Development and histochemistry of the cells, cell walls, and cuticle of the dermal system of fruit of the grape,Vitis vinifera L.

PROTOPLASMA ◽  
1979 ◽  
Vol 99 (4) ◽  
pp. 347-365 ◽  
Author(s):  
J. A. Considine ◽  
R. B. Knox
Keyword(s):  
Vitis Vinifera ◽  
Cell Walls ◽  
Vitis Vinifera L

Isolation and characterization of cell walls from the mesocarp of mature grape berries (Vitis vinifera)

2020 ◽  
Author(s):  
KJ Nunan ◽  
Ian Sims ◽  
A Bacic ◽  
SP Robinson ◽  
GB Fincher
Keyword(s):  
Vitis Vinifera ◽  
Cell Walls ◽  
Vascular Tissue ◽  
Compositional Analysis ◽  
Water Soluble ◽  
Vitis Vinifera L ◽  
Nylon Mesh ◽  
Cytoplasmic Material ◽  
Isolation And Characterization ◽  
Tissue Homogenates

Cell walls have been isolated from the mesocarp of mature grape (Vitis vinifera L.) berries. Tissue homogenates were suspended in 80% (v/v) ethanol to minimise the loss of water-soluble wall components and wet-sieved on nylon mesh to remove cytoplasmic material. The cell wall fragments retained on the sieve were subsequently treated with buffered phenol at pH 7.0, to inactivate any wall-bound enzymes and to dislodge small amounts of cytoplasmic proteins that adhered to the walls. Finally, the wall preparation was washed with chloroform/methanol (1:1, v/v) to remove lipids and dried by solvent exchange. Scanning electron microscopy showed that the wall preparation was essentially free of vascular tissue and adventitious protein of cytoplasmic origin. Compositional analysis showed that the walls consisted of approximately 90% by weight of polysaccharide and less than 10% protein. The protein component of the walls was shown to be rich in arginine and hydroxyproline residues. Cellulose and polygalacturonans were the major constituents, and each accounted for 30-40% by weight of the polysaccharide component of the walls. Substantial varietal differences were observed in the relative abundance of these two polysaccharides. Xyloglucans constituted approximately 10% of the polysaccharide fraction and the remainder was made up of smaller amounts of mannans, heteroxylans, arabinans and galactans.

Differences in morphology and composition of skin and pulp cell walls from grapes (Vitis vinifera L.): technological implications

2007 ◽  
Vol 227 (1) ◽  
pp. 223-231 ◽  
Author(s):  
Ana Ortega-Regules ◽  
José María Ros-García ◽  
Ana Belén Bautista-Ortín ◽  
José María López-Roca ◽  
Encarna Gómez-Plaza
Keyword(s):  
Vitis Vinifera ◽  
Cell Walls ◽  
Vitis Vinifera L ◽  
Pulp Cell

Preliminary histological observations on grapevine affected by esca disease

2011 ◽  
Vol 6 (2) ◽  
pp. 216-222
Author(s):  
Lucia Andreini ◽  
Raffaella Viti ◽  
Giancarlo Scalabrelli
Keyword(s):  
Early Detection ◽  
Vitis Vinifera ◽  
Crystal Violet ◽  
Cell Walls ◽  
Optical Microscope ◽  
Future Application ◽  
Vitis Vinifera L ◽  
Cabernet Sauvignon ◽  
Esca Disease ◽  
Histological Characteristics

AbstractEsca is a destructive disease of the woody tissues of grapevine (Vitis vinifera L.) and due to the complexity of disease many aspects of host-pathogen interactions are not clearly understood. The histological characteristics of esca symptomatic petioles and internodes, collected from Cabernet Sauvignon and Sangiovese grapevine were studied. The tissues were fixed in FAA, dehydrated and embedded in Histoplast. To identify the lignified cell walls the sections were stained by Crystal violet and Erythrosin B and observed using an optical microscope. The main feature of tissues infected by esca disease was the minor lignification of vascular tissues, which was observed in petiole tissues before appearance of esca symptoms. The opportunity to utilize the histological examination of tissues as a method for the early detection of esca infections is hypothesized for the future application.

Isolation and characterization of cell walls from the mesocarp of mature grape berries (Vitis vinifera)

2020 ◽  
Author(s):  
KJ Nunan ◽  
Ian Sims ◽  
A Bacic ◽  
SP Robinson ◽  
GB Fincher
Keyword(s):  
Vitis Vinifera ◽  
Cell Walls ◽  
Vascular Tissue ◽  
Compositional Analysis ◽  
Water Soluble ◽  
Vitis Vinifera L ◽  
Nylon Mesh ◽  
Cytoplasmic Material ◽  
Isolation And Characterization ◽  
Tissue Homogenates

