Mesenchymal stromal cells modulate tissue repair responses within the injured vocal fold

2019 ◽  
Vol 130 (1) ◽  
Author(s):  
Srinivasa Rao Nagubothu ◽  
Rachael V. Sugars ◽  
Nikolce Tudzarovski ◽  
Anton Törnqvist Andrén ◽  
Matteo Bottai ◽  
...  
Keyword(s):  
Mesenchymal Stromal Cells ◽  
Stromal Cells ◽  
Tissue Repair ◽  
Vocal Fold

scholarly journals A Regulatory miRNA–mRNA Network Is Associated with Tissue Repair Induced by Mesenchymal Stromal Cells in Acute Kidney Injury

2017 ◽  
Vol 7 ◽  
Author(s):  
Danilo Candido de Almeida ◽  
Ênio Jose Bassi ◽  
Hatylas Azevedo ◽  
Letícia Anderson ◽  
Clarice Silvia Taemi Origassa ◽  
...  
Keyword(s):  
Acute Kidney Injury ◽  
Mesenchymal Stromal Cells ◽  
Stromal Cells ◽  
Tissue Repair ◽  
Kidney Injury

scholarly journals Enhancing the Migration Ability of Mesenchymal Stromal Cells by Targeting the SDF-1/CXCR4 Axis

2013 ◽  
Vol 2013 ◽  
pp. 1-15 ◽  
Author(s):  
Leah A. Marquez-Curtis ◽  
Anna Janowska-Wieczorek
Keyword(s):  
Mesenchymal Stromal Cells ◽  
Stromal Cells ◽  
Tissue Repair ◽  
Inflammatory Responses ◽  
Surface Expression ◽  
Trophic Factors ◽  
Migration Ability ◽  
Therapeutic Benefits

Mesenchymal stromal cells (MSCs) are currently being investigated in numerous clinical trials of tissue repair and various immunological disorders based on their ability to secrete trophic factors and to modulate inflammatory responses. MSCs have been shown to migrate to sites of injury and inflammation in response to soluble mediators including the chemokine stromal cell-derived factor-(SDF-)1, but during in vitro culture expansion MSCs lose surface expression of key homing receptors particularly of the SDF-1 receptor, CXCR4. Here we review studies on enhancement of SDF-1-directed migration of MSCs with the premise that their improved recruitment could translate to therapeutic benefits. We describe our studies on approaches to increase the CXCR4 expression in in vitro-expanded cord blood-derived MSCs, namely, transfection, using the commercial liposomal reagent IBAfect, chemical treatment with the histone deacetylase inhibitor valproic acid, and exposure to recombinant complement component C1q. These methodologies will be presented in the context of other cell targeting and delivery strategies that exploit pathways involved in MSC migration. Taken together, these findings indicate that MSCs can be manipulated in vitro to enhance their in vivo recruitment and efficacy for tissue repair.

Hyaluronan Hydrogels for the Local Delivery of Mesenchymal Stromal Cells to the Injured Vocal Fold

2019 ◽  
Vol 28 (17) ◽  
pp. 1177-1190 ◽  
Author(s):  
Stellan Hertegård ◽  
Srinivasa Rao Nagubothu ◽  
Emma Malmström ◽  
Cecilia E. Ström ◽  
Anna Tolf ◽  
...  
Keyword(s):  
Mesenchymal Stromal Cells ◽  
Stromal Cells ◽  
Vocal Fold ◽  
Local Delivery

Abstract 484: Persistent activation of mesenchymal stromal cells (MSCs) and tissue repair in the etiology of desmoid tumors (DT)

2011 ◽  
Author(s):  
Adelaide M. Carothers ◽  
Hira Rizvi ◽  
Yvonne Heit ◽  
Rian M. Hasson ◽  
Jennifer Davids ◽  
...  
Keyword(s):  
Mesenchymal Stromal Cells ◽  
Stromal Cells ◽  
Tissue Repair ◽  
Desmoid Tumors

Mesenchymal stromal cells isolated from human fetal liver release soluble factors with a potential role in liver tissue repair

2019 ◽  
Vol 105 ◽  
pp. 14-26 ◽  
Author(s):  
Cinzia Maria Chinnici ◽  
Giada Pietrosi ◽  
Gioacchin Iannolo ◽  
Giandomenico Amico ◽  
Nicola Cuscino ◽  
...  
Keyword(s):  
Mesenchymal Stromal Cells ◽  
Stromal Cells ◽  
Liver Tissue ◽  
Tissue Repair ◽  
Potential Role ◽  
Fetal Liver ◽  
Soluble Factors ◽  
Human Fetal Liver

scholarly journals Isolation, expansion and differentiation of mesenchymal stromal cells from rabbits' bone marrow

2016 ◽  
Vol 36 (5) ◽  
pp. 423-430 ◽  
Author(s):  
Renato B. Eleotério ◽  
Rodrigo V. Sepúlveda ◽  
Emily C.C. Reis ◽  
Fabrício L. Valente ◽  
Andréa P.B. Borges
Keyword(s):  
Bone Marrow ◽  
Mesenchymal Stromal Cells ◽  
Stromal Cells ◽  
Tissue Repair ◽  
Mononuclear Cells ◽  
Culture Media ◽  
Rabbit Model ◽  
Proliferation Rate ◽  
Media Formulation

Abstract: Tissue engineering has been a fundamental technique in the regenerative medicine field, once it permits to build tri-dimensional tissue constructs associating undifferentiated mesenchymal cells (or mesenchymal stromal cells - MSCs) and scaffolds in vitro. Therefore, many studies have been carried out using these cells from different animal species, and rabbits are often used as animal model for in vivo tissue repair studies. However, most of the information available about MSCs harvesting and characterization is about human and murine cells, which brings some doubts to researchers who desire to work with a rabbit model in tissue repair studies based on MSCs. In this context, this study aimed to add and improve the information available in the scientific literature providing a complete technique for isolation, expansion and differentiation of MSCs from rabbits. Bone marrow mononuclear cells (BMMCs) from humerus and femur of rabbits were obtained and to evaluate their proliferation rate, three different culture media were tested, here referred as DMEM-P, DMEM´S and α-MEM. The BMMCs were also cultured in osteogenic, chondrogenic and adipogenic induction media to prove their multipotentiality. It was concluded that the techniques suggested in this study can provide a guideline to harvest and isolate MSCs from bone marrow of rabbits in enough amount to allow their expansion and, based on the laboratory experience where the study was developed, it is also suggested a culture media formulation to provide a better cell proliferation rate with multipotentiality preservation.

scholarly journals The Response of Vocal Fold Fibroblasts and Mesenchymal Stromal Cells to Vibration

PLoS ONE ◽  
2012 ◽  
Vol 7 (2) ◽  
pp. e30965 ◽  
Author(s):  
Joel Gaston ◽  
Beatriz Quinchia Rios ◽  
Rebecca Bartlett ◽  
Craig Berchtold ◽  
Susan L. Thibeault
Keyword(s):  
Mesenchymal Stromal Cells ◽  
Stromal Cells ◽  
Vocal Fold
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