Separation of polyphenols and arecoline from areca nut (Areca catechu L.) by solvent extraction, its antioxidant activity, and identification of polyphenols

2013 ◽  
Vol 93 (10) ◽  
pp. 2580-2589 ◽  
Author(s):  
Yogita V Chavan ◽  
Rekha S Singhal
Keyword(s):  
Antioxidant Activity ◽  
Solvent Extraction ◽  
Areca Nut ◽  
Areca Catechu

scholarly journals Antioxidant Activity of Areca Nut to Human Health: Effect on Oral Cancer Cell Lines and Immunomodulatory Activity

2021 ◽  
Author(s):  
Liza Meutia Sari
Keyword(s):  
Antioxidant Activity ◽  
Human Health ◽  
Health Effect ◽  
Chemotherapeutic Agents ◽  
Immunomodulatory Activity ◽  
Areca Nut ◽  
Areca Catechu ◽  
Aceh Province ◽  
Potential Activity

Many herbs have been discovered to be potential sources of the antitumor and immunomodulatory drug. Areca nut (Areca catechu L.) has a high content of phenols and flavonoids and is highly related to antioxidant activity. Areca nut is a traditional herbal medicine that is popular around Indonesia, India, Thailand, and Taiwan. However, data on its effect on human health showed various results. This chapter’s aim to review the phytochemical and polyphenolic content, the molecular structure of bioactive compounds, the side effect of the crude extract, the role of catechin in cancer mechanism, the antioxidant activity, the cytotoxicity, and immunomodulatory activity of the areca nut. Areca nuts from Aceh province in Indonesia, contain flavonoids, phenolics, catechin, quercetin, and a small percentage of tannins which contribute to antioxidant activity. The areca nut has anticancer potential activity so it can be used in combination with chemotherapeutic agents to enhance the effect at lower doses and thus minimize chemotherapy-induced toxicity. Areca nuts also show immunomodulatory activity which can increase the body’s immune system.

scholarly journals Areca catechu nut: a double-edged sword

2017 ◽  
Vol 6 (3) ◽  
pp. 700
Author(s):  
Ajitha Sharma
Keyword(s):  
Antioxidant Activity ◽  
Spatial Memory ◽  
East Asian ◽  
Areca Nut ◽  
Betel Nut ◽  
Asian Countries ◽  
Learning Behaviour ◽  
Areca Catechu ◽  
In Vitro Antioxidant Activity

The chewing of Areca catechu nut, more popularly known as betel nut, is one of the ancient traditions in India and other South-east Asian countries. Other than its role played in religious practices and cultural rituals, areca nut has also been described to have various health benefits, particularly antihelminthic and antiviral properties, in the traditional systems of medicine like Ayurveda. It was observed to produce an antidepressant-like effect by elevating the serotonin and norepinephrine levels in hippocampus of rats. It also exhibited analgesic and anti-inflammatory activities in mice, and displayed in vitro antioxidant activity. Furthermore, it was found to improve spatial memory and learning behaviour in rats.

scholarly journals POTENTIAL ANTIOXIDANT AND CYTOTOXIC ACTIVITIES OF ARECA NUT (ARECA CATECHU LINN.) EXTRACT IN HUMAN ORAL SQUAMOUS CELL CARCINOMA AND KERATINOCYTE CELLS

2017 ◽  
Vol 10 (10) ◽  
pp. 286 ◽  
Author(s):  
Liza Meutia Sari ◽  
Gus Permana Subita ◽  
Elza Ibrahim Auerkari
Keyword(s):  
Antioxidant Activity ◽  
Total Phenolic ◽  
Cytotoxic Activities ◽  
Hacat Cells ◽  
Areca Nut ◽  
Flavonoid Content ◽  
Flavonoid Compounds ◽  
Areca Catechu ◽  
Phenolic And Flavonoid ◽  
Phenolic And Flavonoid Compounds