Cell walls have been isolated from the mesocarp of mature grape (Vitis vinifera L.) berries. Tissue homogenates were suspended in 80% (v/v) ethanol to minimise the loss of water-soluble wall components and wet-sieved on nylon mesh to remove cytoplasmic material. The cell wall fragments retained on the sieve were subsequently treated with buffered phenol at pH 7.0, to inactivate any wall-bound enzymes and to dislodge small amounts of cytoplasmic proteins that adhered to the walls. Finally, the wall preparation was washed with chloroform/methanol (1:1, v/v) to remove lipids and dried by solvent exchange. Scanning electron microscopy showed that the wall preparation was essentially free of vascular tissue and adventitious protein of cytoplasmic origin. Compositional analysis showed that the walls consisted of approximately 90% by weight of polysaccharide and less than 10% protein. The protein component of the walls was shown to be rich in arginine and hydroxyproline residues. Cellulose and polygalacturonans were the major constituents, and each accounted for 30-40% by weight of the polysaccharide component of the walls. Substantial varietal differences were observed in the relative abundance of these two polysaccharides. Xyloglucans constituted approximately 10% of the polysaccharide fraction and the remainder was made up of smaller amounts of mannans, heteroxylans, arabinans and galactans.

Changements cytochimiques et ultrastructuraux des parois cellulaires de la pellicule du raisin, Vitis vinifera, durant la croissance et la maturation de la baie

1987 ◽  
Vol 65 (7) ◽  
pp. 1343-1355 ◽  
Author(s):  
Homa Rajaei
Keyword(s):  
Vitis Vinifera ◽  
Growth Phase ◽  
Cell Walls ◽  
Periodic Acid ◽  
Grape Berry ◽  
Pinot Noir ◽  
Vitis Vinifera L ◽  
Layer Deposition ◽  
Second Growth ◽  
Cuticle Thickness

Cytochemical and ultrastructural analysis of cell wall characteristics was performed in the dermal system of the grape berry (Vitis vinifera L. cv. Pinot noir), using a test for polysaccharides (periodic acid – thiosemicarbazide – silver proteinate, associated with dimethylsulfoxide extraction). Samples were examined at successive stages of fruit development. The main events of the first growth phase, i.e., thickening of hypodermal cell walls and their tangential extension, continued during the second growth phase. At all the stages of grape development, tangentially expanding walls were typical of actively growing plant cell walls, with an ordered texture, new layer deposition, and progressive rotation of microfibrillar subunits (bow-shaped patterns). At maturity, hydration, swelling of the walls, and an increase in the amount of soluble pectic substances were noticed. No redifferentiation of the cell walls occurred. The cuticle, already differentiated into two layers at the first growth phase, was clearly lamellar during the second. At maturity a number of layers, distinguishable by their cytochemical reactivity, were detected. Cuticle thickness increased during "véraison" and was maintained throughout the second growth phase. A considerable increase in the content of epicuticular waxes was noticed during ripening.

Resveratrol oligomers from Vitis Vinifera L. stems

Planta Medica ◽  
2007 ◽  
Vol 73 (09) ◽  
Author(s):  
H Amira-Guebailia ◽  
T Richard ◽  
S Rouaiguia ◽  
P Waffo Tueguo ◽  
JC Delaunay ◽  
...  
Keyword(s):  
Vitis Vinifera ◽  
Vitis Vinifera L

Biotransformation of 4-hydroxybenzoic acid in the rhizosphere of grapevine (Vitis vinifera L.)

2017 ◽  
Vol 40 (1) ◽  
pp. 95-102 ◽  
Author(s):  
B. Wang ◽  
T. Zhou1 ◽  
K. Li ◽  
X.W. Guo ◽  
Y.S. Guo ◽  
...  
Keyword(s):  
Vitis Vinifera ◽  
Vitis Vinifera L ◽  
Hydroxybenzoic Acid

Sistemas atmosféricos em Santa Catarina no período da maturação à colheita de videira Cabernet Sauvignon

Agrometeoros ◽  
2018 ◽  
Vol 25 (2) ◽  
Author(s):  
Cristina Pandolfo ◽  
Marilene De Lima ◽  
Angelo Mendes Massignam ◽  
Aparecido Lima da Silva ◽  
Luiz Albano Hammes
Keyword(s):  
Vitis Vinifera ◽  
Vitis Vinifera L ◽  
Cabernet Sauvignon ◽  
Santa Catarina

Os sistemas atmosféricos exercem um papel significativo no clima de uma região e poucos trabalhos determinaram os impactos dos sistemas atmosféricos no de­senvolvimento e na produção de videira. Os objetivos deste trabalho foram deter­minar a frequência de ocorrência dos sistemas atmosféricos durante o período da maturação à colheita da videira (Vitis vinifera L.) var. Cabernet Sauvignon para as duas regiões produtoras de Santa Catarina em diferentes safras e determinar a as­sociação entre a ocorrência dos sistemas atmosféricos e as regiões produtoras e as safras. Os sistemas atmosféricos foram identificados durante as safras de 2005/2006 à 2008/2009. Os municípios de Água Doce e Campos Novos foram escolhidos para re­presentar a região produtora 1 e os municípios de Bom Retiro e São Joaquim para re­presentar a região produtora 2. As frequências de ocorrências dos sistemas atmosfé­ricos durante o período da maturação à colheita de videira são muito semelhantes entre as regiões, somente houve diferença na frequência de ocorrência do sistema jato subtropical entre regiões. Houve uma diferença significativa da ocorrência da frequência dos sistemas atmosféricos entre safras.

Sign in / Sign up

Export Citation Format

Share Document