Optimisation of gradient HPLC analysis of phenolic compounds and flavonoids in beer using a CoulArray detector

2004 ◽  
Vol 27 (15-16) ◽  
pp. 1345-1359 ◽  
Author(s):  
Lucie Řehová ◽  
Veronika Škeříková ◽  
Pavel Jandera
Keyword(s):  
Phenolic Compounds ◽  
Hplc Analysis ◽  
Gradient Hplc

Oxidative Stability of Edible Vegetable Oils Enriched in Polyphenols with Olive Leaf Extract

2007 ◽  
Vol 13 (6) ◽  
pp. 413-421 ◽  
Author(s):  
F.N. Salta ◽  
A. Mylona ◽  
A. Chiou ◽  
G. Boskou ◽  
N.K. Andrikopoulos
Keyword(s):  
Phenolic Compounds ◽  
Oxidative Stability ◽  
Antioxidant Capacity ◽  
Hplc Analysis ◽  
Leaf Extract ◽  
Olive Leaf ◽  
Total Polyphenols ◽  
Olive Leaf Extract ◽  
Rancimat Method ◽  
Oil Sunflower

Commercially available oils (olive oil, sunflower oil, palm oil, and a vegetable shortening) were enriched in polyphenols, by adding olive leaf extract. Addition of the extract was performed in such way that the oils were enriched with 200 mg/kg polyphenols. Total polyphenols of both enriched and commercial oils were estimated by the Folin—Ciocalteau assay, while identification and quantification of individual simple phenolic compounds was performed by GC/MS and of oleuropein by HPLC analysis. The enrichment resulted in the supplementation of the commercial oils mainly with oleuropein, hydroxytyrosol, and quercetin. Antioxidant capacity and oxidative stability of the enriched oils and the commercial ones were assessed by the 1,1-diphenyl-2-picrylhydrazyl radical (DPPH•) scavenging assay and the Rancimat method, respectively. Both antioxidant capacity and oxidative stability were substantially improved for all the oils studied after supplementation. By the procedure adopted, oils rich in polyphenols, mainly in oleuropein, can be produced with olive leaf extract supplementation.

HPLC Analysis of Phenolic Compounds in Yellow Sweet-Clover

2004 ◽  
Vol 38 (4) ◽  
pp. 195-196 ◽  
Author(s):  
V. N. Bubenchikova ◽  
I. L. Drozdova
Keyword(s):  
Phenolic Compounds ◽  
Hplc Analysis ◽  
Sweet Clover

scholarly journals Biotransformation of Monocyclic Phenolic Compounds by Bacillus licheniformis TAB7

2019 ◽  
Vol 8 (1) ◽  
pp. 26 ◽  
Author(s):  
Enock Mpofu ◽  
Joydeep Chakraborty ◽  
Chiho Suzuki-Minakuchi ◽  
Kazunori Okada ◽  
Toshiaki Kimura ◽  
...  
Keyword(s):  
Phenolic Compounds ◽  
Bacillus Licheniformis ◽  
Genome Analysis ◽  
Industrial Waste ◽  
Plant Material ◽  
Hplc Analysis ◽  
Degradation Pathway ◽  
Acid Decarboxylase ◽  
Material Decomposition ◽  
Phenolic Acid Decarboxylase

Bacillus licheniformis strain TAB7 is a bacterium used as a commercial deodorizing agent for compost in Japan. In this work, its ability to biotransform the following monocyclic phenolic compounds was assessed: ferulate, vanillate, p-coumarate, caffeate, protocatechuate, syringate, vanillin, and cinnamate (a precursor for some phenolic compounds). These compounds are abundant in composting material and are reported to have allelopathic properties. They come from sources such as plant material decomposition or agro-industrial waste. Biotransformation assays were carried out in LB supplemented with 0.2 mg/mL of an individual phenolic compound and incubated for up to 15 days followed by extraction and HPLC analysis. The results showed that TAB7 could biotransform ferulate, caffeate, p-coumarate, vanillate, protocatechuate, and vanillin. It, however, had a poor ability to transform cinnamate and syringate. LC-MS/MS analysis showed that ferulate was transformed into 4-vinylguaiacol as the final product, while caffeate was transformed into 4-ethylcatechol. TAB7 genome analysis suggested that, while TAB7 may not mineralize phenolic compounds, it harbored genes possibly encoding phenolic acid decarboxylase, vanillate decarboxylase, and some protocatechuate degradation pathway enzymes, which are involved in the catabolism of phenolic compounds known to have negative allelopathy on some plants. The results thus suggested that TAB7 can reduce such phenolic compounds in compost.

