The composition of cell walls from grape skin in Vitis vinifera intraspecific hybrids

2017 ◽  
Vol 97 (12) ◽  
pp. 4029-4035 ◽  
Author(s):  
Rafael Apolinar-Valiente ◽  
Encarna Gómez-Plaza ◽  
Nancy Terrier ◽  
Thierry Doco ◽  
José María Ros-García
Keyword(s):  
Vitis Vinifera ◽  
Cell Walls ◽  
Grape Skin ◽  
Intraspecific Hybrids

Isolation and characterization of cell walls from the mesocarp of mature grape berries (Vitis vinifera)

2020 ◽  
Author(s):  
KJ Nunan ◽  
Ian Sims ◽  
A Bacic ◽  
SP Robinson ◽  
GB Fincher
Keyword(s):  
Vitis Vinifera ◽  
Cell Walls ◽  
Vascular Tissue ◽  
Compositional Analysis ◽  
Water Soluble ◽  
Vitis Vinifera L ◽  
Nylon Mesh ◽  
Cytoplasmic Material ◽  
Isolation And Characterization ◽  
Tissue Homogenates

Cell walls have been isolated from the mesocarp of mature grape (Vitis vinifera L.) berries. Tissue homogenates were suspended in 80% (v/v) ethanol to minimise the loss of water-soluble wall components and wet-sieved on nylon mesh to remove cytoplasmic material. The cell wall fragments retained on the sieve were subsequently treated with buffered phenol at pH 7.0, to inactivate any wall-bound enzymes and to dislodge small amounts of cytoplasmic proteins that adhered to the walls. Finally, the wall preparation was washed with chloroform/methanol (1:1, v/v) to remove lipids and dried by solvent exchange. Scanning electron microscopy showed that the wall preparation was essentially free of vascular tissue and adventitious protein of cytoplasmic origin. Compositional analysis showed that the walls consisted of approximately 90% by weight of polysaccharide and less than 10% protein. The protein component of the walls was shown to be rich in arginine and hydroxyproline residues. Cellulose and polygalacturonans were the major constituents, and each accounted for 30-40% by weight of the polysaccharide component of the walls. Substantial varietal differences were observed in the relative abundance of these two polysaccharides. Xyloglucans constituted approximately 10% of the polysaccharide fraction and the remainder was made up of smaller amounts of mannans, heteroxylans, arabinans and galactans.

Antihypertensive effects of the Vitis vinifera grape skin (ACH09) extract consumption elicited by functional improvement of P1 (A1) and P2 (P2X1) purinergic receptors in diabetic and hypertensive rats

2019 ◽  
Vol 8 ◽  
pp. 100146 ◽  
Author(s):  
Guilherme Henrique Souza Bomfim ◽  
Diego Castro Musial ◽  
Regiane Miranda-Ferreira ◽  
Sthefani Rodrigues Nascimento ◽  
Aron Jurkiewicz ◽  
...  
Keyword(s):  
Vitis Vinifera ◽  
Purinergic Receptors ◽  
Functional Improvement ◽  
Hypertensive Rats ◽  
Grape Skin

Culture of Protoplasts From Grape Vine Pericarp Callus

1974 ◽  
Vol 1 (3) ◽  
pp. 371 ◽  
Author(s):  
KGM Skene
Keyword(s):  
Vitis Vinifera ◽  
Cell Walls ◽  
Callus Cultures ◽  
Lower Proportion ◽  
Grape Vine ◽  
Solid Media ◽  
Semi Solid

Protoplasts isolated from callus cultures of the pericarp of Vitis vinifera cv. Sultana regenerated cell walls within 3 days, when cultured in semi-solid media adjusted to 0.4-0.5 osmolal. Up to 12% of the cells divided during the next 7 days. A lower proportion passed through two division cycles.

Following the Compositional Changes of Fresh Grape Skin Cell Walls during the Fermentation Process in the Presence and Absence of Maceration Enzymes

2015 ◽  
Vol 63 (10) ◽  
pp. 2798-2810 ◽  
Author(s):  
Anscha J. J. Zietsman ◽  
John P. Moore ◽  
Jonatan U. Fangel ◽  
William G. T. Willats ◽  
Johan Trygg ◽  
...  
Keyword(s):  
Cell Walls ◽  
Fermentation Process ◽  
Grape Skin ◽  
Skin Cell ◽  
Compositional Changes ◽  
Presence And Absence

Differences in morphology and composition of skin and pulp cell walls from grapes (Vitis vinifera L.): technological implications

2007 ◽  
Vol 227 (1) ◽  
pp. 223-231 ◽  
Author(s):  
Ana Ortega-Regules ◽  
José María Ros-García ◽  
Ana Belén Bautista-Ortín ◽  
José María López-Roca ◽  
Encarna Gómez-Plaza
Keyword(s):  
Vitis Vinifera ◽  
Cell Walls ◽  
Vitis Vinifera L ◽  
Pulp Cell
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