Separation and determination of the enantiomers of lactic acid and 2-hydroxyglutaric acid by chiral derivatization combined with gas chromatography and mass spectrometry

2018 ◽  
Vol 41 (12) ◽  
pp. 2576-2584 ◽  
Author(s):  
Xuemei Ding ◽  
Shuhai Lin ◽  
Hongbo Weng ◽  
Jianying Liang
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Lactic Acid ◽  
Chiral Derivatization ◽  
Hydroxyglutaric Acid

scholarly journals Exometabolites of some anaerobic microorganisms of human microflora

2011 ◽  
Vol 57 (1) ◽  
pp. 95-105 ◽  
Author(s):  
N.V. Beloborodova ◽  
I.T. Bairamov ◽  
A.Yu. Olenin ◽  
N.I. Fedotcheva
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Lactic Acid ◽  
Gas Chromatography Mass Spectrometry ◽  
Hydroxybutyric Acid ◽  
Anaerobic Microorganisms ◽  
Chromatography Mass Spectrometry ◽  
Hydroxyglutaric Acid ◽  
Anaerobic Microflora

Some exometabolites produced by basic representatives of human anaerobic microflora were investigated, detected by gas chromatography - mass spectrometry (GC-MS). In vitro besides lactic acid Bifidobacterium and Lactobacillus generate substantial amounts of phenyllactic and p-hydroxyphenyllactic acids. Clostridium produced 2-hydroxybutyric acid and to a lesser extent lactic and phenyllactic acids. In contrast to С. perfringens, C. sporogenes generates substantial amount of phenylpropionic and p-hydroxyphenylpropionic acids and less p-hydroxyphenyllactic acid. С. perfringens produced minor amounts of 2-hydroxyglutaric acid. Bacteroids are potent producers of succinic and fumaric acids; they also contribute to production of significant portion of lactic acid. E. lentum generate lactic, phenyllactic and succinic acids and form a characteristic only for ones (from studied microorganisms) 2-hydroxyhexanic and 2-hydroxy-3-methylbutyric acids.

Sign in / Sign up

Export Citation Format

Share Document