scholarly journals In vitro screening of natural feed additives from crustaceans, diatoms, seaweeds and plant extracts to manipulate rumen fermentation

2015 ◽  
Vol 96 (9) ◽  
pp. 3069-3078 ◽  
Author(s):  
Alejandro Belanche ◽  
Eva Ramos-Morales ◽  
C Jamie Newbold
Keyword(s):  
Plant Extracts ◽  
Rumen Fermentation ◽  
Feed Additives ◽  
In Vitro Screening

In vitro screening of selected feed additives, plant essential oils and plant extracts for rumen methane mitigation

2013 ◽  
Vol 94 (6) ◽  
pp. 1191-1196 ◽  
Author(s):  
Zoey Durmic ◽  
Peter J Moate ◽  
Richard Eckard ◽  
Dean K Revell ◽  
Richard Williams ◽  
...  
Keyword(s):  
Essential Oils ◽  
Plant Extracts ◽  
Feed Additives ◽  
In Vitro Screening ◽  
Methane Mitigation ◽  
Plant Essential Oils

scholarly journals Combination Effects of Plant Extracts Rich in Tannins and Saponins as Feed Additives for Mitigating in Vitro Ruminal Methane and Ammonia Formation

Animals ◽  
2020 ◽  
Vol 10 (9) ◽  
pp. 1531 ◽  
Author(s):  
Anuraga Jayanegara ◽  
Yogianto Yogianto ◽  
Elizabeth Wina ◽  
Asep Sudarman ◽  
Makoto Kondo ◽  
...  
Keyword(s):  
Plant Extracts ◽  
Ammonia Concentration ◽  
Methane Emissions ◽  
Feed Additives ◽  
Combination Effects ◽  
Pure Methanol ◽  
High Forage ◽  
Substrate Types ◽  
Ammonia Formation

The objective of this experiment was to test the effects of combining plant extracts rich in tannins and saponins at varying proportions on in vitro ruminal methane and ammonia formation. Tannins were extracted from Swietenia mahogani leaves and saponins from Sapindus rarak fruits with various solvents. The extracts obtained with the most efficient solvents (tannins: 75% water and 25% methanol; saponins: pure methanol) were then used in vitro. The treatments consisted of two substrate types (high-forage (HF) or high-concentrate (HC) diets) and five extract combinations (tannins: saponins, 1:0, 3:1, 1:1, 1:3, and 0:1) added at 2 mg/mL in incubation liquid. In vitro incubation was performed in four runs, with each treatment being represented with two replicates per run. The addition of plant extracts rich in tannins and saponins, either individually or in combination, decreased the methane proportion of total gas in both the HF (p < 0.05) and HC (p < 0.05) diets. The effects of the plant extracts rich in tannins and saponins were generally additive in mitigating methane emissions. Favorable associative effects between the extracts were observed in the ammonia concentration, both in the HF (p < 0.001) and HC (p < 0.01) diets and in the methane proportion of total gas, with a 1:3 mixture of tannins and saponins added to the HC diet (p < 0.05).

In vitro screening of Algerian steppe browse plants for digestibility, rumen fermentation profile and methane mitigation

2019 ◽  
Vol 94 (4) ◽  
pp. 1433-1443 ◽  
Author(s):  
Lyas Bouazza ◽  
Souhil Boufennara ◽  
Mustapha Bensaada ◽  
Azzeddine Zeraib ◽  
Khalid Rahal ◽  
...  
Keyword(s):  
Rumen Fermentation ◽  
In Vitro Screening ◽  
Methane Mitigation ◽  
Fermentation Profile

Validation of a colorimetric assay for the in vitro screening of inhibitors of angiotensin-converting enzyme (ACE) from plant extracts

Phytomedicine ◽  
2005 ◽  
Vol 12 (6-7) ◽  
pp. 424-432 ◽  
Author(s):  
C.P. Serra ◽  
S.F. Côrtes ◽  
J.A. Lombardi ◽  
A. Braga de Oliveira ◽  
F.C. Braga
Keyword(s):  
Angiotensin Converting Enzyme ◽  
Plant Extracts ◽  
Colorimetric Assay ◽  
In Vitro Screening ◽  
Converting Enzyme

The effect of essential oils of Zataria multiflora and Mentha spicata on the in vitro rumen fermentation, and growth and deaminative activity of amino acid-fermenting bacteria isolated from Mehraban sheep

2014 ◽  
Vol 54 (3) ◽  
pp. 299 ◽  
Author(s):  
M. Taghavi-Nezhad ◽  
D. Alipour ◽  
M. D. Flythe ◽  
P. Zamani ◽  
G. Khodakaramian
Keyword(s):  
Essential Oils ◽  
Rumen Fermentation ◽  
Gas Production ◽  
Feed Additives ◽  
Rrna Gene ◽  
Ammonia Production ◽  
Mentha Spicata ◽  
Zataria Multiflora ◽  
Rumen Microbes

Gas (CO2 and CH4) and ammonia production in the rumen represent major sources of lost carbon and nitrogen, respectively. The essential oils of some plants have been shown to decrease gas and ammonia production by selectively inhibiting rumen microbes. Particularly, those of Zataria multiflora (ZEO; thymol 21%, carvacrol 32%) and Mentha spicata (SEO; carvone 55%) were evaluated in vitro as ruminant-feed additives. The experiments employed mixed rumen microbes and a hyper-ammonia-producing bacterium (HAP) isolated from the rumen of a Mehraban sheep. Both ZEO and SEO decreased in vitro fibre digestibility and also gas production by mixed rumen microbes that were fermenting a typical growing-lamb diet. ZEO decreased ammonia concentration in mixed culture of rumen microbes, but SEO exerted the opposite effect. A bacterial isolate (MT8) was obtained from the rumen of a Mehraban sheep, and the 16S rRNA gene sequence indicated that it was most closely related to Clostridium bifermentans. Isolate MT8 exhibited rapid ammonia production when peptides were the growth substrate, which indicated that MT8 was a HAP. Both oils inhibited the growth and ammonia production of isolate MT8. However, ZEO decreased ammonia production at lower doses, and to a greater degree, than did SEO. These results indicated that both essential oils could potentially be used to modulate rumen fermentation. The detrimental effects on fibre digestion could be problematic in high-forage diets, and this requires further investigation. Isolate MT8 is the first described HAP from the Mehraban sheep rumen. Results on ammonia production by isolate MT8 and mixed rumen microbes indicate differential mode of action of each oil on this parameter.

In vitro screening of American plant extracts on Trypanosoma cruzi and Trichomonas vaginalis

2000 ◽  
Vol 71 (1-2) ◽  
pp. 101-107 ◽  
Author(s):  
S Muelas-Serrano ◽  
J.J Nogal ◽  
R.A Martı́nez-Dı́az ◽  
J.A Escario ◽  
A.R Martı́nez-Fernández ◽  
...  
Keyword(s):  
Trypanosoma Cruzi ◽  
Plant Extracts ◽  
Trichomonas Vaginalis ◽  
In Vitro Screening
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