 1,25(OH)2D3 especially following stimulation with LPS. Terminal maturation renders DCs unresponsive to the effects of 1,25(OH)2D3, but those cells are able to suppress the differentiation of their own precursor cells in a paracrine way through the production of 1,25(OH)2D3.

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