Culture of Protoplasts From Grape Vine Pericarp Callus

1974 ◽  
Vol 1 (3) ◽  
pp. 371 ◽  
Author(s):  
KGM Skene
Keyword(s):  
Vitis Vinifera ◽  
Cell Walls ◽  
Callus Cultures ◽  
Lower Proportion ◽  
Grape Vine ◽  
Solid Media ◽  
Semi Solid

Protoplasts isolated from callus cultures of the pericarp of Vitis vinifera cv. Sultana regenerated cell walls within 3 days, when cultured in semi-solid media adjusted to 0.4-0.5 osmolal. Up to 12% of the cells divided during the next 7 days. A lower proportion passed through two division cycles.

Isolation and characterization of cell walls from the mesocarp of mature grape berries (Vitis vinifera)

2020 ◽  
Author(s):  
KJ Nunan ◽  
Ian Sims ◽  
A Bacic ◽  
SP Robinson ◽  
GB Fincher
Keyword(s):  
Vitis Vinifera ◽  
Cell Walls ◽  
Vascular Tissue ◽  
Compositional Analysis ◽  
Water Soluble ◽  
Vitis Vinifera L ◽  
Nylon Mesh ◽  
Cytoplasmic Material ◽  
Isolation And Characterization ◽  
Tissue Homogenates

Cell walls have been isolated from the mesocarp of mature grape (Vitis vinifera L.) berries. Tissue homogenates were suspended in 80% (v/v) ethanol to minimise the loss of water-soluble wall components and wet-sieved on nylon mesh to remove cytoplasmic material. The cell wall fragments retained on the sieve were subsequently treated with buffered phenol at pH 7.0, to inactivate any wall-bound enzymes and to dislodge small amounts of cytoplasmic proteins that adhered to the walls. Finally, the wall preparation was washed with chloroform/methanol (1:1, v/v) to remove lipids and dried by solvent exchange. Scanning electron microscopy showed that the wall preparation was essentially free of vascular tissue and adventitious protein of cytoplasmic origin. Compositional analysis showed that the walls consisted of approximately 90% by weight of polysaccharide and less than 10% protein. The protein component of the walls was shown to be rich in arginine and hydroxyproline residues. Cellulose and polygalacturonans were the major constituents, and each accounted for 30-40% by weight of the polysaccharide component of the walls. Substantial varietal differences were observed in the relative abundance of these two polysaccharides. Xyloglucans constituted approximately 10% of the polysaccharide fraction and the remainder was made up of smaller amounts of mannans, heteroxylans, arabinans and galactans.

Natamycin release from alginate active films to liquid and semi-solid media

2021 ◽  
Author(s):  
Andréa C. K. Bierhalz ◽  
Mariana A. da Silva ◽  
Theo G. Kieckbusch
Keyword(s):  
Solid Media ◽  
Semi Solid

scholarly journals Occurrence of diazotrophic bacteria in Araucaria angustifolia

2007 ◽  
Vol 64 (3) ◽  
pp. 303-304 ◽  
Author(s):  
Rafaela de Fátima Neroni ◽  
Elke Jurandy Bran Nogueira Cardoso
Keyword(s):  
16S Rrna ◽  
Acetylene Reduction ◽  
Araucaria Angustifolia ◽  
Diazotrophic Bacteria ◽  
Araucaria Forest ◽  
Solid Media ◽  
Reduction Assay ◽  
Semi Solid ◽  
Rrna Sequences ◽  
16S Rrna Sequences

Araucaria angustifolia is an environmentally threatened tree and the whole biota of the Araucaria Forest should be investigated with the aim of its preservation. Diazotrophic bacteria are extremely important for the maintenance of ecosystems, but they have never been studied in Araucaria Forests. In this study, diazotrophic bacteria were isolated from Araucaria roots and soil, when grown in semi-specific, semi-solid media. The diazotrophic character of some recovered isolates could be confirmed using the acetylene reduction assay. According to their 16S rRNA sequences, most of these isolates belong to the genus Burkholderia.

Genetic transformation of Stella De Oro daylily by particle bombardment

2003 ◽  
Vol 83 (4) ◽  
pp. 873-876 ◽  
Author(s):  
A. N. Aziz ◽  
R. J. Sauvé ◽  
S. Zhou
Keyword(s):  
Southern Blotting ◽  
Basal Medium ◽  
Callus Cultures ◽  
Naphthaleneacetic Acid ◽  
Ms Medium ◽  
Chain Reaction ◽  
Polymerase Chain ◽  
Semi Solid ◽  
Independent Transformation

Daylily (Hemerocallis sp. ‘Stella de Oro’) callus cultures initiated from ovules were bombarded with gold particles coated with plasmid harboring Basta® resistance gene. Resulting putative transgenic calli were selected after 3 wk on semi-solid Murashige and Skoog’s (MS) basal medium supplemented with 10 mg L-1 1-naphthaleneacetic acid, 2 mg L-1 6-benzylaminopurine and 3 mg L-1 phosphinothricin (PPT). Surviving calli regenerated shoots after 2 mo on semi-solid MS medium supplemented with 2 mg L-1 thiadiazuron and 1 mg L-1 PPT. Polymerase chain reaction and Southern blotting were used to confirm independent transformation events. Key words: Basta® resistance, in vitro, Hemerocallis

scholarly journals Esterase isozymes patterns of grape vine (Vitis vinifera L.) are altered in response to fungicide exposure

2015 ◽  
Vol 37 (4) ◽  
pp. 463 ◽  
Author(s):  
Gleice Ribeiro Orasmo ◽  
Sandra Aparecida Oliveira-Collet ◽  
Claudete Aparecida Mangolin ◽  
Ana Silvia Lapenta ◽  
Maria de Fátima Pires da Silva Machado
Keyword(s):  
Vitis Vinifera ◽  
Esterase Isozymes ◽  
Grape Vine

scholarly journals Density and diversity of diazotrophic bacteria isolated from Amazonian soils using N-free semi-solid media

2011 ◽  
Vol 68 (5) ◽  
pp. 518-525 ◽  
Author(s):  
Krisle da Silva ◽  
Rafaela Simão Abrahão Nóbrega ◽  
Adriana Silva Lima ◽  
Alexandre Barberi ◽  
Fatima Maria de Souza Moreira
Keyword(s):  
Diazotrophic Bacteria ◽  
Solid Media ◽  
Semi Solid

T lymphoma variants with specifically altered growth in semi-solid media

1983 ◽  
Vol 32 (5) ◽  
pp. 641-643
Author(s):  
Jean-Michel Claverie ◽  
Melvin Cohn
Keyword(s):  
Solid Media ◽  
T Lymphoma ◽  
Semi Solid
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