Lipids of potato tubers. II. Lipid-degrading enzymes in different varieties of potato tuber

1973 ◽  
Vol 24 (5) ◽  
pp. 623-627 ◽  
Author(s):  
T. Galliard ◽  
J. A. Matthew
Keyword(s):  
Potato Tuber ◽  
Potato Tubers ◽  
Degrading Enzymes

scholarly journals Expression Levels of the γ-Glutamyl Hydrolase I Gene Predict Vitamin B9 Content in Potato Tubers

Agronomy ◽  
2019 ◽  
Vol 9 (11) ◽  
pp. 734 ◽  
Author(s):  
Bruce R. Robinson ◽  
Carolina Garcia Salinas ◽  
Perla Ramos Parra ◽  
John Bamberg ◽  
Rocio I. Diaz de la Garza ◽  
...  
Keyword(s):  
Potato Tuber ◽  
Tomato Fruit ◽  
Genetically Engineered ◽  
Food Crops ◽  
Great Success ◽  
Rice Grain ◽  
Rna Seq ◽  
Potato Tubers ◽  
Vitamin B9 ◽  
Folate Biosynthesis

Biofortification of folates in staple crops is an important strategy to help eradicate human folate deficiencies. Folate biofortification using genetic engineering has shown great success in rice grain, tomato fruit, lettuce, and potato tuber. However, consumers’ skepticism, juridical hurdles, and lack of economic model have prevented the widespread adoption of nutritionally-enhanced genetically-engineered (GE) food crops. Meanwhile, little effort has been made to biofortify food crops with folate by breeding. Previously we reported >10-fold variation in folate content in potato genotypes. To facilitate breeding for enhanced folate content, we attempted to identify genes that control folate content in potato tuber. For this, we analyzed the expression of folate biosynthesis and salvage genes in low- and high-folate potato genotypes. First, RNA-Seq analysis showed that, amongst all folate biosynthesis and salvage genes analyzed, only one gene, which encodes γ-glutamyl hydrolase 1 (GGH1), was consistently expressed at higher levels in high- compared to low-folate segregants of a Solanum boliviense Dunal accession. Second, quantitative PCR showed that GGH1 transcript levels were higher in high- compared to low-folate segregants for seven out of eight pairs of folate segregants analyzed. These results suggest that GGH1 gene expression is an indicator of folate content in potato tubers.

THE FLUORESCENCE OF FROZEN POTATO TUBER AND APPLE FRUIT TISSUE UNDER ULTRA-VIOLET LIGHT

1945 ◽  
Vol 23c (2) ◽  
pp. 76-78 ◽  
Author(s):  
W. Newton ◽  
W. Jones
Keyword(s):  
Potato Tuber ◽  
Ultra Violet ◽  
Apple Fruit ◽  
Potato Tubers ◽  
Tuber Tissue ◽  
Fruit Tissue ◽  
Potato Tuber Tissue ◽  
Violet Light ◽  
Apple Tissue ◽  
Acetone Extracts

Freshly cut sections of frozen potato tuber and apple fruit tissue exhibit a brilliant fluorescence when examined in a dark room under an ultra-violet (Stroblite) lamp. The fluorescence disappears from the apple tissue upon thawing but is retained in potato tuber tissue. Thus the ultra-violet lamp serves as a useful diagnostic means of detecting frost or low temperature injury in potato tubers. Many other plant tissues were frozen and examined, but proved to be non-fluorescent. Acetone extracts of both normal and frozen potato tuber tissue were fluorescent, but similar extracts of both frozen and normal apple fruit were not. Although normal potato tissue is non-fluorescent and frozen tissue is brightly fluorescent, no differences were found in the brightness of the acetone extracts.

scholarly journals Variety-specificity of soluble proteins of potato tubers

2015 ◽  
Vol 43 (4) ◽  
pp. 491-498 ◽  
Author(s):  
Marta Czupryn ◽  
Kazimierz Toczko
Keyword(s):  
Potato Tuber ◽  
Genetic Research ◽  
Electrophoretic Pattern ◽  
Electrophoretic Analysis ◽  
Disc Electrophoresis ◽  
The Other ◽  
Potato Tubers ◽  
Genetic Origin ◽  
Electrophoretic Patterns ◽  
Taxonomic Studies