  Objectives: Areca (betel, Areca catechu) nuts have been reported to show high contents of phenolic and flavonoid compounds with associated antioxidant activity. This study was undertaken to assess the anticancer potential of areca nut extract on human squamous carcinoma (HSC)-3, HSC-2, and human keratinocyte (HaCat) cancer cell lines, and to elucidate the mechanism of its action.Methods: In this study, the areca nut extract was prepared in 96% ethanol and analyzed for the total phenolic and flavonoid content, 2,2-diphenyl-1- picrylhydrazyl (DPPH) assay, and 5-(3-carboxymethoxyphenyl)-2-(4,5-dimenthylthiazoly)-3-(4-sulfophenyl)tetrazolium inner salt assay.Results: The indicated total phenolic content of the areca nut extract was 80.3 mg tannic acid equivalent/g and total flavonoid content 238.5 mg catechin equivalent/g. The extract showed good scavenging activity against DPPH radical (efficient concentration 15.95±0.84 μg/ml). The extract showed higher toxicity on HSC-3 than HSC-2 cells, IC50 was 164.06 μg/ml in HSC-3 versus 629.50 μg/ml in HSC-2. The extract had no cytotoxic effect on HaCat cells.Conclusions: The antioxidant activity of the areca nut extract might be associated with the presence of high content of phenolic and flavonoid compounds. The extract showed selective cytotoxicity against HSC-3 and HSC-2 cells but not on HaCat cells. Instead, the extract induced high rate of proliferation in HaCat cells.

Effect of Particle Size on the Oil Yield and Catechin Compound Using Accelerated Solvent Extraction

2013 ◽  
Vol 60 (1) ◽  
Author(s):  
Mohd Azizi Che Yunus ◽  
Manzurudin Hasan ◽  
Norasikin Othman ◽  
Siti Hamidah Mohd-Setapar ◽  
Liza Md.-Salleh ◽  
...  
Keyword(s):  
Particle Size ◽  
Solvent Extraction ◽  
Accelerated Solvent Extraction ◽  
Extraction Time ◽  
Oil Yield ◽  
Particle Sizes ◽  
Experimental Range ◽  
Areca Catechu ◽  
Effect Of Particle Size ◽  
Extraction Cycle

Kajian ini bertujuan untuk mengkaji kesan saiz zarah ke atas pengekstrakan sebatian catechin daripada biji Areca catechu L. dengan menggunakan Pengekstrakan Pelarut Terpecut (PPT). Saiz zarah biji Areca catechu dipelbagaikan dari 75 μm sehingga 500 μm. Pengekstrakan telah dijalankan padaparameter tetap iaitu suhu (140oC), tekanan (1500 psi), masa (10 minit), isipadu semburan (60%) dan satu kitaran pengekstrakan, masing-masing. Hasil minyak peratusan yang lebih tinggi adalah 300 mg minyak / gram sampel (30.00% pati minyak) ditemui pada 125 μm. Walaubagaimanapun, kandungan catechin dalam pati minyak hanya 0.0375 mg catechin / gram sampel. Saiz zarah yang terbaik dalam julat uji kaji ini telah dikenal pasti pada 500 μm yang memberikan kandungan catechin yang tinggi iaitu 0.0515 mg catechin / gram sampel dari 247.5 mg minyak / gram sampel (24.75% pati minyak). Kata kunci: Saiz zarah; catechin; LC-MS-TOF; pengekstrakan pelarut terpecut The purpose of this work is to investigate the effects of particle size on the extraction of catechincompound from Areca catechu L. seeds by using Accelerated Solvent Extraction (ASE). The particle sizes of Areca catechu L. seeds are varied from 75 µm until 500 µm. The extraction is conducted at fixed parameters which are temperature (140oC), pressure (1500 psi), extraction time (10 minutes), flush volume (60%) and the static cycle is done for 1 extraction cycle respectively. Higher percentage oil yield of 300mg oil/gram of sample (30.00% oil yield) is found at 125 µm. However, the amount of catechin in oil yields is only 0.0375 mg of catechin/gram of sample. The best of particle size within the experimental range has been identified at 500 µm which gives a high content of catechin with 0.0515 mg Catechin/gram of sample from 247.5 mg oil/gram of sample (24.75% oil yield). Keywords: Particle size; catechin; LC-MS-TOF; accelerated solvent extraction

scholarly journals Ekstraksi senyawa fenolik antioksidan dari daun dan tangkai gambir

