Hapten Synthesis and Development of a Competitive Indirect Enzyme-Linked Immunosorbent Assay for Acrylamide in Food Samples

2014 ◽  
Vol 62 (29) ◽  
pp. 7078-7084 ◽  
Author(s):  
Jing Wu ◽  
Yu-Dong Shen ◽  
Hong-Tao Lei ◽  
Yuan-Ming Sun ◽  
Jin-Yi Yang ◽  
...  
Keyword(s):  
Immunosorbent Assay ◽  
Food Samples ◽  
Enzyme Linked Immunosorbent Assay ◽  
Hapten Synthesis

Development of a new monoclonal antibody based direct competitive enzyme-linked immunosorbent assay for detection of brevetoxins in food samples

2010 ◽  
Vol 118 (2) ◽  
pp. 467-471 ◽  
Author(s):  
Yu Zhou ◽  
Yan-Song Li ◽  
Feng-Guang Pan ◽  
Yuan-Yuan Zhang ◽  
Shi-Ying Lu ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Immunosorbent Assay ◽  
Food Samples ◽  
Enzyme Linked Immunosorbent Assay

Validated Sandwich Enzyme-Linked Immunosorbent Assay for Casein and Its Application to Retail and Milk-Allergic Complaint Foods

2004 ◽  
Vol 67 (9) ◽  
pp. 1933-1938 ◽  
Author(s):  
SUSAN L. HEFLE ◽  
DEBRA M. LAMBRECHT
Keyword(s):  
Immunosorbent Assay ◽  
Milk Proteins ◽  
Food Samples ◽  
Enzyme Linked Immunosorbent Assay ◽  
Food Ingredients ◽  
Milk Contamination ◽  
Quality Control Tool ◽  
Sandwich Type ◽  
Life Threatening ◽  
Phosphate Buffered Saline

Cows' milk is a commonly allergenic food. Cross-contamination of milk proteins into nondairy, kosher-pareve foods prepared on shared processing equipment can cause severe, life-threatening reactions in milk-allergic individuals. A sandwich-type enzyme-linked immunosorbent assay (ELISA; 96-well plate format) was developed for the detection of undeclared casein in foods. Rabbit anti-casein antibodies were used as the capture reagent. Food samples and standards were ground, extracted in 0.01 M phosphate-buffered saline, clarified by centrifugation, and added to the wells. Goat anti-casein antibodies were employed as the detector antibody, and the amount of antibody bound was determined with a commercial rabbit anti-goat immunoglobulin conjugated to alkaline phosphatase, with subsequent substrate reaction. Antibodies developed were specific to casein, with no cross-reaction observed with 30 foods and food ingredients. Non–milk-containing products such as fruit juices, fruit juice bars, sorbets, and dark and pareve-labeled chocolate were purchased from June 2002 through June 2003. In addition, samples allegedly causing eight milk-allergic consumer complaints were analyzed. The ELISA had a detection limit of less than 0.5 ppm of casein. The casein content in the analyzed foods ranged from less than 0.5 ppm to more than 40,000 ppm casein; undeclared casein residues were found in all of the samples implicated in allergic reactions. The levels of milk contamination in some of the other surveyed products could also be hazardous for milk-allergic consumers. This ELISA method provides a useful quality control tool for the food industry and could also be used as a validation of kosher-pareve status.

Development of a direct competitive enzyme-linked immunosorbent assay for parathion residue in food samples

2009 ◽  
Vol 393 (1) ◽  
pp. 88-94 ◽  
Author(s):  
Wen-Jun Gui ◽  
Yi-Hua Liu ◽  
Chun-Mei Wang ◽  
Xiao Liang ◽  
Guo-Nian Zhu
Keyword(s):  
Immunosorbent Assay ◽  
Food Samples ◽  
Enzyme Linked Immunosorbent Assay

scholarly journals Fungal Diversity and Aflatoxins in Cereals and Cassava-Based Foods From Ondo State, Nigeria

2021 ◽  
Author(s):  
Daniella O. Ekpakpale ◽  
Bart Kraak ◽  
Martin Meijer ◽  
Kolawole I. Ayeni ◽  
Jos Houbraken ◽  
...  
Keyword(s):  
Aspergillus Flavus ◽  
Molecular Analysis ◽  
Immunosorbent Assay ◽  
Fungal Diversity ◽  
Food Samples ◽  
Enzyme Linked Immunosorbent Assay ◽  
Mitigation Options ◽  
Predominant Species ◽  
Consumer Safety ◽  
Ondo State

Cereals and cassava-based foods serve as major dietary sources for several households in Nigeria. However, these foods are highly prone to contamination by moulds and aflatoxins owing to poor storage and vending practices. We therefore studied the fungal diversity of maize, cassava-based flour (pupuru) and rice vended in markets from Ondo state, Nigeria, and assessed the aflatoxin levels of these using Enzyme-Linked Immunosorbent Assay. Fungi were detected in 93 (88 %) of the 106 food samples. Molecular analysis of 65 representative isolates revealed 26 species belonging to 5 genera: Aspergillus (80.9 %), Penicillium (15.4 %) and Talaromyces (1.9 %) in the Ascomycota; Syncephalastrum (1.2 %) and Lichtheimia (0.6%) in Mucoromycota. Aspergillus flavus was the predominant species in the food samples. Aflatoxins were found in 98 % of the 42 representative food samples and about one half (49.8 %) exceeded the 10 μg/kg threshold adopted in Nigeria for total aflatoxins. Integrated mitigation options, including at post-harvest stages, are suggested to ensure consumer safety.

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