Separation of soluble tuber proteins from six potato clones and twelve varieties cultivated in Poland has been accomplished by disc electrophoresis. It was found that electrophoretic pattern was unique for a given clone or variety. Data obtained confirm results of the other authors for the other varieties and indicate that electrophoretic analysis of potato tuber proteins can be a useful method for taxonomic studies. Such analysis however cannot be used for genetic research since no correlations were found between electrophoretic patterns and genetic origin of respective clones and varieties.

scholarly journals Evaluation of Some Potential Botanicals to Control Potato Tuber Moth, (Phthorimaea operculella) under Storage Condition at Bako, Western Ethiopia

2012 ◽  
Vol 1 (1) ◽  
pp. 14-18 ◽  
Author(s):  
Aschalew Sisay ◽  
Ahmed Ibrahim
Keyword(s):  
Potato Tuber ◽  
Eucalyptus Globulus ◽  
Agricultural Research ◽  
Block Design ◽  
Storage Condition ◽  
Lantana Camara ◽  
The Other ◽  
Potato Tuber Moth ◽  
Potato Tubers ◽  
Cropping Seasons

Lantana camara, Eucalyptus globulus, Tagetus minuta, Pyrethrum flowers and Azaddiractha indica, were evaluated against potato tuber moth damage with two checks (Diazinon 60% EC and untreated check) at Bako Agricultural Research Center in 2007/2008 and 2008/2009 cropping seasons. A total of 21 huts (cottages) were constructed from locally available materials.   Inside each cottage (hut), 1m 2 bed was constructed 65 cm above the ground. Six hundred fifty (650) potato tubers were stored on the bed of each hut in two layers one over the other. The treatments were arranged in a Randomized Complete Block Design (RCBD) with three replications. Plants powder and Diazinon 60% EC were applied respectively, at the rate of 50 gm / bed and 3.5ml / bed at two months’ interval. The treatments that showed promising effects were further advanced on a large bed (2m x3m). Three thousand nine hundred (3900) potato tubers were stored on large beds and 150gm powders were used for each bed. Evaluations were made at the interval of 15 days for 7 consecutive months. Analysis of variance showed that number of potatoes infested and damaged by potato tuber moth was significantly (P0.05) lower in the Lantana camara, Eucalyptus globulus and Pyrethrum flowers than the other treatments, which are similar to standard check. From the study it can be concluded that Lantana camara, E. globulus andPyrethrum flowers can be used to protect seed potatoes from potato tuber moth damage in storage.

scholarly journals Possibility of Limiting Mineral Fertilization in Potato Cultivation by Using Bio-fertilizer and Its Influence on Protein Content in Potato Tubers

Agriculture ◽  
2020 ◽  
Vol 10 (10) ◽  
pp. 442
Author(s):  
Dorota Wichrowska ◽  
Małgorzata Szczepanek
Keyword(s):  
Amino Acids ◽  
Potato Tuber ◽  
Farmyard Manure ◽  
Fertilization Rate ◽  
Organic Fertilizers ◽  
Mineral Fertilizers ◽  
Catch Crop ◽  
Potato Tubers ◽  
Mineral Fertilization ◽  
Tuber Protein

Potato protein is a valuable source of essential plant-derived amino acids, the composition of which is similar to that of chicken egg protein considering the amino acid reference. Many factors used in potato cultivation can modify its composition. The use of bio-fertilizers in potato growing offers a possibility of a better use of minerals from soil and organic sources and reducing the need for mineral fertilizers by activating minerals present in soil. The effect can be to improve not only the potato tuber yield but also the nutritional value. The aim of this study has been to determine the hanges in the content of crude protein and the composition of amino acids in potato tubers, depending on the application of the bio-fertilizer (UGmax), organic fertilizers (pea as a catch crop, straw, and farmyard manure (FYM)) as well as mineral fertilization (100% and 50% of the reference rate). The application of bio-fertilizer significantly increased the content of essential and non-essential amino acids in potato tuber protein. With the half-decreased mineral fertilization rate, bio-fertilizer most effectively increased the content of tyrosine, methionine, asparagine in potato tuber protein in the treatments with FYM or with a catch crop as well as without organic fertilization.