2018 ◽  
Vol 12 (2) ◽  
pp. 250
Author(s):  
Margareth Gani ◽  
Yesisca Cuaca ◽  
Aning Ayucitra ◽  
Nani Indraswati
Keyword(s):  
Antioxidant Activity ◽  
Solvent Extraction ◽  
Ethyl Acetate ◽  
Modern Medicine ◽  
Ethanol Solution ◽  
Total Phenolic ◽  
Acetate Solution ◽  
Extraction Temperature ◽  
Extraction Condition ◽  
Solvent Extraction Method

Extraction of phenolic compounds from leaves and stems of uncaria gambirGambir (Uncaria gambir) contains catechin polyphenols (catechins) which is useful as natural antioxidant to counteract free radicals. Gambir may also be used in modern medicine, also as paint or dye clothing. The objective of this research was to study the effect of type of solvent, solvent concentration, and extraction temperature to the yield and Total Phenolic Content (TPC) of extracts. Gambir was firstly blended without the addition of water prior to extraction using a solvent extraction method. The filtrate was separated from solid residue and evaporated to obtain extract powder. The yield and TPC of the resulting extract powder were then analyzed. Antioxidant activity and catechins content of extracts were also studied. As result, the following extraction condition gave extracts with the highest yield and TPC: with 50% ethanol solution as solvent at 75 oC gave 11.12% and 52.352 g GAE/100 g gambir extract, respectively; whilst with 70% ethyl acetate solution as solvent at 65 oC gave 5.28% and 59.346 g GAE/100 g gambir extract, respectively. The antioxidant activity of extracts, calculated as IC50, for solvent ethanol and ethyl acetate were as follows: 8.9 mg extract/mL and 13.8 mg extract/mL, respectively; whilst the catechins content of extracts were 62,18% and 44,85%, respectively.Keywords: Uncaria gambir, solvent extraction, catechins, antioxidants AbstrakGambir (Uncaria gambir) mengandung polifenol katekin (catechin) yang bermanfaat sebagai bahan antioksidan alami yang dapat menangkal radikal bebas. Selain itu, gambir juga dimanfaatkan dalam pembuatan obat-obatan modern dan pewarna cat atau pakaian. Penelitian ini bertujuan untuk mempelajari pengaruh jenis pelarut, konsentrasi pelarut, dan suhu ekstraksi terhadap perolehan dan kandungan senyawa fenolik (TPC) ekstrak gambir. Daun dan tangkai gambir mula-mula diblender tanpa penambahan air. Proses ekstraksi gambir dilakukan pada berbagai jenis pelarut, konsentrasi pelarut, dan suhu ekstraksi denganmetode ekstraksi pelarut. Setelah padatan dipisahkan dengan pelarutnya, pelarut diuapkan untuk mendapatkan serbuk ekstrak gambir. Serbuk ekstrak gambir dianalisis perolehan dan kandungan senyawa fenoliknya. Aktivitas antioksidan dan kadar katekin ekstrak juga dianalisis. Ekstrak daun gambir dengan perolehan dan TPC tertinggi untuk pelarut etanol (11,12% dan 52,352 g GAE/100 g gambir ekstrak) diperoleh pada konsentrasi etanol 50% dan suhu ekstraksi 75oC. Untuk pelarut etil asetat, perolehan dan TPC tertinggi (5,28% dan 59,346 g GAE/100 g gambir ekstrak) diperoleh pada konsentrasi etil asetat 70% dan suhu ekstraksi 65 oC. Pada masing-masing kondisi terbaik tersebut, ekstrak yang didapat memiliki aktivitas antioksidan (dalam IC50) dan kadar katekin sebesar 8,9 mg ekstrak/mL dan 62,18% untuk ekstraksi dengan pelarut etanol serta 13,8 mg ekstrak/mL dan 44,85% untuk pelarut etil asetat.Kata kunci: Uncaria gambir, ekstraksi pelarut, katekin, antioksidan 

