Development of a Highly Sensitive and Specific Enzyme-Linked Immunosorbent Assay for Detection of Sudan I in Food Samples

2007 ◽  
Vol 55 (16) ◽  
pp. 6424-6430 ◽  
Author(s):  
Dan Han ◽  
Meng Yu ◽  
Dietmar Knopp ◽  
Reinhard Niessner ◽  
Mei Wu ◽  
...  
Keyword(s):  
Immunosorbent Assay ◽  
Food Samples ◽  
Enzyme Linked Immunosorbent Assay ◽  
Specific Enzyme ◽  
Highly Sensitive ◽  
Sudan I

Development of a highly sensitive and robust Cor a 9 specific enzyme-linked immunosorbent assay for the detection of hazelnut traces

2011 ◽  
Vol 708 (1-2) ◽  
pp. 116-122 ◽  
Author(s):  
Stanislav A. Trashin ◽  
Tatiana Cucu ◽  
Bart Devreese ◽  
Annemie Adriaens ◽  
Bruno De Meulenaer
Keyword(s):  
Immunosorbent Assay ◽  
Enzyme Linked Immunosorbent Assay ◽  
Specific Enzyme ◽  
Highly Sensitive

Development of a Chemiluminescence ELISA for the Detection of Sudan I in Chilli Powder

2013 ◽  
Vol 830 ◽  
pp. 310-313
Author(s):  
Yan Fan ◽  
Li Xin Zhu ◽  
Ren Rong Liu ◽  
Long Xu ◽  
Wei Meng
Keyword(s):  
Immunosorbent Assay ◽  
Recovery Rate ◽  
Limit Of Detection ◽  
Food Products ◽  
Food Samples ◽  
Linear Range ◽  
Enzyme Linked Immunosorbent Assay ◽  
Sudan I ◽  
Antigen Antibody

A chemiluminescence enzyme-linked immunosorbent assay (CL-ELISA) was developed for the detection of Sudan I in food products. The CL-ELISA conditions, such as the concentration of antigen, antibody and goat anti-mouse IgG-HRP, were optimized. The optimized CL-ELISA allowed the Sudan I detection in a linear range of 0.625-10 ng mL-1, the IC50 was 3.3ng mL-1 and the limit of detection (LOD) was 0.31 ng mL-1. The method showed good recoveries with spiked chilli powder. The recovery rate in a batch range from 73-109.6%, and the recovery rate between the batch range from 78-109.24%. The proposed method proved to be efficient for the detection of Sudan I in food samples.

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