scholarly journals Analysis of the disaccharides derived from hyaluronic acid and chondroitin sulfate by capillary electrophoresis with sample stacking

2005 ◽  
Vol 28 (17) ◽  
pp. 2381-2389 ◽  
Author(s):  
Yifang Yang ◽  
Michael C. Breadmore ◽  
Wolfgang Thormann
Keyword(s):  
Capillary Electrophoresis ◽  
Hyaluronic Acid ◽  
Chondroitin Sulfate ◽  
Sample Stacking

Online preconcentration and determination of chondroitin sulfate, dermatan sulfate and hyaluronic acid in biological and cosmetic samples using capillary electrophoresis

2019 ◽  
Vol 42 (17) ◽  
pp. 2867-2874 ◽  
Author(s):  
Kanokporn Chindaphan ◽  
Kanet Wongravee ◽  
Thumnoon Nhujak ◽  
Thasinas Dissayabutra ◽  
Monpichar Srisa‐Art
Keyword(s):  
Capillary Electrophoresis ◽  
Hyaluronic Acid ◽  
Chondroitin Sulfate ◽  
Dermatan Sulfate ◽  
Online Preconcentration

scholarly journals Offline Selective Extraction Combined with Online Enrichment for Sensitive Analysis of Chondroitin Sulfate by Capillary Electrophoresis

2020 ◽  
Vol 58 (9) ◽  
pp. 868-874
Author(s):  
Jie Gong ◽  
Guanglian Zhou ◽  
Yuanhong Wu ◽  
Siying Zhang ◽  
Xiumei Liu
Keyword(s):  
Capillary Electrophoresis ◽  
Chondroitin Sulfate ◽  
Limit Of Detection ◽  
Background Electrolyte ◽  
Selective Extraction ◽  
Detection Sensitivity ◽  
Calibration Plot ◽  
Sample Stacking ◽  
Relative Standard ◽  
Experimental Parameters

Abstract A capillary electrophoresis (CE) method combined with online and offline enrichment for improving the detection sensitivity of chondroitin sulfate (CS) is established. The online enrichment method is based on the field-amplified sample stacking and large volume electrokinetic injection, and offline enrichment is based on the association between cetyltrimethylammonium chloride and CS. Experimental parameters affecting CE method such as the type and pH of background electrolyte, the injection mode and time and the steps of offline enrichment were optimized. Under optimum conditions, the calibration plot between CS concentration and peak area was linear in the range of 1 ~ 100 μg/mL. The enrichment factor was 130 times and the limit of detection was 50 ng/mL. The average recovery was 103.5% and the relative standard deviation of peak area was <2.0%. The method was successfully applied to the quantitative analysis of CS in drugs.

Glycosaminoglycans from growing antlers of wapiti (Cervus elaphus)

1997 ◽  
Vol 77 (4) ◽  
pp. 715-721 ◽  
Author(s):  
H. H. Sunwoo ◽  
L. Y. M. Sim ◽  
T. Nakano ◽  
R. J. Hudson ◽  
J. S. Sim
Keyword(s):  
Hyaluronic Acid ◽  
Chondroitin Sulfate ◽  
Cervus Elaphus ◽  
Molecular Size ◽  
Enzymatic Digestion ◽  
Gel Chromatography ◽  
Cellulose Acetate Electrophoresis ◽  
Cartilaginous Tissues ◽  
Chondroitin Sulfates ◽  
Velvet Antler

The emerging wapiti industry in North America is based largely on markets for velvet antlers which are used in oriental medicine. Despite the economic opportunity, enthusiasm has been dampened by incomplete understanding of the chemical and pharmacological properties of velvet antler. This study characterizes polysaccharide constituents of glycosaminoglycans in growing antler of wapiti (Cervus elaphus). Glycosaminoglycans were isolated from four sections (tip, upper, middle and base) of growing antlers, and were studied using cellulose acetate electrophoresis, gel electrophoresis, enzymatic digestion and gel chromatography. The tip and upper sections of the antler which are rich in cartilaginous tissues contained chondroitin sulfate as a major glycosaminoglycan with small amounts of hyaluronic acid. In the middle and base sections containing bone and bone marrow, chondroitin sulfate was also a major glycosaminoglycan with small amounts of hyaluronic acid and chondroitinase-ACI resistant materials. More than half of chondroitin sulfate from the middle and base sections had larger molecular size than did the chondroitin sulfates from the tip and upper sections. Key words: Glycosaminoglycans, chondroitin sulfate, antler, wapiti

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