A sensitive and selective direct competitive enzyme-linked immunosorbent assay for fast detection of Sudan I in food samples

2011 ◽  
Vol 91 (10) ◽  
pp. 1836-1842 ◽  
Author(s):  
Yuzhen Wang ◽  
Hong Yang ◽  
Bin Wang ◽  
Anping Deng
Keyword(s):  
Immunosorbent Assay ◽  
Food Samples ◽  
Enzyme Linked Immunosorbent Assay ◽  
Fast Detection ◽  
Sudan I

Development of a Highly Sensitive and Specific Enzyme-Linked Immunosorbent Assay for Detection of Sudan I in Food Samples

2007 ◽  
Vol 55 (16) ◽  
pp. 6424-6430 ◽  
Author(s):  
Dan Han ◽  
Meng Yu ◽  
Dietmar Knopp ◽  
Reinhard Niessner ◽  
Mei Wu ◽  
...  
Keyword(s):  
Immunosorbent Assay ◽  
Food Samples ◽  
Enzyme Linked Immunosorbent Assay ◽  
Specific Enzyme ◽  
Highly Sensitive ◽  
Sudan I

Development of a Chemiluminescence ELISA for the Detection of Sudan I in Chilli Powder

2013 ◽  
Vol 830 ◽  
pp. 310-313
Author(s):  
Yan Fan ◽  
Li Xin Zhu ◽  
Ren Rong Liu ◽  
Long Xu ◽  
Wei Meng
Keyword(s):  
Immunosorbent Assay ◽  
Recovery Rate ◽  
Limit Of Detection ◽  
Food Products ◽  
Food Samples ◽  
Linear Range ◽  
Enzyme Linked Immunosorbent Assay ◽  
Sudan I ◽  
Antigen Antibody

A chemiluminescence enzyme-linked immunosorbent assay (CL-ELISA) was developed for the detection of Sudan I in food products. The CL-ELISA conditions, such as the concentration of antigen, antibody and goat anti-mouse IgG-HRP, were optimized. The optimized CL-ELISA allowed the Sudan I detection in a linear range of 0.625-10 ng mL-1, the IC50 was 3.3ng mL-1 and the limit of detection (LOD) was 0.31 ng mL-1. The method showed good recoveries with spiked chilli powder. The recovery rate in a batch range from 73-109.6%, and the recovery rate between the batch range from 78-109.24%. The proposed method proved to be efficient for the detection of Sudan I in food samples.

Development of a new monoclonal antibody based direct competitive enzyme-linked immunosorbent assay for detection of brevetoxins in food samples

2010 ◽  
Vol 118 (2) ◽  
pp. 467-471 ◽  
Author(s):  
Yu Zhou ◽  
Yan-Song Li ◽  
Feng-Guang Pan ◽  
Yuan-Yuan Zhang ◽  
Shi-Ying Lu ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Immunosorbent Assay ◽  
Food Samples ◽  
Enzyme Linked Immunosorbent Assay

Hapten Synthesis and Development of a Competitive Indirect Enzyme-Linked Immunosorbent Assay for Acrylamide in Food Samples

2014 ◽  
Vol 62 (29) ◽  
pp. 7078-7084 ◽  
Author(s):  
Jing Wu ◽  
Yu-Dong Shen ◽  
Hong-Tao Lei ◽  
Yuan-Ming Sun ◽  
Jin-Yi Yang ◽  
...  
Keyword(s):  
Immunosorbent Assay ◽  
Food Samples ◽  
Enzyme Linked Immunosorbent Assay ◽  
Hapten Synthesis

Validated Sandwich Enzyme-Linked Immunosorbent Assay for Casein and Its Application to Retail and Milk-Allergic Complaint Foods

2004 ◽  
Vol 67 (9) ◽  
pp. 1933-1938 ◽  
Author(s):  
SUSAN L. HEFLE ◽  
DEBRA M. LAMBRECHT
Keyword(s):  
Immunosorbent Assay ◽  
Milk Proteins ◽  
Food Samples ◽  
Enzyme Linked Immunosorbent Assay ◽  
Food Ingredients ◽  
Milk Contamination ◽  
Quality Control Tool ◽  
Sandwich Type ◽  
Life Threatening ◽  
Phosphate Buffered Saline

Cows' milk is a commonly allergenic food. Cross-contamination of milk proteins into nondairy, kosher-pareve foods prepared on shared processing equipment can cause severe, life-threatening reactions in milk-allergic individuals. A sandwich-type enzyme-linked immunosorbent assay (ELISA; 96-well plate format) was developed for the detection of undeclared casein in foods. Rabbit anti-casein antibodies were used as the capture reagent. Food samples and standards were ground, extracted in 0.01 M phosphate-buffered saline, clarified by centrifugation, and added to the wells. Goat anti-casein antibodies were employed as the detector antibody, and the amount of antibody bound was determined with a commercial rabbit anti-goat immunoglobulin conjugated to alkaline phosphatase, with subsequent substrate reaction. Antibodies developed were specific to casein, with no cross-reaction observed with 30 foods and food ingredients. Non–milk-containing products such as fruit juices, fruit juice bars, sorbets, and dark and pareve-labeled chocolate were purchased from June 2002 through June 2003. In addition, samples allegedly causing eight milk-allergic consumer complaints were analyzed. The ELISA had a detection limit of less than 0.5 ppm of casein. The casein content in the analyzed foods ranged from less than 0.5 ppm to more than 40,000 ppm casein; undeclared casein residues were found in all of the samples implicated in allergic reactions. The levels of milk contamination in some of the other surveyed products could also be hazardous for milk-allergic consumers. This ELISA method provides a useful quality control tool for the food industry and could also be used as a validation of kosher-pareve status.

scholarly journals Investigation of the effect of hapten heterology in the enzyme-linked immunosorbent assay for Sudan I

2013 ◽  
Vol 26 (1) ◽  
pp. 13-25 ◽  
Author(s):  
Jiafa Xu ◽  
Zuozhou Fan ◽  
Xiaobo Huang ◽  
Yixiang Cheng ◽  
Yan Lu ◽  
...  
Keyword(s):  
Immunosorbent Assay ◽  
Enzyme Linked Immunosorbent Assay ◽  
Sudan I
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