scholarly journals Preparative Purification of Total Flavonoids from Sophora tonkinensis Gagnep. by Macroporous Resin Column Chromatography and Comparative Analysis of Flavonoid Profiles by HPLC-PAD

Molecules ◽  
2019 ◽  
Vol 24 (17) ◽  
pp. 3200 ◽  
Author(s):  
Mengyang Hou ◽  
Wenzhong Hu ◽  
Zhilong Xiu ◽  
Aili Jiang ◽  
Lei Men ◽  
...  
Keyword(s):  
Comparative Analysis ◽  
Column Chromatography ◽  
High Performance ◽  
Total Flavonoids ◽  
Macroporous Resin ◽  
Technological Parameters ◽  
Resin Column ◽  
Photodiode Array Detection ◽  
Before And After ◽  
Sophora Tonkinensis

For the full development and utilization of Sophora tonkinensis Gagnep., this study was primarily intended to established a simple and efficient approach for the preparative purification of total flavonoids from S. tonkinensis by macroporous resin column chromatography (MRCC). The adsorption and desorption characteristics of the total flavonoids on ten macroporous resins were first studied, and AB-8 resin was chosen as the most suitable, and the adsorption data were best fitted to the pseudo-second-order kinetics model and Langmuir isotherm model. Furthermore, the technological parameters for the purification of the total flavonoids were optimized using column chromatography. After a sample one-step purification procedure, the content of the total flavonoids increased by about 4.76-fold from 12.14% to 57.82%, with a recovery yield of 84.93%. In addition, the comparative analysis of the flavonoid extracts before and after purification was performed by high-performance liquid chromatography coupled with photodiode-array detection (HPLC-PAD). The results showed that the contents of six major flavonoids in the purified product were all higher than before the purification. Therefore, the AB-8 MRCC established in this work was a promising method for the industrial-scale purification of the total flavonoids from S. tonkinensis.

scholarly journals Isolation and characterization of ardicrenin from Ardisia crenata Sims

2009 ◽  
Vol 55 (No. 7) ◽  
pp. 305-310 ◽  
Author(s):  
Ma Yuan ◽  
Pu Shangrao ◽  
Cheng Qingsu ◽  
Ma Mingdong
Keyword(s):  
High Performance ◽  
Amorphous Powder ◽  
Gel Chromatography ◽  
Macroporous Resin ◽  
Resin Column ◽  
Isolation And Characterization ◽  
Natural Reserve ◽  
Silica Gel Chromatography ◽  
Counter Current

A new, effective and economical method to extract ardicrenin from <I>Ardisia crenata</I> Sims collected in the Wolong natural reserve, Sichuan, China, is established. <I>Ardisia crenata</I> Sims powder is counter-current extracted with 80% methanol reflux, decompressively enriched and centrifuged to defat. Supernatant is applied to macroporous resin column (AB-8) with 80% methanol, ardicrenin is isolated by silica gel chromatography with dichlormethane-acetoacetate-methanol (4:1.5:1) washing, and recrystallized in methanol. The final product which proved to be ardicrenin by analytic procedure including Furier transform infrared (FTIR) and ultraviolet spectrum (UV), mass spectroscopy (MS), nuclear magnetic resonance (NMR) and high performance liquid chromatography (HPLC) is white amorphous powder with yield of 1.59 ± 0.02%.

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