Potato tubers exhibit both homolytic and heterolytic hydroperoxide fatty acid-cleaving activities

2000 ◽  
Vol 28 (6) ◽  
pp. 853-855 ◽  
Author(s):  
M.-L Fauconnier ◽  
J. Delcarte ◽  
P. Hoyaux ◽  
P. du Jardin ◽  
M. Marlier
Keyword(s):  
Fatty Acid ◽  
Potato Tuber ◽  
Linolenic Acid ◽  
Hplc Analysis ◽  
Potato Tubers ◽  
Almost All ◽  
Fatty Acid Hydroperoxides ◽  
Tuber Extract

The action of a crude potato-tuber extract on 9- and 13-hydroperoxides of linoleic and linolenic acids was investigated. HPLC analysis revealed that 50% of the 9-hydroperoxide isomers and almost all the 13-hydroperoxide isomers were rapidly enzymically metabolized. No degradation of fatty acid hydroperoxides was observed with a thermally denatured enzymic extract. GC-MS identification of the volatiles formed by the reaction revealed that no volatiles were detected from the 9-hydroperoxide isomers, whereas 13-hydroperoxide of linolenic acid was cleaved into (Z)-3-hexenal, pentenols or dimers of pentene.

Electrical impedance analysis in plant tissues: The effect of freeze-thaw injury on the electrical properties of potato tuber and carrot root tissues

1992 ◽  
Vol 72 (2) ◽  
pp. 545-553 ◽  
Author(s):  
M. I. N. Zhang ◽  
J. H. M. Willison
Keyword(s):  
Plasma Membrane ◽  
Potato Tuber ◽  
Electrical Impedance ◽  
Membrane Capacitance ◽  
Potato Tubers ◽  
Carrot Root ◽  
Freeze Thaw ◽  
Intracellular Resistance ◽  
Root Tissues ◽  
Extracellular Resistance

Electrical impedances in the range 100 Hz to 800 KHz were measured in potato tubers and carrot root cortex both before and after freeze-thaw cycles. These impedance data were analyzed using equivalent circuit modelling based on complex nonlinear least squares (CNLS) (Zhang and Willison 1991a). After freezing to and thawing from −3, −6, −9 and −12 °C, carrot root tissues were best characterized by a double shell model composed of extracellular resistance, cytoplasmic resistance, plasma membrance capacitance, vacuole interior resistance, and tonoplast capacitance. Although freeze–thaw cycles to −3 or −6 °C did not kill the carrot tissues, extracellular resistance and vacuole interior resistance were halved relative to control tissues, and cytoplasmic resistance decreased to a third of the control value. Plasma membrane capacitance and tonoplast capacitance were not affected by the −3 and −6 °C noninjurious stresses. After carrot root or potato tuber tissues had been killed by freezing (−3 °C for potato tuber and −18 °C for carrot), the tissues were best represented by a single-shell model composed of extravesicular resistance, intravesicular resistance, and vesicle membrane. Compared with unfrozen controls, freeze-killed potato tubers were characterized by a 30-fold decrease in extracellular resistance, a 7-fold decrease in intracellular resistance, and a 10-fold decrease in plasma membrane capacitance. Freeze-killed carrot roots were characterized by a 30-fold decrease in extracellular resistance, 3-fold decrease in intracellular resistance, and 3.5-fold decrease in plasma membrane capacitance. These results are compatible with massive rupturing of protoplasts during lethal freeze–thaw injury.Key words: Solanum tuberosum L., Daucus carota L., freeze–thaw injury, electrical impedance

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