scholarly journals The anthocyanin pigment extract from red rose as antibacterial agent

2018 ◽  
Vol 14 (1-2) ◽  
pp. 184-187
Author(s):  
Elfi Anis Saati ◽  
Annisa Dyah Pusparini ◽  
Mochammad Wachid ◽  
Sri Winarsih
Keyword(s):  
Escherichia Coli ◽  
Antioxidant Activity ◽  
Solvent Extraction ◽  
Shelf Life ◽  
Antibacterial Agent ◽  
Block Design ◽  
Pseudomonas Sp ◽  
Anthocyanin Pigment ◽  
Extraction Solvent ◽  
Pigment Extract

Red rose consisting of anthocyanin pigment has been used as an antibacterial agent. However, there is no study on the anthocyanin pigment extract from red rose as the antibacterial agent. The effectiveness of the antibacterial agent can be affected by the solvent extraction and the flower shelf life. Here, we report the effects of solvent extraction and red rose (Rosa sp.) shelf life on the antibacterial activity. Red rose concentrated extraction and randomized complete block design factorial was carefully used with factors of long display and solvent extraction. The extraction solvent (P) comprised of water, ethanol, and mixture of water-ethanol (1 : 1), while the red rose shelf life (M) consisted of 0, 2, 4 and 6 days. Moreover, pH, antioxidant activity and minimum bactericidal concentration (MBC) with four variations of concentrated concentration (100%, 50%, 25% and 12.5%) on Escherichia coli, Salmonella thypi, and Pseudomonas sp. were analyzed. After two days of shelf life using water as the solvent for extraction, the antioxidant activity achieved 79% at pH of 2.5. It was also demonstrated that it was able to kill all the investigated bacterias, which were Escherichia coli, Salmonella thypi, and Pseudomonas sp. with concentrated concentrations of 100%, 50% and 25%. When the concentrated concentration was 12.5%, the MBC value was constantly found to be 1.39 × 108 cfu/g for Escherichia coli and 9.53 × 107 cfu/g for Salmonella thypi.

scholarly journals The Effect of Administration of Extract from Areca Nut Seeds (Areca Catechu L) on the Estradiol and Estrus Cycle Balb/C Female Rats

2020 ◽  
Vol 1477 ◽  
pp. 062026
Author(s):  
T Rina Aritonang ◽  
R Natzir ◽  
A Wardihan Sinrang ◽  
M Nasrum Massi ◽  
M Hatta ◽  
...  
Keyword(s):  
Female Rats ◽  
Areca Nut ◽  
Estrus Cycle ◽  
Areca Catechu

scholarly journals First Report of Leaf Sheath Rot of Areca Nut (Areca catechu) Caused by Athelia rolfsii from Tripura, India

Plant Disease ◽  
2020 ◽  
Vol 104 (11) ◽  
pp. 3070
Author(s):  
Deeba Kamil ◽  
Amar Bahadur ◽  
Prasenjit Debnath ◽  
Anjali Kumari ◽  
Shiv Pratap Choudhary ◽  
...  
Keyword(s):  
Leaf Sheath ◽  
Areca Nut ◽  
First Report ◽  
Athelia Rolfsii ◽  
Areca Catechu ◽  
Sheath Rot
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