scholarly journals Profiling of the Major Phenolic Compounds and Their Biosynthesis Genes in Sophora flavescens Aiton

2018 ◽  
Vol 2018 ◽  
pp. 1-9 ◽  
Author(s):  
Jeongyeo Lee ◽  
Jaeeun Jung ◽  
Seung-Hyun Son ◽  
Hyun-Bi Kim ◽  
Young-Hee Noh ◽  
...  
Keyword(s):  
Phenolic Compounds ◽  
Phenolic Acid ◽  
Hplc Analysis ◽  
Developmental Stages ◽  
Biological Activities ◽  
Expression Patterns ◽  
Coumaric Acid ◽  
Sophora Flavescens ◽  
Catechin Hydrate ◽  
Aerial Parts

Sophorae Radix (Sophora flavescens Aiton) has long been used in traditional medicine in East Asia due to the various biological activities of its secondary metabolites. Endogenous contents of phenolic compounds (phenolic acid, flavonol, and isoflavone) and the main bioactive compounds of Sophorae Radix were analyzed based on the qualitative HPLC analysis and evaluated in different organs and at different developmental stages. In total, 11 compounds were detected, and the composition of the roots and aerial parts (leaves, stems, and flowers) was significantly different. trans-Cinnamic acid and p-coumaric acid were observed only in the aerial parts. Large amounts of rutin and maackiain were detected in the roots. Four phenolic acid compounds (benzoic acid, caffeic acid, ferulic acid, and chlorogenic acid) and four flavonol compounds (kaempferol, catechin hydrate, epicatechin, and rutin) were higher in aerial parts than in roots. To identify putative genes involved in phenolic compounds biosynthesis, a total of 41 transcripts were investigated. Expression patterns of these selected genes, as well as the multiple isoforms for the genes, varied by organ and developmental stage, implying that they are involved in the biosynthesis of various phenolic compounds both spatially and temporally.

scholarly journals HPLC Analysis of Chemical Composition of Selected Jordanian Medicinal Plants and Their Bioactive Properties

2018 ◽  
Vol 34 (5) ◽  
pp. 2397-2403
Author(s):  
Fuad Al-Rimawi ◽  
Fadi Alakhras ◽  
Wael A. Al-Zereini ◽  
Hammad K. Aldal'in ◽  
Saleh Abu-Lafi ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Chemical Composition ◽  
Phenolic Compounds ◽  
Medicinal Plants ◽  
Plant Extracts ◽  
Hplc Analysis ◽  
Room Temperature ◽  
Bacterial Strains ◽  
E Coli ◽  
Bioactive Properties

Three medicinal plants grown wild in Jordan, namely Achillea santolina L, Achillea fragrantisimma, Asteriscus graveolens (Forssk) Less, were extracted with ethyl acetate by continuous shaking at room temperature for three days. The antibacterial activity of the crude extract was evaluated. The extracts were analyzed for their phenolic and flavonoids content by HPLC-PDA. The HPLC analysis of the plant extracts revealed the presence of flavonoids and phenolic compounds in the three plant extracts. Results revealed a strong antibacterial activity of A. graveolens against three bacterial strains (B. subtilis, E. coli, and S.aureus) while A. fragrantissima inhibited the growth of B. subtilis. Bioactivities were attributed mainly to the immense content of phenol-based compounds in plants.

RP-HPLC analysis of phenolic compounds and flavonoids in beverages and plant extracts using a CoulArray detector

2005 ◽  
Vol 28 (9-10) ◽  
pp. 1005-1022 ◽  
Author(s):  
Pavel Jandera ◽  
Veronika Škeříková ◽  
Lucie Řehová ◽  
Tomáš Hájek ◽  
Lucie Baldriánová ◽  
...  
Keyword(s):  
Phenolic Compounds ◽  
Plant Extracts ◽  
Hplc Analysis ◽  
Rp Hplc
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